Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo.
Stimulation of specific neurons expressing opsins in a targeted region to manipulate brain function has proved to be a powerful tool in neuroscience. However, the use of visible light for optogenetic stimulation is invasive due to low penetration depth and tissue damage owing to larger absorption an...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4226470?pdf=render |
| _version_ | 1818034925110034432 |
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| author | Kamal R Dhakal Ling Gu Shivaranjani Shivalingaiah Torry S Dennis Samara A Morris-Bobzean Ting Li Linda I Perrotti Samarendra K Mohanty |
| author_facet | Kamal R Dhakal Ling Gu Shivaranjani Shivalingaiah Torry S Dennis Samara A Morris-Bobzean Ting Li Linda I Perrotti Samarendra K Mohanty |
| author_sort | Kamal R Dhakal |
| collection | DOAJ |
| description | Stimulation of specific neurons expressing opsins in a targeted region to manipulate brain function has proved to be a powerful tool in neuroscience. However, the use of visible light for optogenetic stimulation is invasive due to low penetration depth and tissue damage owing to larger absorption and scattering. Here, we report, for the first time, in-depth non-scanning fiber-optic two-photon optogenetic stimulation (FO-TPOS) of neurons in-vivo in transgenic mouse models. In order to optimize the deep-brain stimulation strategy, we characterized two-photon activation efficacy at different near-infrared laser parameters. The significantly-enhanced in-depth stimulation efficiency of FO-TPOS as compared to conventional single-photon beam was demonstrated both by experiments and Monte Carlo simulation. The non-scanning FO-TPOS technology will lead to better understanding of the in-vivo neural circuitry because this technology permits more precise and less invasive anatomical delivery of stimulation. |
| first_indexed | 2024-12-10T06:46:54Z |
| format | Article |
| id | doaj.art-c32ed4f4b98a49748e4cdc18f52f5923 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T06:46:54Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c32ed4f4b98a49748e4cdc18f52f59232022-12-22T01:58:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11148810.1371/journal.pone.0111488Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo.Kamal R DhakalLing GuShivaranjani ShivalingaiahTorry S DennisSamara A Morris-BobzeanTing LiLinda I PerrottiSamarendra K MohantyStimulation of specific neurons expressing opsins in a targeted region to manipulate brain function has proved to be a powerful tool in neuroscience. However, the use of visible light for optogenetic stimulation is invasive due to low penetration depth and tissue damage owing to larger absorption and scattering. Here, we report, for the first time, in-depth non-scanning fiber-optic two-photon optogenetic stimulation (FO-TPOS) of neurons in-vivo in transgenic mouse models. In order to optimize the deep-brain stimulation strategy, we characterized two-photon activation efficacy at different near-infrared laser parameters. The significantly-enhanced in-depth stimulation efficiency of FO-TPOS as compared to conventional single-photon beam was demonstrated both by experiments and Monte Carlo simulation. The non-scanning FO-TPOS technology will lead to better understanding of the in-vivo neural circuitry because this technology permits more precise and less invasive anatomical delivery of stimulation.http://europepmc.org/articles/PMC4226470?pdf=render |
| spellingShingle | Kamal R Dhakal Ling Gu Shivaranjani Shivalingaiah Torry S Dennis Samara A Morris-Bobzean Ting Li Linda I Perrotti Samarendra K Mohanty Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo. PLoS ONE |
| title | Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo. |
| title_full | Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo. |
| title_fullStr | Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo. |
| title_full_unstemmed | Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo. |
| title_short | Non-scanning fiber-optic near-infrared beam led to two-photon optogenetic stimulation in-vivo. |
| title_sort | non scanning fiber optic near infrared beam led to two photon optogenetic stimulation in vivo |
| url | http://europepmc.org/articles/PMC4226470?pdf=render |
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