Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast
Comparing co-occurring species may provide insights into how aspects of ecology may play a role in influencing their microbial communities. During the 2019 commercial shrimp trawl season off coastal Georgia, swabs of skin, gills, cloaca, and gut were taken for three species of batoids (Butterfly Ray...
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MDPI AG
2024-01-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/9/1/34 |
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| author | Kady Lyons Christine N. Bedore Aaron B. Carlisle Lauren Moniz Timothy L. Odom Rokeya Ahmed Stephen E. Greiman Ryan M. Freedman |
| author_facet | Kady Lyons Christine N. Bedore Aaron B. Carlisle Lauren Moniz Timothy L. Odom Rokeya Ahmed Stephen E. Greiman Ryan M. Freedman |
| author_sort | Kady Lyons |
| collection | DOAJ |
| description | Comparing co-occurring species may provide insights into how aspects of ecology may play a role in influencing their microbial communities. During the 2019 commercial shrimp trawl season off coastal Georgia, swabs of skin, gills, cloaca, and gut were taken for three species of batoids (Butterfly Ray, Bluntnose Stingray, and Atlantic Stingray) and one shark species (Atlantic Sharpnose) for high-throughput sequencing of the V4 region of the bacterial 16S rRNA gene. White muscle was analyzed for stable isotopes (δ<sup>13</sup>C and δ<sup>15</sup>N) to evaluate potential niche overlap in these four sympatric mesopredators. Significant differences were found in both δ<sup>13</sup>C and δ<sup>15</sup>N signatures across species, suggesting a degree of resource partitioning. When examined within tissue type, the host species had a weak effect on β-diversity for cloaca and skin, with no differences found for gill and gut samples. However, network analysis metrics demonstrated a stronger species-specific effect and distinct microbial community relationships were apparent between the shark and batoids, with the former having tighter networks for both internally- and externally-influenced tissues (gut/cloaca and skin/gills, respectively). Despite overlapping habitat use, species’ microbiomes differed in their organizational structuring that paralleled differences in stable isotope results, suggesting a mediating role of species-specific ecology on bacterial microbiomes. |
| first_indexed | 2024-03-08T10:56:36Z |
| format | Article |
| id | doaj.art-c32f007023f8434789775f0afbe2c0eb |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-08T10:56:36Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-c32f007023f8434789775f0afbe2c0eb2024-01-26T16:24:31ZengMDPI AGFishes2410-38882024-01-01913410.3390/fishes9010034Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia CoastKady Lyons0Christine N. Bedore1Aaron B. Carlisle2Lauren Moniz3Timothy L. Odom4Rokeya Ahmed5Stephen E. Greiman6Ryan M. Freedman7Georgia Aquarium, Center for Species Survival, Atlanta, GA 30313, USADepartment of Biology, Georgia Southern University, Statesboro, GA 30460, USASchool of Marine Science and Policy, University of Delaware, Lewes, DE 19958, USADepartment of Biology, Georgia Southern University, Statesboro, GA 30460, USADepartment of Biology, Georgia Southern University, Statesboro, GA 30460, USADepartment of Biology, Georgia Southern University, Statesboro, GA 30460, USADepartment of Biology, Georgia Southern University, Statesboro, GA 30460, USAChannel Islands National Marine Sanctuary, Santa Barbara, CA 93106, USAComparing co-occurring species may provide insights into how aspects of ecology may play a role in influencing their microbial communities. During the 2019 commercial shrimp trawl season off coastal Georgia, swabs of skin, gills, cloaca, and gut were taken for three species of batoids (Butterfly Ray, Bluntnose Stingray, and Atlantic Stingray) and one shark species (Atlantic Sharpnose) for high-throughput sequencing of the V4 region of the bacterial 16S rRNA gene. White muscle was analyzed for stable isotopes (δ<sup>13</sup>C and δ<sup>15</sup>N) to evaluate potential niche overlap in these four sympatric mesopredators. Significant differences were found in both δ<sup>13</sup>C and δ<sup>15</sup>N signatures across species, suggesting a degree of resource partitioning. When examined within tissue type, the host species had a weak effect on β-diversity for cloaca and skin, with no differences found for gill and gut samples. However, network analysis metrics demonstrated a stronger species-specific effect and distinct microbial community relationships were apparent between the shark and batoids, with the former having tighter networks for both internally- and externally-influenced tissues (gut/cloaca and skin/gills, respectively). Despite overlapping habitat use, species’ microbiomes differed in their organizational structuring that paralleled differences in stable isotope results, suggesting a mediating role of species-specific ecology on bacterial microbiomes.https://www.mdpi.com/2410-3888/9/1/34sharkstingrayecologymicrobiomenetwork analysis |
| spellingShingle | Kady Lyons Christine N. Bedore Aaron B. Carlisle Lauren Moniz Timothy L. Odom Rokeya Ahmed Stephen E. Greiman Ryan M. Freedman Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast Fishes shark stingray ecology microbiome network analysis |
| title | Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast |
| title_full | Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast |
| title_fullStr | Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast |
| title_full_unstemmed | Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast |
| title_short | Network Analysis Reveals Species-Specific Organization of Microbial Communities in Four Co-Occurring Elasmobranch Species along the Georgia Coast |
| title_sort | network analysis reveals species specific organization of microbial communities in four co occurring elasmobranch species along the georgia coast |
| topic | shark stingray ecology microbiome network analysis |
| url | https://www.mdpi.com/2410-3888/9/1/34 |
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