Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia
Foot-and-mouth disease (FMD) is an acute, highly contagious viral infection of domestic and wild cloven-hoofed animals. It is known to be endemic in Zambia, with periodic outbreaks occurring in different geographical areas of the country. This study was conducted to investigate the presence of FMD v...
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| Format: | Article |
| Language: | English |
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AOSIS
2014-04-01
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| Series: | Onderstepoort Journal of Veterinary Research |
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| Online Access: | https://ojvr.org/index.php/ojvr/article/view/721 |
| _version_ | 1818277851875508224 |
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| author | Frank Banda Christopher J. Kasanga Raphael Sallu Yona Sinkala Tingiya W. Sinkombe Misheck Mulumba Mark M. Rweyemamu Philemon N. Wambura |
| author_facet | Frank Banda Christopher J. Kasanga Raphael Sallu Yona Sinkala Tingiya W. Sinkombe Misheck Mulumba Mark M. Rweyemamu Philemon N. Wambura |
| author_sort | Frank Banda |
| collection | DOAJ |
| description | Foot-and-mouth disease (FMD) is an acute, highly contagious viral infection of domestic and wild cloven-hoofed animals. It is known to be endemic in Zambia, with periodic outbreaks occurring in different geographical areas of the country. This study was conducted to investigate the presence of FMD virus (FMDV) in reported FMD-suspected cases in cattle from the Kazungula and Mbala districts of Zambia. Sixty epithelial tissues or oesophageal-pharyngeal (OP) scrapings (probang samples) were collected from Mbala (n = 51) and Kazungula (n = 9) and examined for FMDV. The FMDV viral RNA and serotypes were examined by realtime reverse transcription polymerase chain reaction (qRT-PCR) and antigen Enzyme- linked immunosorbent assay (ELISA), respectively. Twenty-two samples (36.7%) were positive for the FMDV genome by qRT-PCR with Cycle threshold (Ct) values ranging from 13 to 31. The FMDV-positive samples from epithelial tissues showed relatively higher Ct values compared to those obtained from OP scrapings, irrespective of geographical location. Forty percent (40%; n = 4) of epithelial tissues from Mbala were serotyped into SAT 2 serotype by antigen ELISA. Kazungula samples were serotyped into SAT 1. These findings indicated that Mbala and Kazungula districts had FMD outbreaks in 2012 that were ascribed to at least FMDV serotype SAT 2 and SAT 1 field strains. Furthermore, regular interaction between buffalos from the Mosi-o Tunya Park and domestic animals from surrounding areas could contribute to the occurrence of regular FMD outbreaks in Kazungula, whilst the uncontrolled animal movements across borders between Mbala and Nsumbawanga could be responsible for disease outbreaks in Mbala. In-depth molecular biological studies, including sequencing and phylogeny of the viruses, should be conducted to elucidate the complex epidemiology of FMD in Zambia, thereby providing valuable information needed for the rational control strategy of FMD in Zambia and neighbouring countries. |
| first_indexed | 2024-12-12T23:08:07Z |
| format | Article |
| id | doaj.art-c3315c58bdeb4b868aa8f5ca03b4dc47 |
| institution | Directory Open Access Journal |
| issn | 0030-2465 2219-0635 |
| language | English |
| last_indexed | 2024-12-12T23:08:07Z |
| publishDate | 2014-04-01 |
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| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj.art-c3315c58bdeb4b868aa8f5ca03b4dc472022-12-22T00:08:39ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352014-04-01812e1e610.4102/ojvr.v81i2.721432Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of ZambiaFrank Banda0Christopher J. Kasanga1Raphael Sallu2Yona Sinkala3Tingiya W. Sinkombe4Misheck Mulumba5Mark M. Rweyemamu6Philemon N. Wambura7Southern African Centre for Infectious diseases Surveillance, Sokoine University of Agriculture, Tanzania and Central Veterinary Research Institute, ZambiaSouthern African Centre for Infectious diseases Surveillance, Sokoine University of AgricultureTanzania Veterinary Laboratory AgencyUniversity of Zambia, School of Veterinary MedicineCentral Veterinary Research InstituteSouthern African Development Community SecretariatSouthern African Centre for Infectious diseases Surveillance, Sokoine University of AgricultureSouthern African Centre for Infectious diseases Surveillance, Sokoine University of AgricultureFoot-and-mouth disease (FMD) is an acute, highly contagious viral infection of domestic and wild cloven-hoofed animals. It is known to be endemic in Zambia, with periodic outbreaks occurring in different geographical areas of the country. This study was conducted to investigate the presence of FMD virus (FMDV) in reported FMD-suspected cases in cattle from the Kazungula and Mbala districts of Zambia. Sixty epithelial tissues or oesophageal-pharyngeal (OP) scrapings (probang samples) were collected from Mbala (n = 51) and Kazungula (n = 9) and examined for FMDV. The FMDV viral RNA and serotypes were examined by realtime reverse transcription polymerase chain reaction (qRT-PCR) and antigen Enzyme- linked immunosorbent assay (ELISA), respectively. Twenty-two samples (36.7%) were positive for the FMDV genome by qRT-PCR with Cycle threshold (Ct) values ranging from 13 to 31. The FMDV-positive samples from epithelial tissues showed relatively higher Ct values compared to those obtained from OP scrapings, irrespective of geographical location. Forty percent (40%; n = 4) of epithelial tissues from Mbala were serotyped into SAT 2 serotype by antigen ELISA. Kazungula samples were serotyped into SAT 1. These findings indicated that Mbala and Kazungula districts had FMD outbreaks in 2012 that were ascribed to at least FMDV serotype SAT 2 and SAT 1 field strains. Furthermore, regular interaction between buffalos from the Mosi-o Tunya Park and domestic animals from surrounding areas could contribute to the occurrence of regular FMD outbreaks in Kazungula, whilst the uncontrolled animal movements across borders between Mbala and Nsumbawanga could be responsible for disease outbreaks in Mbala. In-depth molecular biological studies, including sequencing and phylogeny of the viruses, should be conducted to elucidate the complex epidemiology of FMD in Zambia, thereby providing valuable information needed for the rational control strategy of FMD in Zambia and neighbouring countries.https://ojvr.org/index.php/ojvr/article/view/721Foot-and-mouth disease virusserotypeantigen ELISAqRT-PCRZambia |
| spellingShingle | Frank Banda Christopher J. Kasanga Raphael Sallu Yona Sinkala Tingiya W. Sinkombe Misheck Mulumba Mark M. Rweyemamu Philemon N. Wambura Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia Onderstepoort Journal of Veterinary Research Foot-and-mouth disease virus serotype antigen ELISA qRT-PCR Zambia |
| title | Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia |
| title_full | Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia |
| title_fullStr | Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia |
| title_full_unstemmed | Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia |
| title_short | Investigation of foot-and-mouth disease outbreaks in the Mbala and Kazungula districts of Zambia |
| title_sort | investigation of foot and mouth disease outbreaks in the mbala and kazungula districts of zambia |
| topic | Foot-and-mouth disease virus serotype antigen ELISA qRT-PCR Zambia |
| url | https://ojvr.org/index.php/ojvr/article/view/721 |
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