ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group
Yewei Fang,1 Shuan Tao,2 Huimin Chen,2 Yao Xu,3 Luyan Chen,4 Wei Liang1 1Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, Ningbo, People’s Republic of China; 2School of Medicine, Jiangsu University, Zhejiang, People’s Republic of China; 3School of Medicine, Ning...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-08-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/esbl-producing-and-non-esbl-producing-escherichia-coli-isolates-from-u-peer-reviewed-fulltext-article-IDR |
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| author | Fang Y Tao S Chen H Xu Y Chen L Liang W |
| author_facet | Fang Y Tao S Chen H Xu Y Chen L Liang W |
| author_sort | Fang Y |
| collection | DOAJ |
| description | Yewei Fang,1 Shuan Tao,2 Huimin Chen,2 Yao Xu,3 Luyan Chen,4 Wei Liang1 1Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, Ningbo, People’s Republic of China; 2School of Medicine, Jiangsu University, Zhejiang, People’s Republic of China; 3School of Medicine, Ningbo University, Ningbo, People’s Republic of China; 4Department of Blood Transfusion, The First Affiliated Hospital of Ningbo University, Ningbo, People’s Republic of ChinaCorrespondence: Wei Liang, Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, No. 59. Liuting Road, Haishu District, Zhejiang, 315010, People’s Republic of China, Tel +86 15351883016, Fax +86-51885213100, Email hslwys@163.comPurpose: The objectives of this study are to determine the differences in clonality, virulence gene (VG) content and phylogenetic group between non extended-spectrum beta-lactamase-producing E. coli (non-ESBL-EC) and ESBL-EC isolates from urine.Patients and Methods: This study characterized a total of 100 clinical E. coli isolates consecutively obtained from the inpatients hospitalized in The First Affiliated Hospital of Ningbo University in China by polymerase-chain reaction (PCR).Results: Phylogenetic group B2 was found to be the most prevalent in both ESBL-EC and non-ESBL-EC group. Among 100 clinical isolates, the count of acquired virulence genes in group B2 was found to be significantly higher than that in group A, B1, and D (p < 0.001). Additionally, the presence of content within virulence genes (the total number of virulence genes detected per isolate) in B2 of non-ESBL-EC and ESBL-EC showed a significant difference (p< 0.001). ST131 was detected exclusively in ESBL-EC, while ST95 and ST73 were the main sequence types in non-ESBL-EC.Conclusion: Our study demonstrated the different distribution of MLST, phylogenetic group in ESBL-EC and non-ESBL-EC group. The inverse association between beta-lactamase resistance and VG content performed in this study should get a lot more attention. At the same time, we should also be wary of the appearance of non-ESBL-EC isolates of group B2 harboring more virulence genes which will lead to high pathogenicity.Keywords: E. coli, clonal structure, ST73, ST95, ST131, ST1193, virulence genes |
| first_indexed | 2024-03-12T13:29:49Z |
| format | Article |
| id | doaj.art-c332fd0f08834adfb14daeeb97a4da12 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-03-12T13:29:49Z |
| publishDate | 2023-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-c332fd0f08834adfb14daeeb97a4da122023-08-24T19:13:22ZengDove Medical PressInfection and Drug Resistance1178-69732023-08-01Volume 165563557186126ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic GroupFang YTao SChen HXu YChen LLiang WYewei Fang,1 Shuan Tao,2 Huimin Chen,2 Yao Xu,3 Luyan Chen,4 Wei Liang1 1Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, Ningbo, People’s Republic of China; 2School of Medicine, Jiangsu University, Zhejiang, People’s Republic of China; 3School of Medicine, Ningbo University, Ningbo, People’s Republic of China; 4Department of Blood Transfusion, The First Affiliated Hospital of Ningbo University, Ningbo, People’s Republic of ChinaCorrespondence: Wei Liang, Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, No. 59. Liuting Road, Haishu District, Zhejiang, 315010, People’s Republic of China, Tel +86 15351883016, Fax +86-51885213100, Email hslwys@163.comPurpose: The objectives of this study are to determine the differences in clonality, virulence gene (VG) content and phylogenetic group between non extended-spectrum beta-lactamase-producing E. coli (non-ESBL-EC) and ESBL-EC isolates from urine.Patients and Methods: This study characterized a total of 100 clinical E. coli isolates consecutively obtained from the inpatients hospitalized in The First Affiliated Hospital of Ningbo University in China by polymerase-chain reaction (PCR).Results: Phylogenetic group B2 was found to be the most prevalent in both ESBL-EC and non-ESBL-EC group. Among 100 clinical isolates, the count of acquired virulence genes in group B2 was found to be significantly higher than that in group A, B1, and D (p < 0.001). Additionally, the presence of content within virulence genes (the total number of virulence genes detected per isolate) in B2 of non-ESBL-EC and ESBL-EC showed a significant difference (p< 0.001). ST131 was detected exclusively in ESBL-EC, while ST95 and ST73 were the main sequence types in non-ESBL-EC.Conclusion: Our study demonstrated the different distribution of MLST, phylogenetic group in ESBL-EC and non-ESBL-EC group. The inverse association between beta-lactamase resistance and VG content performed in this study should get a lot more attention. At the same time, we should also be wary of the appearance of non-ESBL-EC isolates of group B2 harboring more virulence genes which will lead to high pathogenicity.Keywords: E. coli, clonal structure, ST73, ST95, ST131, ST1193, virulence geneshttps://www.dovepress.com/esbl-producing-and-non-esbl-producing-escherichia-coli-isolates-from-u-peer-reviewed-fulltext-article-IDRe. coliclonal structurest73st95st131st1193virulence genes |
| spellingShingle | Fang Y Tao S Chen H Xu Y Chen L Liang W ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group Infection and Drug Resistance e. coli clonal structure st73 st95 st131 st1193 virulence genes |
| title | ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group |
| title_full | ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group |
| title_fullStr | ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group |
| title_full_unstemmed | ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group |
| title_short | ESBL-Producing and Non-ESBL-Producing Escherichia coli Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group |
| title_sort | esbl producing and non esbl producing escherichia coli isolates from urinary tract differ in clonal distribution virulence gene content and phylogenetic group |
| topic | e. coli clonal structure st73 st95 st131 st1193 virulence genes |
| url | https://www.dovepress.com/esbl-producing-and-non-esbl-producing-escherichia-coli-isolates-from-u-peer-reviewed-fulltext-article-IDR |
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