Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i>
The glossiphoniid leech, <i>Helobdella austinensis</i>, is an experimentally tractable member of the superphylum, Lophotrochozoa. Its large embryonic cells, stereotyped asymmetric cell divisions and ex vivo development capabilities makes it a favorable model for studying the molecular an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/15/3/283 |
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| author | Samuel Hsaio Naim Saglam David Morrow Daniel H. Shain |
| author_facet | Samuel Hsaio Naim Saglam David Morrow Daniel H. Shain |
| author_sort | Samuel Hsaio |
| collection | DOAJ |
| description | The glossiphoniid leech, <i>Helobdella austinensis</i>, is an experimentally tractable member of the superphylum, Lophotrochozoa. Its large embryonic cells, stereotyped asymmetric cell divisions and ex vivo development capabilities makes it a favorable model for studying the molecular and cellular events of a representative spiralian. In this study, we focused on a narrow developmental time window of ~6–8 h, comprising stages just prior to and immediately following zygote deposition. Employing RNA-Seq methodology, we identified differentially expressed transcripts at this fundamental ontogenic boundary, known as the maternal-to-zygotic transition (MZT). Gene expression changes were characterized by the massive degradation of maternal RNAs (~45%) coupled with the rapid transcription of ~5000 zygotic genes (~20% of the genome) in the first mitotic cell cycle. The latter transcripts encoded a mixture of cell maintenance and regulatory proteins that predictably influence downstream developmental events. |
| first_indexed | 2024-04-24T18:15:04Z |
| format | Article |
| id | doaj.art-c33451c8b6f541acbea155e168421298 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-04-24T18:15:04Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-c33451c8b6f541acbea155e1684212982024-03-27T13:42:56ZengMDPI AGGenes2073-44252024-02-0115328310.3390/genes15030283Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i>Samuel Hsaio0Naim Saglam1David Morrow2Daniel H. Shain3Center for Computational and Integrative Biology, Rutgers The State University of New Jersey, Joint Health Sciences Center, 201 South Broadway, Camden, NJ 08103, USADepartment of Aquaculture and Fish Diseases, Fisheries Faculty, Firat University, 23200 Elazig, TürkiyeBiology Department, Rutgers The State University of New Jersey, Joint Health Sciences Center, 201 South Broadway, Camden, NJ 08103, USACenter for Computational and Integrative Biology, Rutgers The State University of New Jersey, Joint Health Sciences Center, 201 South Broadway, Camden, NJ 08103, USAThe glossiphoniid leech, <i>Helobdella austinensis</i>, is an experimentally tractable member of the superphylum, Lophotrochozoa. Its large embryonic cells, stereotyped asymmetric cell divisions and ex vivo development capabilities makes it a favorable model for studying the molecular and cellular events of a representative spiralian. In this study, we focused on a narrow developmental time window of ~6–8 h, comprising stages just prior to and immediately following zygote deposition. Employing RNA-Seq methodology, we identified differentially expressed transcripts at this fundamental ontogenic boundary, known as the maternal-to-zygotic transition (MZT). Gene expression changes were characterized by the massive degradation of maternal RNAs (~45%) coupled with the rapid transcription of ~5000 zygotic genes (~20% of the genome) in the first mitotic cell cycle. The latter transcripts encoded a mixture of cell maintenance and regulatory proteins that predictably influence downstream developmental events.https://www.mdpi.com/2073-4425/15/3/283glossiphoniiddevelopmentmaternal RNAdifferential expressionfertilization |
| spellingShingle | Samuel Hsaio Naim Saglam David Morrow Daniel H. Shain Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i> Genes glossiphoniid development maternal RNA differential expression fertilization |
| title | Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i> |
| title_full | Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i> |
| title_fullStr | Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i> |
| title_full_unstemmed | Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i> |
| title_short | Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, <i>Helobdella austinensis</i> |
| title_sort | transcriptomic profiling at the maternal to zygotic transition in leech i helobdella austinensis i |
| topic | glossiphoniid development maternal RNA differential expression fertilization |
| url | https://www.mdpi.com/2073-4425/15/3/283 |
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