A rat model of dual-flow liver machine perfusion system
ABSTRACT Purpose: As clinical liver perfusion systems use portal vein and artery flow, dual perfusion techniques are required even in small animal models in order to reproduce clinical setting. The aim of this study was to construct a new dual-flow perfusion system in rat model and optimized the ox...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2023-10-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502023000100245&lng=en&tlng=en |
| _version_ | 1827778329351028736 |
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| author | Masayuki Ohara Jun Ishikawa Syuhei Yoshimoto Yoji Hakamata Eiji Kobayashi |
| author_facet | Masayuki Ohara Jun Ishikawa Syuhei Yoshimoto Yoji Hakamata Eiji Kobayashi |
| author_sort | Masayuki Ohara |
| collection | DOAJ |
| description | ABSTRACT Purpose: As clinical liver perfusion systems use portal vein and artery flow, dual perfusion techniques are required even in small animal models in order to reproduce clinical setting. The aim of this study was to construct a new dual-flow perfusion system in rat model and optimized the oxygen supply to ensure the aerobic metabolization. Methods: The dual-flow circuit was fabricated using rat liver and whole blood samples as perfusates. The oxygen supply was controlled according to the amount of dissolved oxygen in the perfusate. Perfusate parameters and adenosine triphosphate (ATP) levels were analyzed to evaluate organ function and metabolic energy state. Stored whole blood also tested the suitability as perfusate. Results: Stored blood showed decrease oxygen delivery and liver function compared to fresh blood. Using fresh blood as perfusate with air only, the dissolved oxygen levels remained low and anaerobic metabolism increased. In contrast, with oxygen control at living body level, anaerobic metabolism was well suppressed, and tissue ATP content was increased. Conclusions: We developed a new dual-flow system that enable to reproduce the clinical settings. The perfusion system showed the possibility to improve the energy metabolic state of the perfused organ under appropriate partial pressure of oxygen. |
| first_indexed | 2024-03-11T14:31:29Z |
| format | Article |
| id | doaj.art-c334d908a8be4277bee2b72d9616f27e |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-03-11T14:31:29Z |
| publishDate | 2023-10-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-c334d908a8be4277bee2b72d9616f27e2023-10-31T07:53:56ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742023-10-013810.1590/acb387723A rat model of dual-flow liver machine perfusion systemMasayuki Oharahttps://orcid.org/0009-0007-8681-0432Jun Ishikawahttps://orcid.org/0000-0002-3430-7479Syuhei Yoshimotohttps://orcid.org/0000-0003-0246-483XYoji Hakamatahttps://orcid.org/0000-0002-0138-6692Eiji Kobayashihttps://orcid.org/0000-0001-8617-3778ABSTRACT Purpose: As clinical liver perfusion systems use portal vein and artery flow, dual perfusion techniques are required even in small animal models in order to reproduce clinical setting. The aim of this study was to construct a new dual-flow perfusion system in rat model and optimized the oxygen supply to ensure the aerobic metabolization. Methods: The dual-flow circuit was fabricated using rat liver and whole blood samples as perfusates. The oxygen supply was controlled according to the amount of dissolved oxygen in the perfusate. Perfusate parameters and adenosine triphosphate (ATP) levels were analyzed to evaluate organ function and metabolic energy state. Stored whole blood also tested the suitability as perfusate. Results: Stored blood showed decrease oxygen delivery and liver function compared to fresh blood. Using fresh blood as perfusate with air only, the dissolved oxygen levels remained low and anaerobic metabolism increased. In contrast, with oxygen control at living body level, anaerobic metabolism was well suppressed, and tissue ATP content was increased. Conclusions: We developed a new dual-flow system that enable to reproduce the clinical settings. The perfusion system showed the possibility to improve the energy metabolic state of the perfused organ under appropriate partial pressure of oxygen.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502023000100245&lng=en&tlng=enOxygenationPerfusionRatsIschemiaLiver |
| spellingShingle | Masayuki Ohara Jun Ishikawa Syuhei Yoshimoto Yoji Hakamata Eiji Kobayashi A rat model of dual-flow liver machine perfusion system Acta Cirúrgica Brasileira Oxygenation Perfusion Rats Ischemia Liver |
| title | A rat model of dual-flow liver machine perfusion system |
| title_full | A rat model of dual-flow liver machine perfusion system |
| title_fullStr | A rat model of dual-flow liver machine perfusion system |
| title_full_unstemmed | A rat model of dual-flow liver machine perfusion system |
| title_short | A rat model of dual-flow liver machine perfusion system |
| title_sort | rat model of dual flow liver machine perfusion system |
| topic | Oxygenation Perfusion Rats Ischemia Liver |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502023000100245&lng=en&tlng=en |
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