Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â -converting enzyme in vitro
Objective: To explore the possible inhibitory potentials and mechanism by Mobola plum (Parinari curatellifolia) seeds crude methanol (CE) and flavonoid-rich (FE) extracts on angiotensin-1-converting enzyme (ACE â ). Methods: The sensitivity and kinetic model of inhibition of CE and FE on ACE â us...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2017-12-01
|
Series: | Asian Pacific Journal of Tropical Biomedicine |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169117309577 |
_version_ | 1811268255581470720 |
---|---|
author | Olamide Olajusi Crown Olanrewaju Sam Olayeriju Ayodele Oluwaseyi Kolawole Afolabi Clement Akinmoladun Mary Tolulope Olaleye Afolabi Akintunde Akindahunsi |
author_facet | Olamide Olajusi Crown Olanrewaju Sam Olayeriju Ayodele Oluwaseyi Kolawole Afolabi Clement Akinmoladun Mary Tolulope Olaleye Afolabi Akintunde Akindahunsi |
author_sort | Olamide Olajusi Crown |
collection | DOAJ |
description | Objective: To explore the possible inhibitory potentials and mechanism by Mobola plum (Parinari curatellifolia) seeds crude methanol (CE) and flavonoid-rich (FE) extracts on angiotensin-1-converting enzyme (ACE â
). Methods: The sensitivity and kinetic model of inhibition of CE and FE on ACE â
using N-[3-(2-furyl)-acryloyl]-Phe-Gly-Gly as enzyme substrate for ACE â
was evaluated by Michealis Menten approach. The inhibition mechanism was explored from LineweaverâBurk model and IC50 was determined from ChengâPrusoff empirical analysis. Results: The IC50 of CE and FE were 13.54 and 39.38 μg/mL, respectively. Both extracts exhibited mixed type inhibition with the inhibitory constant (Ki) of CE was between 0.38 and 0.37 μg/mL while that of FE showed a two-fold increase (1.62 μg/mL and 0.28 μg/mL). FE on ACE â
demonstrated positive cooperativity with a Hill's coefficient of 1.89. Conclusions: The study reveals the superior ACE â
inhibitory potential of CE over FE and suggest that mixed inhibition pattern of the enzyme might be the underlying mechanism of antihypertensive activity. Keywords: Angiotensin converting enzyme, Blood pressure, FAPGG, Mixed type inhibition, Parinari curatellifolia |
first_indexed | 2024-04-12T21:19:35Z |
format | Article |
id | doaj.art-c33636fd54ac44c2adc4f4de4464ec9e |
institution | Directory Open Access Journal |
issn | 2221-1691 |
language | English |
last_indexed | 2024-04-12T21:19:35Z |
publishDate | 2017-12-01 |
publisher | Wolters Kluwer Medknow Publications |
record_format | Article |
series | Asian Pacific Journal of Tropical Biomedicine |
spelling | doaj.art-c33636fd54ac44c2adc4f4de4464ec9e2022-12-22T03:16:21ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-12-0171210791084Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â
-converting enzyme in vitroOlamide Olajusi Crown0Olanrewaju Sam Olayeriju1Ayodele Oluwaseyi Kolawole2Afolabi Clement Akinmoladun3Mary Tolulope Olaleye4Afolabi Akintunde Akindahunsi5Corresponding author: Olamide Olajusi Crown, Department of Biochemistry, the Federal University of Technology, Akure, Nigeria. Tel: +234 8035379014.; Department of Biochemistry, the Federal University of Technology, Akure, NigeriaDepartment of Biochemistry, the Federal University of Technology, Akure, NigeriaDepartment of Biochemistry, the Federal University of Technology, Akure, NigeriaDepartment of Biochemistry, the Federal University of Technology, Akure, NigeriaDepartment of Biochemistry, the Federal University of Technology, Akure, NigeriaDepartment of Biochemistry, the Federal University of Technology, Akure, NigeriaObjective: To explore the possible inhibitory potentials and mechanism by Mobola plum (Parinari curatellifolia) seeds crude methanol (CE) and flavonoid-rich (FE) extracts on angiotensin-1-converting enzyme (ACE â
). Methods: The sensitivity and kinetic model of inhibition of CE and FE on ACE â
using N-[3-(2-furyl)-acryloyl]-Phe-Gly-Gly as enzyme substrate for ACE â
was evaluated by Michealis Menten approach. The inhibition mechanism was explored from LineweaverâBurk model and IC50 was determined from ChengâPrusoff empirical analysis. Results: The IC50 of CE and FE were 13.54 and 39.38 μg/mL, respectively. Both extracts exhibited mixed type inhibition with the inhibitory constant (Ki) of CE was between 0.38 and 0.37 μg/mL while that of FE showed a two-fold increase (1.62 μg/mL and 0.28 μg/mL). FE on ACE â
demonstrated positive cooperativity with a Hill's coefficient of 1.89. Conclusions: The study reveals the superior ACE â
inhibitory potential of CE over FE and suggest that mixed inhibition pattern of the enzyme might be the underlying mechanism of antihypertensive activity. Keywords: Angiotensin converting enzyme, Blood pressure, FAPGG, Mixed type inhibition, Parinari curatellifoliahttp://www.sciencedirect.com/science/article/pii/S2221169117309577 |
spellingShingle | Olamide Olajusi Crown Olanrewaju Sam Olayeriju Ayodele Oluwaseyi Kolawole Afolabi Clement Akinmoladun Mary Tolulope Olaleye Afolabi Akintunde Akindahunsi Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â -converting enzyme in vitro Asian Pacific Journal of Tropical Biomedicine |
title | Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â
-converting enzyme in vitro |
title_full | Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â
-converting enzyme in vitro |
title_fullStr | Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â
-converting enzyme in vitro |
title_full_unstemmed | Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â
-converting enzyme in vitro |
title_short | Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin â
-converting enzyme in vitro |
title_sort | mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensin a
converting enzyme ina vitro |
url | http://www.sciencedirect.com/science/article/pii/S2221169117309577 |
work_keys_str_mv | AT olamideolajusicrown mobolaplumseedmethanolicextractsexhibitmixedtypeinhibitionofangiotensinaconvertingenzymeinavitro AT olanrewajusamolayeriju mobolaplumseedmethanolicextractsexhibitmixedtypeinhibitionofangiotensinaconvertingenzymeinavitro AT ayodeleoluwaseyikolawole mobolaplumseedmethanolicextractsexhibitmixedtypeinhibitionofangiotensinaconvertingenzymeinavitro AT afolabiclementakinmoladun mobolaplumseedmethanolicextractsexhibitmixedtypeinhibitionofangiotensinaconvertingenzymeinavitro AT marytolulopeolaleye mobolaplumseedmethanolicextractsexhibitmixedtypeinhibitionofangiotensinaconvertingenzymeinavitro AT afolabiakintundeakindahunsi mobolaplumseedmethanolicextractsexhibitmixedtypeinhibitionofangiotensinaconvertingenzymeinavitro |