3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism
Summary: Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorfer...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222021113 |
| _version_ | 1797946185408839680 |
|---|---|
| author | Yvonne Adams Anne Skovsbo Clausen Peter Østrup Jensen Malin Lager Peter Wilhelmsson Anna J. Henningson Per-Eric Lindgren Daniel Faurholt-Jepsen Helene Mens Peter Kraiczy Kasper Nørskov Kragh Thomas Bjarnsholt Andreas Kjaer Anne-Mette Lebech Anja R. Jensen |
| author_facet | Yvonne Adams Anne Skovsbo Clausen Peter Østrup Jensen Malin Lager Peter Wilhelmsson Anna J. Henningson Per-Eric Lindgren Daniel Faurholt-Jepsen Helene Mens Peter Kraiczy Kasper Nørskov Kragh Thomas Bjarnsholt Andreas Kjaer Anne-Mette Lebech Anja R. Jensen |
| author_sort | Yvonne Adams |
| collection | DOAJ |
| description | Summary: Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection. |
| first_indexed | 2024-04-10T21:06:53Z |
| format | Article |
| id | doaj.art-c337888d88e645838d653b41c3cef6d6 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:06:53Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c337888d88e645838d653b41c3cef6d62023-01-22T04:41:24ZengElsevieriScience2589-00422023-01-012611058383D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropismYvonne Adams0Anne Skovsbo Clausen1Peter Østrup Jensen2Malin Lager3Peter Wilhelmsson4Anna J. Henningson5Per-Eric Lindgren6Daniel Faurholt-Jepsen7Helene Mens8Peter Kraiczy9Kasper Nørskov Kragh10Thomas Bjarnsholt11Andreas Kjaer12Anne-Mette Lebech13Anja R. Jensen14Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Maersk Tower, Blegdamsvej 3B, 2200 Copenhagen N, Denmark; Corresponding authorDepartment of Clinical Physiology and Nuclear Medicine & Cluster for Molecular Imaging, Copenhagen University Hospital-Rigshospitalet, Copenhagen, DenmarkDepartment of Biomedical Sciences, University of Copenhagen, University Hospital-Rigshospitalet, Copenhagen, Denmark; Department of Clinical Microbiology, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark; Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkNational Reference Laboratory for Borrelia and Other Tick-Borne Bacteria, Division of Clinical Microbiology, Laboratory Medicine, Region Jönköping County and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, SwedenNational Reference Laboratory for Borrelia and Other Tick-Borne Bacteria, Division of Clinical Microbiology, Laboratory Medicine, Region Jönköping County and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden; Division of Inflammation and Infection, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, SwedenNational Reference Laboratory for Borrelia and Other Tick-Borne Bacteria, Division of Clinical Microbiology, Laboratory Medicine, Region Jönköping County and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden; Division of Inflammation and Infection, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, SwedenNational Reference Laboratory for Borrelia and Other Tick-Borne Bacteria, Division of Clinical Microbiology, Laboratory Medicine, Region Jönköping County and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden; Division of Inflammation and Infection, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, SwedenDepartment of Infectious Diseases, Copenhagen University Hospital-Rigshospitalet, Copenhagen, DenmarkDepartment of Infectious Diseases, Copenhagen University Hospital-Rigshospitalet, Copenhagen, DenmarkInstitute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Goethe University Frankfurt, Frankfurt, GermanyDepartment of Clinical Microbiology, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark; Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Clinical Microbiology, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark; Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Clinical Physiology and Nuclear Medicine & Cluster for Molecular Imaging, Copenhagen University Hospital-Rigshospitalet, Copenhagen, DenmarkDepartment of Infectious Diseases, Copenhagen University Hospital-Rigshospitalet, Copenhagen, Denmark; Department of Clinical Medicine, University of Copenhagen, Copenhagen, DenmarkCentre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Maersk Tower, Blegdamsvej 3B, 2200 Copenhagen N, Denmark; Corresponding authorSummary: Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection.http://www.sciencedirect.com/science/article/pii/S2589004222021113NeuroscienceMedical MicrobiologyCell biology |
| spellingShingle | Yvonne Adams Anne Skovsbo Clausen Peter Østrup Jensen Malin Lager Peter Wilhelmsson Anna J. Henningson Per-Eric Lindgren Daniel Faurholt-Jepsen Helene Mens Peter Kraiczy Kasper Nørskov Kragh Thomas Bjarnsholt Andreas Kjaer Anne-Mette Lebech Anja R. Jensen 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism iScience Neuroscience Medical Microbiology Cell biology |
| title | 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism |
| title_full | 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism |
| title_fullStr | 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism |
| title_full_unstemmed | 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism |
| title_short | 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism |
| title_sort | 3d blood brain barrier organoids as a model for lyme neuroborreliosis highlighting genospecies dependent organotropism |
| topic | Neuroscience Medical Microbiology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222021113 |
| work_keys_str_mv | AT yvonneadams 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT anneskovsboclausen 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT peterøstrupjensen 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT malinlager 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT peterwilhelmsson 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT annajhenningson 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT perericlindgren 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT danielfaurholtjepsen 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT helenemens 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT peterkraiczy 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT kaspernørskovkragh 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT thomasbjarnsholt 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT andreaskjaer 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT annemettelebech 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism AT anjarjensen 3dbloodbrainbarrierorganoidsasamodelforlymeneuroborreliosishighlightinggenospeciesdependentorganotropism |