Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns
The intestinal microbiota, a barrier to the establishment of pathogenic bacteria, is also an important reservoir of opportunistic pathogens. It plays a key role in the process of resistance-genes dissemination, commonly carried by specialized genetic elements, like plasmids, phages, and conjugative...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1999-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000600016 |
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| author | Julio César de Siqueira Barros Sebastião Ronaldo Pinheiro Marcelo Bozza Frederico José Gueiros-Filho Alexandre Ribeiro Bello Ulisses Gazos Lopes José Augusto Adler Pereira |
| author_facet | Julio César de Siqueira Barros Sebastião Ronaldo Pinheiro Marcelo Bozza Frederico José Gueiros-Filho Alexandre Ribeiro Bello Ulisses Gazos Lopes José Augusto Adler Pereira |
| author_sort | Julio César de Siqueira Barros |
| collection | DOAJ |
| description | The intestinal microbiota, a barrier to the establishment of pathogenic bacteria, is also an important reservoir of opportunistic pathogens. It plays a key role in the process of resistance-genes dissemination, commonly carried by specialized genetic elements, like plasmids, phages, and conjugative transposons. We obtained from strains of enterobacteria, isolated from faeces of newborns in a university hospital nursery, indication of phenothypical gentamicin resistance amplification (frequencies of 10-3 to 10-5, compatible with transposition frequencies). Southern blotting assays showed strong hybridization signals for both plasmidial and chromossomal regions in DNA extracted from variants selected at high gentamicin concentrations, using as a probe a labeled cloned insert containing aminoglycoside modifying enzyme (AME) gene sequence originated from a plasmid of a Klebsiella pneumoniae strain previously isolated in the same hospital. Further, we found indications of inactivation to other resistance genes in variants selected under similar conditions, as well as, indications of co-amplification of other AME markers (amikacin). Since the intestinal environment is a scenario of selective processes due to the therapeutic and prophylactic use of antimicrobial agents, the processes of amplification of low level antimicrobial resistance (not usually detected or sought by common methods used for antibiotic resistance surveillance) might compromise the effectiveness of antibiotic chemotherapy. |
| first_indexed | 2024-03-12T07:23:35Z |
| format | Article |
| id | doaj.art-c339145cef35406e9a8f6fb5893de8a0 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:23:35Z |
| publishDate | 1999-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c339145cef35406e9a8f6fb5893de8a02023-09-02T22:15:02ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601999-11-0194679580210.1590/S0074-02761999000600016Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newbornsJulio César de Siqueira BarrosSebastião Ronaldo PinheiroMarcelo BozzaFrederico José Gueiros-FilhoAlexandre Ribeiro BelloUlisses Gazos LopesJosé Augusto Adler PereiraThe intestinal microbiota, a barrier to the establishment of pathogenic bacteria, is also an important reservoir of opportunistic pathogens. It plays a key role in the process of resistance-genes dissemination, commonly carried by specialized genetic elements, like plasmids, phages, and conjugative transposons. We obtained from strains of enterobacteria, isolated from faeces of newborns in a university hospital nursery, indication of phenothypical gentamicin resistance amplification (frequencies of 10-3 to 10-5, compatible with transposition frequencies). Southern blotting assays showed strong hybridization signals for both plasmidial and chromossomal regions in DNA extracted from variants selected at high gentamicin concentrations, using as a probe a labeled cloned insert containing aminoglycoside modifying enzyme (AME) gene sequence originated from a plasmid of a Klebsiella pneumoniae strain previously isolated in the same hospital. Further, we found indications of inactivation to other resistance genes in variants selected under similar conditions, as well as, indications of co-amplification of other AME markers (amikacin). Since the intestinal environment is a scenario of selective processes due to the therapeutic and prophylactic use of antimicrobial agents, the processes of amplification of low level antimicrobial resistance (not usually detected or sought by common methods used for antibiotic resistance surveillance) might compromise the effectiveness of antibiotic chemotherapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000600016resistance amplificationaminoglycosides modifying enzymesKlebsiella pneumoniae |
| spellingShingle | Julio César de Siqueira Barros Sebastião Ronaldo Pinheiro Marcelo Bozza Frederico José Gueiros-Filho Alexandre Ribeiro Bello Ulisses Gazos Lopes José Augusto Adler Pereira Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns Memorias do Instituto Oswaldo Cruz resistance amplification aminoglycosides modifying enzymes Klebsiella pneumoniae |
| title | Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns |
| title_full | Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns |
| title_fullStr | Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns |
| title_full_unstemmed | Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns |
| title_short | Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns |
| title_sort | evidences of gentamicin resistance amplification in klebsiella pneumoniae isolated from faeces of hospitalized newborns |
| topic | resistance amplification aminoglycosides modifying enzymes Klebsiella pneumoniae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000600016 |
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