The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14
Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI...
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02312/full |
| _version_ | 1811220236119048192 |
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| author | Muralidhar Tata Fabian Amman Vinay Pawar Vinay Pawar Michael T. Wolfinger Siegfried Weiss Susanne Häussler Susanne Häussler Udo Bläsi |
| author_facet | Muralidhar Tata Fabian Amman Vinay Pawar Vinay Pawar Michael T. Wolfinger Siegfried Weiss Susanne Häussler Susanne Häussler Udo Bläsi |
| author_sort | Muralidhar Tata |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI is anaerobically induced in the presence of nitrate and depends on the two-component system NarXL. Our studies revealed that PaiI is required for efficient denitrification affecting the conversion of nitrite to nitric oxide. In the absence of PaiI anaerobic growth was impaired on glucose, which can be reconciled with a decreased uptake of the carbon source under these conditions. The importance of PaiI for anaerobic growth is further underlined by the observation that a paiI deletion mutant was impaired in growth in murine tumors. |
| first_indexed | 2024-04-12T07:39:18Z |
| format | Article |
| id | doaj.art-c33a12605ab649958e2ab7a875e8e6b8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T07:39:18Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c33a12605ab649958e2ab7a875e8e6b82022-12-22T03:41:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-11-01810.3389/fmicb.2017.02312318957The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14Muralidhar Tata0Fabian Amman1Vinay Pawar2Vinay Pawar3Michael T. Wolfinger4Siegfried Weiss5Susanne Häussler6Susanne Häussler7Udo Bläsi8Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Vienna, AustriaInstitute of Theoretical Chemistry, University of Vienna, Vienna, AustriaInstitute of Immunology, Hannover Medical School, Hannover, GermanyDepartment of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, GermanyInstitute of Theoretical Chemistry, University of Vienna, Vienna, AustriaInstitute of Immunology, Hannover Medical School, Hannover, GermanyDepartment of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, GermanyInstitute of Molecular Bacteriology, Twincore, Center for Experimental and Clinical Infection Research, Hannover, GermanyDepartment of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Vienna, AustriaPseudomonas aeruginosa is an opportunistic pathogen that can thrive by anaerobic respiration in the lungs of cystic fibrosis patients using nitrate as terminal electron acceptor. Here, we report the identification and characterization of the small RNA PaiI in the P. aeruginosa strain 14 (PA14). PaiI is anaerobically induced in the presence of nitrate and depends on the two-component system NarXL. Our studies revealed that PaiI is required for efficient denitrification affecting the conversion of nitrite to nitric oxide. In the absence of PaiI anaerobic growth was impaired on glucose, which can be reconciled with a decreased uptake of the carbon source under these conditions. The importance of PaiI for anaerobic growth is further underlined by the observation that a paiI deletion mutant was impaired in growth in murine tumors.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02312/fullPseudomonas aeruginosaanaerobiosissmall RNAdenitrificationnitrite |
| spellingShingle | Muralidhar Tata Fabian Amman Vinay Pawar Vinay Pawar Michael T. Wolfinger Siegfried Weiss Susanne Häussler Susanne Häussler Udo Bläsi The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 Frontiers in Microbiology Pseudomonas aeruginosa anaerobiosis small RNA denitrification nitrite |
| title | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
| title_full | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
| title_fullStr | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
| title_full_unstemmed | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
| title_short | The Anaerobically Induced sRNA PaiI Affects Denitrification in Pseudomonas aeruginosa PA14 |
| title_sort | anaerobically induced srna paii affects denitrification in pseudomonas aeruginosa pa14 |
| topic | Pseudomonas aeruginosa anaerobiosis small RNA denitrification nitrite |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02312/full |
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