Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i>
The olfactory system in insects are crucial for recognition of host plants and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in detecting odorants released by host plants. <i>Orthaga achatina</i> (Lepidoptera: Pyralidae) is one of the most serious...
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MDPI AG
2023-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/3/216 |
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| author | Yu Ma Yu Li Zhi-Qiang Wei Jing-Hao Hou Yu-Xiao Si Jin Zhang Shuang-Lin Dong Qi Yan |
| author_facet | Yu Ma Yu Li Zhi-Qiang Wei Jing-Hao Hou Yu-Xiao Si Jin Zhang Shuang-Lin Dong Qi Yan |
| author_sort | Yu Ma |
| collection | DOAJ |
| description | The olfactory system in insects are crucial for recognition of host plants and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in detecting odorants released by host plants. <i>Orthaga achatina</i> (Lepidoptera: Pyralidae) is one of the most serious pests of camphor trees, <i>Cinnamomum camphora</i> (L.) Presl, an important urban tree species in southern China. In this study, we study the GOBPs of <i>O. achatina</i>. Firstly, two full-length GOBP genes (<i>OachGOBP1</i> and <i>OachGOBP2</i>) were successfully cloned according to transcriptome sequencing results, and real-time quantitative PCR measurements showed that both GOBP genes were specifically expressed in the antennae of both sexes, proposing their important roles in olfaction. Then, both GOBP genes were heterologous expressed in <i>Escherichia coli</i> and fluorescence competitive binding assays were conducted. The results showed that OachGOBP1 could bind Farnesol (<i>K</i><sub>i</sub> = 9.49 μM) and <i>Z</i>11-16: OH (<i>K</i><sub>i</sub> = 1.57 μM). OachGOBP2 has a high binding affinity with two camphor plant volatiles (Farnesol, <i>K</i><sub>i</sub> = 7.33 μM; α-Phellandrene, <i>K</i><sub>i</sub> = 8.71 μM) and two sex pheromone components (Z11-16: OAc, <i>K</i><sub>i</sub> = 2.84 μM; Z11-16: OH, <i>K</i><sub>i</sub> = 3.30 μM). These results indicate that OachGOBP1 and OachGOBP2 differ in terms of odorants and other ligands. Furthermore, key amino acid residues that bind to plant volatiles were identified in GOBPs using 3-D structure modeling and ligand molecular docking, predicting the interactions between the GOBPs and the host plant volatiles. |
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| issn | 2075-4450 |
| language | English |
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| spelling | doaj.art-c33c31b8fde64fe8923eb6a105121bf62023-11-17T11:45:31ZengMDPI AGInsects2075-44502023-02-0114321610.3390/insects14030216Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i>Yu Ma0Yu Li1Zhi-Qiang Wei2Jing-Hao Hou3Yu-Xiao Si4Jin Zhang5Shuang-Lin Dong6Qi Yan7Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, ChinaThe olfactory system in insects are crucial for recognition of host plants and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in detecting odorants released by host plants. <i>Orthaga achatina</i> (Lepidoptera: Pyralidae) is one of the most serious pests of camphor trees, <i>Cinnamomum camphora</i> (L.) Presl, an important urban tree species in southern China. In this study, we study the GOBPs of <i>O. achatina</i>. Firstly, two full-length GOBP genes (<i>OachGOBP1</i> and <i>OachGOBP2</i>) were successfully cloned according to transcriptome sequencing results, and real-time quantitative PCR measurements showed that both GOBP genes were specifically expressed in the antennae of both sexes, proposing their important roles in olfaction. Then, both GOBP genes were heterologous expressed in <i>Escherichia coli</i> and fluorescence competitive binding assays were conducted. The results showed that OachGOBP1 could bind Farnesol (<i>K</i><sub>i</sub> = 9.49 μM) and <i>Z</i>11-16: OH (<i>K</i><sub>i</sub> = 1.57 μM). OachGOBP2 has a high binding affinity with two camphor plant volatiles (Farnesol, <i>K</i><sub>i</sub> = 7.33 μM; α-Phellandrene, <i>K</i><sub>i</sub> = 8.71 μM) and two sex pheromone components (Z11-16: OAc, <i>K</i><sub>i</sub> = 2.84 μM; Z11-16: OH, <i>K</i><sub>i</sub> = 3.30 μM). These results indicate that OachGOBP1 and OachGOBP2 differ in terms of odorants and other ligands. Furthermore, key amino acid residues that bind to plant volatiles were identified in GOBPs using 3-D structure modeling and ligand molecular docking, predicting the interactions between the GOBPs and the host plant volatiles.https://www.mdpi.com/2075-4450/14/3/216<i>Orthaga achiatina</i>general odorant binding proteinfluorescence competitive binding assaymolecular docking |
| spellingShingle | Yu Ma Yu Li Zhi-Qiang Wei Jing-Hao Hou Yu-Xiao Si Jin Zhang Shuang-Lin Dong Qi Yan Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i> Insects <i>Orthaga achiatina</i> general odorant binding protein fluorescence competitive binding assay molecular docking |
| title | Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i> |
| title_full | Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i> |
| title_fullStr | Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i> |
| title_full_unstemmed | Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i> |
| title_short | Identification and Functional Characterization of General Odorant Binding Proteins in <i>Orthaga achatina</i> |
| title_sort | identification and functional characterization of general odorant binding proteins in i orthaga achatina i |
| topic | <i>Orthaga achiatina</i> general odorant binding protein fluorescence competitive binding assay molecular docking |
| url | https://www.mdpi.com/2075-4450/14/3/216 |
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