Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica
Scouting for novel and plant-derived biomolecules from endophytic microbial sources draws greater focus on the discovery of novel bioactive metabolites. With this rationale, we scouted the endophytic fungi for taxol, an anticancer diterpenoid and fluorescent biomolecules. In the present study, about...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2021-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-of-taxol-and-flavin-like-fluorochrome-from-endophytic-fungi-of-mangifera-indica/ |
| _version_ | 1818383620738383872 |
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| author | Enthai Ganeshan Jagan Parth Sharma Senthuran Sureshkumar Mohan Pandi |
| author_facet | Enthai Ganeshan Jagan Parth Sharma Senthuran Sureshkumar Mohan Pandi |
| author_sort | Enthai Ganeshan Jagan |
| collection | DOAJ |
| description | Scouting for novel and plant-derived biomolecules from endophytic microbial sources draws greater focus on the discovery of novel bioactive metabolites. With this rationale, we scouted the endophytic fungi for taxol, an anticancer diterpenoid and fluorescent biomolecules. In the present study, about 31 endophytic fungal isolates recovered from the Mangifera indica leaves were screened for taxol production in M1D medium. About five isolates were shortlisted based on the thin layer chromatographic analysis of the fungal extracts. Among them Colletotrichum sp. MIP-5 has been identified as a producer of fungal taxol based on UV, FTIR, TLC and HPLC analysis. The partially purified fungal taxol showed similar spectral and chromatographic features of commercially available paclitaxel. In addition to this, we also report the production of a fluorescent compound by Penicillium sp. MIP-3. The Flavin-like compound exhibited a bright greenish-yellow fluorescence with an emission maximum in the range of 505 – 545nm. GC-MS analysis showed the occurrence of Latia luciferin, primarily associated with the bioluminescence of freshwater limpet Latia neritoides. This is the first report of this compound from Penicillium sp. In addition, therapeutically active steroid (β-Sitosterol, Stigmasterol, Campesterol), quinones (Benzo[h]quinoline, 2,4-dimethyl-) and phloroglucinol (Aspidinol) derivatives were also identified from Penicillium sp. MIP-3 based on GC-MS analysis. These molecules could potentially be used in biological and pharmaceutical applications in future. |
| first_indexed | 2024-12-14T03:09:16Z |
| format | Article |
| id | doaj.art-c3407fdb55c644fdab00ebdc98482ca1 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-14T03:09:16Z |
| publishDate | 2021-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-c3407fdb55c644fdab00ebdc98482ca12022-12-21T23:19:19ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2021-12-011542195220810.22207/JPAM.15.4.43Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indicaEnthai Ganeshan Jaganhttps://orcid.org/0000-0003-1057-6145Parth Sharmahttps://orcid.org/0000-0003-4463-9677Senthuran Sureshkumarhttps://orcid.org/0000-0002-9859-2248Mohan Pandihttps://orcid.org/0000-0001-7604-7364Scouting for novel and plant-derived biomolecules from endophytic microbial sources draws greater focus on the discovery of novel bioactive metabolites. With this rationale, we scouted the endophytic fungi for taxol, an anticancer diterpenoid and fluorescent biomolecules. In the present study, about 31 endophytic fungal isolates recovered from the Mangifera indica leaves were screened for taxol production in M1D medium. About five isolates were shortlisted based on the thin layer chromatographic analysis of the fungal extracts. Among them Colletotrichum sp. MIP-5 has been identified as a producer of fungal taxol based on UV, FTIR, TLC and HPLC analysis. The partially purified fungal taxol showed similar spectral and chromatographic features of commercially available paclitaxel. In addition to this, we also report the production of a fluorescent compound by Penicillium sp. MIP-3. The Flavin-like compound exhibited a bright greenish-yellow fluorescence with an emission maximum in the range of 505 – 545nm. GC-MS analysis showed the occurrence of Latia luciferin, primarily associated with the bioluminescence of freshwater limpet Latia neritoides. This is the first report of this compound from Penicillium sp. In addition, therapeutically active steroid (β-Sitosterol, Stigmasterol, Campesterol), quinones (Benzo[h]quinoline, 2,4-dimethyl-) and phloroglucinol (Aspidinol) derivatives were also identified from Penicillium sp. MIP-3 based on GC-MS analysis. These molecules could potentially be used in biological and pharmaceutical applications in future.https://microbiologyjournal.org/isolation-of-taxol-and-flavin-like-fluorochrome-from-endophytic-fungi-of-mangifera-indica/bioprospectingtaxolluciferinfluorescenceanticancer |
| spellingShingle | Enthai Ganeshan Jagan Parth Sharma Senthuran Sureshkumar Mohan Pandi Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica Journal of Pure and Applied Microbiology bioprospecting taxol luciferin fluorescence anticancer |
| title | Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica |
| title_full | Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica |
| title_fullStr | Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica |
| title_full_unstemmed | Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica |
| title_short | Isolation of Taxol and Flavin-like fluorochrome from Endophytic Fungi of Mangifera indica |
| title_sort | isolation of taxol and flavin like fluorochrome from endophytic fungi of mangifera indica |
| topic | bioprospecting taxol luciferin fluorescence anticancer |
| url | https://microbiologyjournal.org/isolation-of-taxol-and-flavin-like-fluorochrome-from-endophytic-fungi-of-mangifera-indica/ |
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