Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry
Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluoresc...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/3/2/45 |
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| author | Gabriela S. Yánez-Jácome David Romero-Estévez Hugo Navarrete Karina Simbaña-Farinango Pamela Y Vélez-Terreros |
| author_facet | Gabriela S. Yánez-Jácome David Romero-Estévez Hugo Navarrete Karina Simbaña-Farinango Pamela Y Vélez-Terreros |
| author_sort | Gabriela S. Yánez-Jácome |
| collection | DOAJ |
| description | Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm<sup>3</sup> HNO<sub>3</sub>, 1 cm<sup>3</sup> H<sub>2</sub>O<sub>2</sub>, and 1 cm<sup>3</sup> HClO<sub>4</sub>), an irradiation temperature of 210 °C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg<sup>−1</sup>) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (<i>Oreochromis niloticus</i>), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 µg·dm<sup>−3</sup> and 0.394 µg·dm<sup>−3</sup>, respectively. |
| first_indexed | 2024-03-10T18:57:39Z |
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| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-10T18:57:39Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-c34400761d6a44da87b784024b41deb52023-11-20T04:39:15ZengMDPI AGMethods and Protocols2409-92792020-06-01324510.3390/mps3020045Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence SpectrophotometryGabriela S. Yánez-Jácome0David Romero-Estévez1Hugo Navarrete2Karina Simbaña-Farinango3Pamela Y Vélez-Terreros4Centro de Estudios Aplicados en Química, Pontificia Universidad Católica del Ecuador, Quito 17012184, EcuadorCentro de Estudios Aplicados en Química, Pontificia Universidad Católica del Ecuador, Quito 17012184, EcuadorCentro de Estudios Aplicados en Química, Pontificia Universidad Católica del Ecuador, Quito 17012184, EcuadorCentro de Estudios Aplicados en Química, Pontificia Universidad Católica del Ecuador, Quito 17012184, EcuadorCentro de Estudios Aplicados en Química, Pontificia Universidad Católica del Ecuador, Quito 17012184, EcuadorSeveral microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm<sup>3</sup> HNO<sub>3</sub>, 1 cm<sup>3</sup> H<sub>2</sub>O<sub>2</sub>, and 1 cm<sup>3</sup> HClO<sub>4</sub>), an irradiation temperature of 210 °C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg<sup>−1</sup>) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (<i>Oreochromis niloticus</i>), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 µg·dm<sup>−3</sup> and 0.394 µg·dm<sup>−3</sup>, respectively.https://www.mdpi.com/2409-9279/3/2/45fish musclehigh-pressure vesselsmicrowave-assisted acid digestiontilapia (<i>Oreochromis niloticus</i>) |
| spellingShingle | Gabriela S. Yánez-Jácome David Romero-Estévez Hugo Navarrete Karina Simbaña-Farinango Pamela Y Vélez-Terreros Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry Methods and Protocols fish muscle high-pressure vessels microwave-assisted acid digestion tilapia (<i>Oreochromis niloticus</i>) |
| title | Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry |
| title_full | Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry |
| title_fullStr | Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry |
| title_full_unstemmed | Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry |
| title_short | Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry |
| title_sort | optimization of a digestion method to determine total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry |
| topic | fish muscle high-pressure vessels microwave-assisted acid digestion tilapia (<i>Oreochromis niloticus</i>) |
| url | https://www.mdpi.com/2409-9279/3/2/45 |
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