Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip
Extracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polyc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/9/347 |
| _version_ | 1797520070114541568 |
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| author | Gijung Kim Min Chul Park Seonae Jang Daeyoung Han Hojun Kim Wonjune Kim Honggu Chun Sunghoon Kim |
| author_facet | Gijung Kim Min Chul Park Seonae Jang Daeyoung Han Hojun Kim Wonjune Kim Honggu Chun Sunghoon Kim |
| author_sort | Gijung Kim |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polycarbonate membrane with 200 nm pores, positioned between two chambers, functions as the size-selective filter. Using the chip, EVs from cell culture media and human serum were separated. The separated EVs were analyzed by nanoparticle tracking analysis (NTA), scanning electron microscopy, and immunoblotting. The experimental results proved the selective separation of EVs in cell culture media and human serum. Moreover, the diffusion-based separation showed a high yield of EVs in human serum compared to ultracentrifuge-based separation. The EV recovery rate analyzed from NTA data was 42% for cell culture media samples. We expect the developed method to be a potential tool for EV separation for diagnosis and therapy because it does not require complicated processes such as immune, chemical reaction, and external force and is scalable by increasing the nanoporous membrane size. |
| first_indexed | 2024-03-10T07:51:37Z |
| format | Article |
| id | doaj.art-c344ce64f0244f28a173dded816c4ff7 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T07:51:37Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-c344ce64f0244f28a173dded816c4ff72023-11-22T12:13:01ZengMDPI AGBiosensors2079-63742021-09-0111934710.3390/bios11090347Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane ChipGijung Kim0Min Chul Park1Seonae Jang2Daeyoung Han3Hojun Kim4Wonjune Kim5Honggu Chun6Sunghoon Kim7Department of Biomedical Engineering, Korea University, Seoul 02841, KoreaMedicinal Bioconvergence Research Center, Suwon 16229, KoreaDepartment of Biomicrosystem Technology, Korea University, Seoul 02841, KoreaMedicinal Bioconvergence Research Center, Suwon 16229, KoreaDepartment of Biomedical Engineering, Korea University, Seoul 02841, KoreaDepartment of Biomedical Engineering, Korea University, Seoul 02841, KoreaDepartment of Biomedical Engineering, Korea University, Seoul 02841, KoreaMedicinal Bioconvergence Research Center, Suwon 16229, KoreaExtracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polycarbonate membrane with 200 nm pores, positioned between two chambers, functions as the size-selective filter. Using the chip, EVs from cell culture media and human serum were separated. The separated EVs were analyzed by nanoparticle tracking analysis (NTA), scanning electron microscopy, and immunoblotting. The experimental results proved the selective separation of EVs in cell culture media and human serum. Moreover, the diffusion-based separation showed a high yield of EVs in human serum compared to ultracentrifuge-based separation. The EV recovery rate analyzed from NTA data was 42% for cell culture media samples. We expect the developed method to be a potential tool for EV separation for diagnosis and therapy because it does not require complicated processes such as immune, chemical reaction, and external force and is scalable by increasing the nanoporous membrane size.https://www.mdpi.com/2079-6374/11/9/347exosomesdiffusion-based separationPC membranenanopore |
| spellingShingle | Gijung Kim Min Chul Park Seonae Jang Daeyoung Han Hojun Kim Wonjune Kim Honggu Chun Sunghoon Kim Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip Biosensors exosomes diffusion-based separation PC membrane nanopore |
| title | Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip |
| title_full | Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip |
| title_fullStr | Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip |
| title_full_unstemmed | Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip |
| title_short | Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip |
| title_sort | diffusion based separation of extracellular vesicles by nanoporous membrane chip |
| topic | exosomes diffusion-based separation PC membrane nanopore |
| url | https://www.mdpi.com/2079-6374/11/9/347 |
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