Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation
ATPPRT is a multi-protein allosteric enzyme where the regulatory protein HisZ enhances catalysis by HisGs. Here, the authors report catalytically impaired active site mutants of HisGs that are allosterically rescued by HisZ despite the HisZ:HisGs interface lying ~20 Å away from the active site.
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34960-9 |
| _version_ | 1811188233659219968 |
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| author | Gemma Fisher Marina Corbella Magnus S. Alphey John Nicholson Benjamin J. Read Shina C. L. Kamerlin Rafael G. da Silva |
| author_facet | Gemma Fisher Marina Corbella Magnus S. Alphey John Nicholson Benjamin J. Read Shina C. L. Kamerlin Rafael G. da Silva |
| author_sort | Gemma Fisher |
| collection | DOAJ |
| description | ATPPRT is a multi-protein allosteric enzyme where the regulatory protein HisZ enhances catalysis by HisGs. Here, the authors report catalytically impaired active site mutants of HisGs that are allosterically rescued by HisZ despite the HisZ:HisGs interface lying ~20 Å away from the active site. |
| first_indexed | 2024-04-11T14:16:08Z |
| format | Article |
| id | doaj.art-c345a6e16afe41afb597176f4ea0ea08 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T14:16:08Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c345a6e16afe41afb597176f4ea0ea082022-12-22T04:19:25ZengNature PortfolioNature Communications2041-17232022-12-0113111510.1038/s41467-022-34960-9Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisationGemma Fisher0Marina Corbella1Magnus S. Alphey2John Nicholson3Benjamin J. Read4Shina C. L. Kamerlin5Rafael G. da Silva6School of Biology, Biomedical Sciences Research Complex, University of St AndrewsScience for Life Laboratory, Department of Chemistry – BMC, Uppsala UniversitySchool of Biology, Biomedical Sciences Research Complex, University of St AndrewsSchool of Biology, Biomedical Sciences Research Complex, University of St AndrewsSchool of Biology, Biomedical Sciences Research Complex, University of St AndrewsScience for Life Laboratory, Department of Chemistry – BMC, Uppsala UniversitySchool of Biology, Biomedical Sciences Research Complex, University of St AndrewsATPPRT is a multi-protein allosteric enzyme where the regulatory protein HisZ enhances catalysis by HisGs. Here, the authors report catalytically impaired active site mutants of HisGs that are allosterically rescued by HisZ despite the HisZ:HisGs interface lying ~20 Å away from the active site.https://doi.org/10.1038/s41467-022-34960-9 |
| spellingShingle | Gemma Fisher Marina Corbella Magnus S. Alphey John Nicholson Benjamin J. Read Shina C. L. Kamerlin Rafael G. da Silva Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation Nature Communications |
| title | Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation |
| title_full | Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation |
| title_fullStr | Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation |
| title_full_unstemmed | Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation |
| title_short | Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation |
| title_sort | allosteric rescue of catalytically impaired atp phosphoribosyltransferase variants links protein dynamics to active site electrostatic preorganisation |
| url | https://doi.org/10.1038/s41467-022-34960-9 |
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