Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019
The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by pan...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/12/2404 |
| _version_ | 1797499994879557632 |
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| author | Sebastian Napp Francisco Llorente Cécile Beck Eduard Jose-Cunilleras Mercè Soler Lola Pailler-García Rayane Amaral Pilar Aguilera-Sepúlveda Maria Pifarré Rafael Molina-López Elena Obón Olga Nicolás Sylvie Lecollinet Miguel Ángel Jiménez-Clavero Núria Busquets |
| author_facet | Sebastian Napp Francisco Llorente Cécile Beck Eduard Jose-Cunilleras Mercè Soler Lola Pailler-García Rayane Amaral Pilar Aguilera-Sepúlveda Maria Pifarré Rafael Molina-López Elena Obón Olga Nicolás Sylvie Lecollinet Miguel Ángel Jiménez-Clavero Núria Busquets |
| author_sort | Sebastian Napp |
| collection | DOAJ |
| description | The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework. |
| first_indexed | 2024-03-10T03:55:28Z |
| format | Article |
| id | doaj.art-c346e1be1fcc41fc87c5c6298231c745 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T03:55:28Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c346e1be1fcc41fc87c5c6298231c7452023-11-23T10:57:27ZengMDPI AGViruses1999-49152021-11-011312240410.3390/v13122404Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019Sebastian Napp0Francisco Llorente1Cécile Beck2Eduard Jose-Cunilleras3Mercè Soler4Lola Pailler-García5Rayane Amaral6Pilar Aguilera-Sepúlveda7Maria Pifarré8Rafael Molina-López9Elena Obón10Olga Nicolás11Sylvie Lecollinet12Miguel Ángel Jiménez-Clavero13Núria Busquets14IRTA, Animal Health Research Centre (CReSA IRTA-UAB), 08193 Bellaterra, SpainCentro de Investigación en Sanidad Animal (CISA), Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), 28130 Valdeolmos, SpainUMR 1161 Virology, ANSES, INRAE, ENVA, ANSES Animal Health Laboratory, EURL for Equine Diseases, 94704 Maisons-Alfort, FranceDepartament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainServei de Prevenció en Salut Animal, Departament d’Acció Climàtica, Alimentació i Agenda Rural (DACC), 08007 Barcelona, SpainIRTA, Animal Health Research Centre (CReSA IRTA-UAB), 08193 Bellaterra, SpainUMR 1161 Virology, ANSES, INRAE, ENVA, ANSES Animal Health Laboratory, EURL for Equine Diseases, 94704 Maisons-Alfort, FranceCentro de Investigación en Sanidad Animal (CISA), Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), 28130 Valdeolmos, SpainCentre de Fauna dels Aiguamolls de l’Empordà, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 17486 Castelló d’Empúries, SpainCentre de Fauna de Torreferrussa, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 08130 Santa Perpètua de Mogoda, SpainCentre de Fauna de Torreferrussa, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 08130 Santa Perpètua de Mogoda, SpainCentre de Fauna de Vallcalent, Àrea de Gestió Ambiental Servei de Fauna i Flora, Forestal Catalana, 25199 Lleida, SpainUMR 1161 Virology, ANSES, INRAE, ENVA, ANSES Animal Health Laboratory, EURL for Equine Diseases, 94704 Maisons-Alfort, FranceCentro de Investigación en Sanidad Animal (CISA), Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), 28130 Valdeolmos, SpainIRTA, Animal Health Research Centre (CReSA IRTA-UAB), 08193 Bellaterra, SpainThe surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework.https://www.mdpi.com/1999-4915/13/12/2404West Nile virusUsutu virusBagaza virustick-borne encephalitis virusflavivirusesSpain |
| spellingShingle | Sebastian Napp Francisco Llorente Cécile Beck Eduard Jose-Cunilleras Mercè Soler Lola Pailler-García Rayane Amaral Pilar Aguilera-Sepúlveda Maria Pifarré Rafael Molina-López Elena Obón Olga Nicolás Sylvie Lecollinet Miguel Ángel Jiménez-Clavero Núria Busquets Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 Viruses West Nile virus Usutu virus Bagaza virus tick-borne encephalitis virus flaviviruses Spain |
| title | Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 |
| title_full | Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 |
| title_fullStr | Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 |
| title_full_unstemmed | Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 |
| title_short | Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 |
| title_sort | widespread circulation of flaviviruses in horses and birds in northeastern spain catalonia between 2010 and 2019 |
| topic | West Nile virus Usutu virus Bagaza virus tick-borne encephalitis virus flaviviruses Spain |
| url | https://www.mdpi.com/1999-4915/13/12/2404 |
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