Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts
Introduction: Inflammatory odontogenic cysts are lesions that develop from the cellular remains of odontogenesis. They are lesions that may recur after incomplete surgical enucleation with a variable clinical appearance depending on the clinical form. These clinical variations could be explained by...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Series: | Advances in Oral and Maxillofacial Surgery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667147623000432 |
| _version_ | 1827884634448330752 |
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| author | Mamadou Diatta Macoura Gadji Marie Jeo Diémé Souleymane Sarr Mamadou Keita Mouhammad Kane Abdou Ba Bintou Catherine Gassama Babacar Tamba Soukeye Dia Tine |
| author_facet | Mamadou Diatta Macoura Gadji Marie Jeo Diémé Souleymane Sarr Mamadou Keita Mouhammad Kane Abdou Ba Bintou Catherine Gassama Babacar Tamba Soukeye Dia Tine |
| author_sort | Mamadou Diatta |
| collection | DOAJ |
| description | Introduction: Inflammatory odontogenic cysts are lesions that develop from the cellular remains of odontogenesis. They are lesions that may recur after incomplete surgical enucleation with a variable clinical appearance depending on the clinical form. These clinical variations could be explained by the biological behaviour of the lesion cells which can be explored by the study of the cell proliferation index (Ki67). This justifies the initiation of this work whose objective was to study the level of expression of Ki67 and to measure the index of proliferation in odontogenic inflammatory cysts. Material and method: This was a cross-sectional study with an analytical aim on surgical parts of odontogenic inflammatory cysts over a period of 31 months in the odontostomatology department of the Hôpital Général Idrissa Pouye. The Ki67 immunohistochemistry study was performed on 3μ thick histological sections of paraffin-fixed tissue. The inclusion criteria were any surgical specimen of odontogenic inflammatory cyst that we could collect. The variables studied were sociodemographic, clinical and histopathological. Results: Radiculo-dental cysts represented 80.5% of the surgical specimens collected and residual cysts 12.2%. The percentage of Ki67-labelled cells was greater than 15% in 80.5% of the odontogenic inflammatory cysts and less than 7% in 19.5% of the cases. In addition, intense labelling was noted in radiculo-dental cysts and weak labelling in the other clinical forms of inflammatory odontogenic cysts obtained. An association was noted between the percentage of Ki67 labelled cells and histological types (p-value <0.001); but also between the intensity of Ki67 labelling and histological types (p-value <0.001). Conclusion: The cell proliferation index (Ki67) could be used as a prognostic biomarker in odontogenic inflammatory cysts. |
| first_indexed | 2024-03-12T19:32:53Z |
| format | Article |
| id | doaj.art-c34730e0c2454029b736084877145d71 |
| institution | Directory Open Access Journal |
| issn | 2667-1476 |
| language | English |
| last_indexed | 2024-03-12T19:32:53Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Oral and Maxillofacial Surgery |
| spelling | doaj.art-c34730e0c2454029b736084877145d712023-08-02T04:25:14ZengElsevierAdvances in Oral and Maxillofacial Surgery2667-14762023-09-0111100431Study of the cell proliferation index (Ki67) in inflammatory odontogenic cystsMamadou Diatta0Macoura Gadji1Marie Jeo Diémé2Souleymane Sarr3Mamadou Keita4Mouhammad Kane5Abdou Ba6Bintou Catherine Gassama7Babacar Tamba8Soukeye Dia Tine9Oral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar (UCAD), Senegal; Service d'Odontostomatologie, Hôpital Général Idrissa Pouye, Dakar, Senegal; Corresponding author. Oral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar, BP :5005 Fann, Dakar, Senegal.Molecular Biology Service, Department of Pharmacy, Cheikh Anta Diop University of Dakar (UCAD), SenegalPathological Anatomy Service, Department of Medicine, Cheikh Anta Diop University of Dakar (UCAD), SenegalPathological Anatomy Service, Department of Medicine, Cheikh Anta Diop University of Dakar (UCAD), SenegalPathological Anatomy Service, Department of Medicine, Cheikh Anta Diop University of Dakar (UCAD), SenegalOral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar (UCAD), SenegalOral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar (UCAD), SenegalOral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar (UCAD), SenegalOral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar (UCAD), Senegal; Service d'Odontostomatologie, Hôpital Général Idrissa Pouye, Dakar, SenegalOral Surgery Service, Department of Odontology, Cheikh Anta Diop University of Dakar (UCAD), Senegal; Service d'Odontostomatologie, Hôpital Général Idrissa Pouye, Dakar, SenegalIntroduction: Inflammatory odontogenic cysts are lesions that develop from the cellular remains of odontogenesis. They are lesions that may recur after incomplete surgical enucleation with a variable clinical appearance depending on the clinical form. These clinical variations could be explained by the biological behaviour of the lesion cells which can be explored by the study of the cell proliferation index (Ki67). This justifies the initiation of this work whose objective was to study the level of expression of Ki67 and to measure the index of proliferation in odontogenic inflammatory cysts. Material and method: This was a cross-sectional study with an analytical aim on surgical parts of odontogenic inflammatory cysts over a period of 31 months in the odontostomatology department of the Hôpital Général Idrissa Pouye. The Ki67 immunohistochemistry study was performed on 3μ thick histological sections of paraffin-fixed tissue. The inclusion criteria were any surgical specimen of odontogenic inflammatory cyst that we could collect. The variables studied were sociodemographic, clinical and histopathological. Results: Radiculo-dental cysts represented 80.5% of the surgical specimens collected and residual cysts 12.2%. The percentage of Ki67-labelled cells was greater than 15% in 80.5% of the odontogenic inflammatory cysts and less than 7% in 19.5% of the cases. In addition, intense labelling was noted in radiculo-dental cysts and weak labelling in the other clinical forms of inflammatory odontogenic cysts obtained. An association was noted between the percentage of Ki67 labelled cells and histological types (p-value <0.001); but also between the intensity of Ki67 labelling and histological types (p-value <0.001). Conclusion: The cell proliferation index (Ki67) could be used as a prognostic biomarker in odontogenic inflammatory cysts.http://www.sciencedirect.com/science/article/pii/S2667147623000432Odontogenic inflammatory cystsCell proliferation index (Ki67) |
| spellingShingle | Mamadou Diatta Macoura Gadji Marie Jeo Diémé Souleymane Sarr Mamadou Keita Mouhammad Kane Abdou Ba Bintou Catherine Gassama Babacar Tamba Soukeye Dia Tine Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts Advances in Oral and Maxillofacial Surgery Odontogenic inflammatory cysts Cell proliferation index (Ki67) |
| title | Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts |
| title_full | Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts |
| title_fullStr | Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts |
| title_full_unstemmed | Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts |
| title_short | Study of the cell proliferation index (Ki67) in inflammatory odontogenic cysts |
| title_sort | study of the cell proliferation index ki67 in inflammatory odontogenic cysts |
| topic | Odontogenic inflammatory cysts Cell proliferation index (Ki67) |
| url | http://www.sciencedirect.com/science/article/pii/S2667147623000432 |
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