Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells
Abstract This study aims to observe the expression of microRNA (miR)‐634 in different gastric cancer cell lines and tissues, and to study the effects of miR‐634 on the proliferation, migration, and invasion of the gastric cancer cells. The miR‐634 mimics and miR‐634 inhibitors were transfected by le...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-03-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.1204 |
| _version_ | 1797390105626804224 |
|---|---|
| author | Jiao Guo Chun‐Dong Zhang Jia‐Xiang An Yun‐Yun Xiao Shuai Shao Nuo‐Ming Zhou Dong‐Qiu Dai |
| author_facet | Jiao Guo Chun‐Dong Zhang Jia‐Xiang An Yun‐Yun Xiao Shuai Shao Nuo‐Ming Zhou Dong‐Qiu Dai |
| author_sort | Jiao Guo |
| collection | DOAJ |
| description | Abstract This study aims to observe the expression of microRNA (miR)‐634 in different gastric cancer cell lines and tissues, and to study the effects of miR‐634 on the proliferation, migration, and invasion of the gastric cancer cells. The miR‐634 mimics and miR‐634 inhibitors were transfected by lentivirus into human gastric cancer SGC‐7901 and MGC‐803 cells, and the miR‐634 cells without transfection were used as the control group (NC group). The expression of miR‐634 in the transfected cells was detected by qRT‐PCR. Cell viability was measured by the CCK8 assay. The migration and invasion ability of the cells were detected by scratch assays and Transwell® chamber assays, respectively, and the luciferase assay verified the binding of miR‐634 to the target gene JAG1. The expression level of miR‐634 in gastric cancer tissues and cell lines was significantly lower than that in normal adjacent tissues and control cells. The survival of cells was significantly decreased, and number of cells migrating and invading was decreased in the miR‐634 mimics group. However, in the miR‐634 inhibitor group, the opposite results were observed. Over‐expression of miR‐634 inhibited the proliferation, migration, and invasion of gastric cancer cell lines, and the miR‐634 target gene was JAG1. |
| first_indexed | 2024-03-08T23:06:47Z |
| format | Article |
| id | doaj.art-c347a52cd67241d2b04f8572403ab5b0 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-08T23:06:47Z |
| publishDate | 2018-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-c347a52cd67241d2b04f8572403ab5b02023-12-15T12:32:12ZengWileyCancer Medicine2045-76342018-03-017377678710.1002/cam4.1204Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cellsJiao Guo0Chun‐Dong Zhang1Jia‐Xiang An2Yun‐Yun Xiao3Shuai Shao4Nuo‐Ming Zhou5Dong‐Qiu Dai6Department of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University Shenyang 110032 ChinaDepartment of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University Shenyang 110032 ChinaDepartment of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University Shenyang 110032 ChinaDepartment of Obstetrics and Gynecology the Shengjing Affiliated Hospital of China Medical University Shenyang 110004 ChinaDepartment of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University Shenyang 110032 ChinaDepartment of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University Shenyang 110032 ChinaDepartment of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University Shenyang 110032 ChinaAbstract This study aims to observe the expression of microRNA (miR)‐634 in different gastric cancer cell lines and tissues, and to study the effects of miR‐634 on the proliferation, migration, and invasion of the gastric cancer cells. The miR‐634 mimics and miR‐634 inhibitors were transfected by lentivirus into human gastric cancer SGC‐7901 and MGC‐803 cells, and the miR‐634 cells without transfection were used as the control group (NC group). The expression of miR‐634 in the transfected cells was detected by qRT‐PCR. Cell viability was measured by the CCK8 assay. The migration and invasion ability of the cells were detected by scratch assays and Transwell® chamber assays, respectively, and the luciferase assay verified the binding of miR‐634 to the target gene JAG1. The expression level of miR‐634 in gastric cancer tissues and cell lines was significantly lower than that in normal adjacent tissues and control cells. The survival of cells was significantly decreased, and number of cells migrating and invading was decreased in the miR‐634 mimics group. However, in the miR‐634 inhibitor group, the opposite results were observed. Over‐expression of miR‐634 inhibited the proliferation, migration, and invasion of gastric cancer cell lines, and the miR‐634 target gene was JAG1.https://doi.org/10.1002/cam4.1204Gastric cancerJAG1MGC‐803miR‐634SGC‐7901 |
| spellingShingle | Jiao Guo Chun‐Dong Zhang Jia‐Xiang An Yun‐Yun Xiao Shuai Shao Nuo‐Ming Zhou Dong‐Qiu Dai Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells Cancer Medicine Gastric cancer JAG1 MGC‐803 miR‐634 SGC‐7901 |
| title | Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells |
| title_full | Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells |
| title_fullStr | Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells |
| title_full_unstemmed | Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells |
| title_short | Expression of miR‐634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells |
| title_sort | expression of mir 634 in gastric carcinoma and its effects on proliferation migration and invasion of gastric cancer cells |
| topic | Gastric cancer JAG1 MGC‐803 miR‐634 SGC‐7901 |
| url | https://doi.org/10.1002/cam4.1204 |
| work_keys_str_mv | AT jiaoguo expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells AT chundongzhang expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells AT jiaxiangan expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells AT yunyunxiao expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells AT shuaishao expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells AT nuomingzhou expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells AT dongqiudai expressionofmir634ingastriccarcinomaanditseffectsonproliferationmigrationandinvasionofgastriccancercells |