A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean
Soybean aphid (<i>Aphis glycines</i> Matsumura) is one of the major limiting factors in soybean production. The mechanism of aphid resistance in soybean remains enigmatic as little information is available about the different mechanisms of antibiosis and antixenosis. Here, we used genome...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/15/5191 |
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| author | Luming Yao Biyun Yang Xiaohong Ma Shuangshuang Wang Zhe Guan Biao Wang Yina Jiang |
| author_facet | Luming Yao Biyun Yang Xiaohong Ma Shuangshuang Wang Zhe Guan Biao Wang Yina Jiang |
| author_sort | Luming Yao |
| collection | DOAJ |
| description | Soybean aphid (<i>Aphis glycines</i> Matsumura) is one of the major limiting factors in soybean production. The mechanism of aphid resistance in soybean remains enigmatic as little information is available about the different mechanisms of antibiosis and antixenosis. Here, we used genome-wide gene expression profiling of aphid susceptible, antibiotic, and antixenotic genotypes to investigate the underlying aphid–plant interaction mechanisms. The high expression correlation between infested and non-infested genotypes indicated that the response to aphid was controlled by a small subset of genes. Plant response to aphid infestation was faster in antibiotic genotype and the interaction in antixenotic genotype was moderation. The expression patterns of transcription factor genes in susceptible and antixenotic genotypes clustered together and were distant from those of antibiotic genotypes. Among them APETALA 2/ethylene response factors (AP2/ERF), v-myb avian myeloblastosis viral oncogene homolog (MYB), and the transcription factor contained conserved WRKYGQK domain (WRKY) were proposed to play dominant roles. The jasmonic acid-responsive pathway was dominant in aphid–soybean interaction, and salicylic acid pathway played an important role in antibiotic genotype. Callose deposition was more rapid and efficient in antibiotic genotype, while reactive oxygen species were not involved in the response to aphid attack in resistant genotypes. Our study helps to uncover important genes associated with aphid-attack response in soybean genotypes expressing antibiosis and antixenosis. |
| first_indexed | 2024-03-10T18:17:15Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:17:15Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c34a17714d384f18a2c3a83511df60902023-11-20T07:36:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115519110.3390/ijms21155191A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in SoybeanLuming Yao0Biyun Yang1Xiaohong Ma2Shuangshuang Wang3Zhe Guan4Biao Wang5Yina Jiang6School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200241, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200241, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200241, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Life Sciences, East China Normal University, Shanghai 200241, ChinaSoybean aphid (<i>Aphis glycines</i> Matsumura) is one of the major limiting factors in soybean production. The mechanism of aphid resistance in soybean remains enigmatic as little information is available about the different mechanisms of antibiosis and antixenosis. Here, we used genome-wide gene expression profiling of aphid susceptible, antibiotic, and antixenotic genotypes to investigate the underlying aphid–plant interaction mechanisms. The high expression correlation between infested and non-infested genotypes indicated that the response to aphid was controlled by a small subset of genes. Plant response to aphid infestation was faster in antibiotic genotype and the interaction in antixenotic genotype was moderation. The expression patterns of transcription factor genes in susceptible and antixenotic genotypes clustered together and were distant from those of antibiotic genotypes. Among them APETALA 2/ethylene response factors (AP2/ERF), v-myb avian myeloblastosis viral oncogene homolog (MYB), and the transcription factor contained conserved WRKYGQK domain (WRKY) were proposed to play dominant roles. The jasmonic acid-responsive pathway was dominant in aphid–soybean interaction, and salicylic acid pathway played an important role in antibiotic genotype. Callose deposition was more rapid and efficient in antibiotic genotype, while reactive oxygen species were not involved in the response to aphid attack in resistant genotypes. Our study helps to uncover important genes associated with aphid-attack response in soybean genotypes expressing antibiosis and antixenosis.https://www.mdpi.com/1422-0067/21/15/5191<i>Glycine max</i><i>Aphis glycine</i>plant–insect interactionantibiosisantixenosis |
| spellingShingle | Luming Yao Biyun Yang Xiaohong Ma Shuangshuang Wang Zhe Guan Biao Wang Yina Jiang A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean International Journal of Molecular Sciences <i>Glycine max</i> <i>Aphis glycine</i> plant–insect interaction antibiosis antixenosis |
| title | A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean |
| title_full | A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean |
| title_fullStr | A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean |
| title_full_unstemmed | A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean |
| title_short | A Genome-Wide View of Transcriptional Responses during <i>Aphis glycines</i> Infestation in Soybean |
| title_sort | genome wide view of transcriptional responses during i aphis glycines i infestation in soybean |
| topic | <i>Glycine max</i> <i>Aphis glycine</i> plant–insect interaction antibiosis antixenosis |
| url | https://www.mdpi.com/1422-0067/21/15/5191 |
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