Anti-infective characteristics of a new Carbothane ventricular assist device driveline
Objectives: Driveline infections are a major complication of ventricular assist device (VAD) therapy. A newly introduced Carbothane driveline has preliminarily demonstrated anti-infective potential against driveline infections. This study aimed to comprehensively assess the anti-biofilm capability o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207523000217 |
| _version_ | 1797800998180225024 |
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| author | Yue Qu David McGiffin Lina Duque Sanchez Thomas Gengenbach Chris Easton Helmut Thissen Anton Y. Peleg |
| author_facet | Yue Qu David McGiffin Lina Duque Sanchez Thomas Gengenbach Chris Easton Helmut Thissen Anton Y. Peleg |
| author_sort | Yue Qu |
| collection | DOAJ |
| description | Objectives: Driveline infections are a major complication of ventricular assist device (VAD) therapy. A newly introduced Carbothane driveline has preliminarily demonstrated anti-infective potential against driveline infections. This study aimed to comprehensively assess the anti-biofilm capability of the Carbothane driveline and explore its physicochemical characteristics. Methods: We assessed the Carbothane driveline against biofilm formation of leading microorganisms causing VAD driveline infections, including Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans, using novel in vitro biofilm assays mimicking different infection micro-environments. The importance of physicochemical properties of the Carbothane driveline in microorganism-device interactions were analyzed, particularly focusing on the surface chemistry. The role of micro-gaps in driveline tunnels on biofilm migration was also examined. Results: All organisms were able to attach to the smooth and velour sections of the Carbothane driveline. Early microbial adherence, at least for S. aureus and S. epidermidis, did not proceed to the formation of mature biofilms in a drip-flow biofilm reactor mimicking the driveline exit site environment. The presence of a driveline tunnel however, promoted staphylococcal biofilm formation on the Carbothane driveline. Physicochemical analysis of the Carbothane driveline revealed surface characteristics that may have contributed to its anti-biofilm activity, such as the aliphatic nature of its surface. The presence of micro-gaps in the tunnel facilitated biofilm migration of the studied bacterial species. Conclusion: This study provides experimental evidence to support the anti-biofilm activity of the Carbothane driveline and uncovered specific physicochemical features that may explain its ability to inhibit biofilm formation. |
| first_indexed | 2024-03-13T04:43:44Z |
| format | Article |
| id | doaj.art-c34a8df5d38a4a1590bdcc5902087403 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-03-13T04:43:44Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-c34a8df5d38a4a1590bdcc59020874032023-06-19T04:29:34ZengElsevierBiofilm2590-20752023-12-015100124Anti-infective characteristics of a new Carbothane ventricular assist device drivelineYue Qu0David McGiffin1Lina Duque Sanchez2Thomas Gengenbach3Chris Easton4Helmut Thissen5Anton Y. Peleg6Infection Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Clayton, Victoria, 3800, Australia; Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria, 3004, AustraliaDepartment of Cardiothoracic Surgery, The Alfred and Monash University, Melbourne, Victoria, 3004, AustraliaCommonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing, Clayton, Victoria, 3168, Australia; Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, Parkville, Victoria, 3052, AustraliaCommonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing, Clayton, Victoria, 3168, AustraliaCommonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing, Clayton, Victoria, 3168, AustraliaCommonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing, Clayton, Victoria, 3168, AustraliaInfection Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Clayton, Victoria, 3800, Australia; Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria, 3004, Australia; Corresponding author. Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Australia.Objectives: Driveline infections are a major complication of ventricular assist device (VAD) therapy. A newly introduced Carbothane driveline has preliminarily demonstrated anti-infective potential against driveline infections. This study aimed to comprehensively assess the anti-biofilm capability of the Carbothane driveline and explore its physicochemical characteristics. Methods: We assessed the Carbothane driveline against biofilm formation of leading microorganisms causing VAD driveline infections, including Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans, using novel in vitro biofilm assays mimicking different infection micro-environments. The importance of physicochemical properties of the Carbothane driveline in microorganism-device interactions were analyzed, particularly focusing on the surface chemistry. The role of micro-gaps in driveline tunnels on biofilm migration was also examined. Results: All organisms were able to attach to the smooth and velour sections of the Carbothane driveline. Early microbial adherence, at least for S. aureus and S. epidermidis, did not proceed to the formation of mature biofilms in a drip-flow biofilm reactor mimicking the driveline exit site environment. The presence of a driveline tunnel however, promoted staphylococcal biofilm formation on the Carbothane driveline. Physicochemical analysis of the Carbothane driveline revealed surface characteristics that may have contributed to its anti-biofilm activity, such as the aliphatic nature of its surface. The presence of micro-gaps in the tunnel facilitated biofilm migration of the studied bacterial species. Conclusion: This study provides experimental evidence to support the anti-biofilm activity of the Carbothane driveline and uncovered specific physicochemical features that may explain its ability to inhibit biofilm formation.http://www.sciencedirect.com/science/article/pii/S2590207523000217Ventricular assist device drivelineAnti-infectiveCarbothaneBiofilmsSurface chemistry |
| spellingShingle | Yue Qu David McGiffin Lina Duque Sanchez Thomas Gengenbach Chris Easton Helmut Thissen Anton Y. Peleg Anti-infective characteristics of a new Carbothane ventricular assist device driveline Biofilm Ventricular assist device driveline Anti-infective Carbothane Biofilms Surface chemistry |
| title | Anti-infective characteristics of a new Carbothane ventricular assist device driveline |
| title_full | Anti-infective characteristics of a new Carbothane ventricular assist device driveline |
| title_fullStr | Anti-infective characteristics of a new Carbothane ventricular assist device driveline |
| title_full_unstemmed | Anti-infective characteristics of a new Carbothane ventricular assist device driveline |
| title_short | Anti-infective characteristics of a new Carbothane ventricular assist device driveline |
| title_sort | anti infective characteristics of a new carbothane ventricular assist device driveline |
| topic | Ventricular assist device driveline Anti-infective Carbothane Biofilms Surface chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2590207523000217 |
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