Effects of bisphenol A on murine salivary glands and human tumor cell lines
Bisphenol A (BPA) is an endocrine-disrupting chemical with a potential role in endocrine cancers. However, the effects of BPA on the salivary glands have been barely explored. We investigated the impact of in vivo sub-chronic exposure to BPA and its in vitro effects on human salivary gland mucoepide...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Experimental and Molecular Pathology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0014480023000217 |
| _version_ | 1827583928769183744 |
|---|---|
| author | Gabriela Kelly da Silva José Alcides Almeida de Arruda Tatiana Fernandes Araújo Almeida Sicília Rezende Oliveira Paula Alves da Silva Rocha Ricardo Alves Mesquita Zenilda de Lourdes Cardeal Helvécio Costa Menezes Ivana Márcia Alves Diniz Soraia Macari Andréia Machado Leopoldino Tarcília Aparecida Silva |
| author_facet | Gabriela Kelly da Silva José Alcides Almeida de Arruda Tatiana Fernandes Araújo Almeida Sicília Rezende Oliveira Paula Alves da Silva Rocha Ricardo Alves Mesquita Zenilda de Lourdes Cardeal Helvécio Costa Menezes Ivana Márcia Alves Diniz Soraia Macari Andréia Machado Leopoldino Tarcília Aparecida Silva |
| author_sort | Gabriela Kelly da Silva |
| collection | DOAJ |
| description | Bisphenol A (BPA) is an endocrine-disrupting chemical with a potential role in endocrine cancers. However, the effects of BPA on the salivary glands have been barely explored. We investigated the impact of in vivo sub-chronic exposure to BPA and its in vitro effects on human salivary gland mucoepidermoid carcinoma cell lines. Male and female mice were exposed to BPA (30 mg/kg/day). Sublingual and submandibular salivary glands from an estrogen-deficiency model were also analyzed. BPA concentration in salivary glands was evaluated by gas chromatography coupled to ion trap mass spectrometry. Immunohistochemical analysis using anti-p63 and anti-α-SMA antibodies was performed on mouse salivary gland tissues. Gene expression of estrogen receptors alpha and beta, P63 and α-SMA was quantified in mouse salivary gland and/or mucoepidermoid (UM-HMC-1 and UM-HMC-3A) cell lines. Cell viability, p63 and Ki-67 immunostaining were evaluated in vitro. BPA disrupted the tissue architecture of the submandibular and sublingual glands, particularly in female mice, and increased the expression of estrogen receptors and p63, effects that were accompanied by significant BPA accumulation in these tissues. Conversely, ovariectomy slightly impacted BPA-induced morphological changes. In vitro, BPA did not affect the proliferation of neoplastic cells, but augmented the expression of p63 and estrogen receptors. The present data highlight a potential harmful effect of BPA on salivary gland tissues, particularly in female mice, and salivary gland tumor cells. Our findings suggest that estrogen-dependent pathways may orchestrate the effects of BPA in salivary glands. |
| first_indexed | 2024-03-08T23:14:44Z |
| format | Article |
| id | doaj.art-c34cf0f477e4461c99b9dd2efaa01b44 |
| institution | Directory Open Access Journal |
| issn | 1096-0945 |
| language | English |
| last_indexed | 2024-03-08T23:14:44Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Experimental and Molecular Pathology |
| spelling | doaj.art-c34cf0f477e4461c99b9dd2efaa01b442023-12-15T07:21:47ZengElsevierExperimental and Molecular Pathology1096-09452023-12-01134104870Effects of bisphenol A on murine salivary glands and human tumor cell linesGabriela Kelly da Silva0José Alcides Almeida de Arruda1Tatiana Fernandes Araújo Almeida2Sicília Rezende Oliveira3Paula Alves da Silva Rocha4Ricardo Alves Mesquita5Zenilda de Lourdes Cardeal6Helvécio Costa Menezes7Ivana Márcia Alves Diniz8Soraia Macari9Andréia Machado Leopoldino10Tarcília Aparecida Silva11Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, BrazilDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Corresponding author at: Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, room 3204, Belo Horizonte, Minas Gerais CEP: 31.270-910, Brazil.Bisphenol A (BPA) is an endocrine-disrupting chemical with a potential role in endocrine cancers. However, the effects of BPA on the salivary glands have been barely explored. We investigated the impact of in vivo sub-chronic exposure to BPA and its in vitro effects on human salivary gland mucoepidermoid carcinoma cell lines. Male and female mice were exposed to BPA (30 mg/kg/day). Sublingual and submandibular salivary glands from an estrogen-deficiency model were also analyzed. BPA concentration in salivary glands was evaluated by gas chromatography coupled to ion trap mass spectrometry. Immunohistochemical analysis using anti-p63 and anti-α-SMA antibodies was performed on mouse salivary gland tissues. Gene expression of estrogen receptors alpha and beta, P63 and α-SMA was quantified in mouse salivary gland and/or mucoepidermoid (UM-HMC-1 and UM-HMC-3A) cell lines. Cell viability, p63 and Ki-67 immunostaining were evaluated in vitro. BPA disrupted the tissue architecture of the submandibular and sublingual glands, particularly in female mice, and increased the expression of estrogen receptors and p63, effects that were accompanied by significant BPA accumulation in these tissues. Conversely, ovariectomy slightly impacted BPA-induced morphological changes. In vitro, BPA did not affect the proliferation of neoplastic cells, but augmented the expression of p63 and estrogen receptors. The present data highlight a potential harmful effect of BPA on salivary gland tissues, particularly in female mice, and salivary gland tumor cells. Our findings suggest that estrogen-dependent pathways may orchestrate the effects of BPA in salivary glands.http://www.sciencedirect.com/science/article/pii/S0014480023000217Bisphenol AEndocrine-disrupting chemicalEnvironmental pollutantEstrogen receptorsSalivary glands |
| spellingShingle | Gabriela Kelly da Silva José Alcides Almeida de Arruda Tatiana Fernandes Araújo Almeida Sicília Rezende Oliveira Paula Alves da Silva Rocha Ricardo Alves Mesquita Zenilda de Lourdes Cardeal Helvécio Costa Menezes Ivana Márcia Alves Diniz Soraia Macari Andréia Machado Leopoldino Tarcília Aparecida Silva Effects of bisphenol A on murine salivary glands and human tumor cell lines Experimental and Molecular Pathology Bisphenol A Endocrine-disrupting chemical Environmental pollutant Estrogen receptors Salivary glands |
| title | Effects of bisphenol A on murine salivary glands and human tumor cell lines |
| title_full | Effects of bisphenol A on murine salivary glands and human tumor cell lines |
| title_fullStr | Effects of bisphenol A on murine salivary glands and human tumor cell lines |
| title_full_unstemmed | Effects of bisphenol A on murine salivary glands and human tumor cell lines |
| title_short | Effects of bisphenol A on murine salivary glands and human tumor cell lines |
| title_sort | effects of bisphenol a on murine salivary glands and human tumor cell lines |
| topic | Bisphenol A Endocrine-disrupting chemical Environmental pollutant Estrogen receptors Salivary glands |
| url | http://www.sciencedirect.com/science/article/pii/S0014480023000217 |
| work_keys_str_mv | AT gabrielakellydasilva effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT josealcidesalmeidadearruda effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT tatianafernandesaraujoalmeida effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT siciliarezendeoliveira effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT paulaalvesdasilvarocha effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT ricardoalvesmesquita effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT zenildadelourdescardeal effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT helveciocostamenezes effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT ivanamarciaalvesdiniz effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT soraiamacari effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT andreiamachadoleopoldino effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines AT tarciliaaparecidasilva effectsofbisphenolaonmurinesalivaryglandsandhumantumorcelllines |