A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris
Candida auris is an emerging multidrug-resistant fungal pathogen that can cause severe and deadly infections. To date, C. auris has spurred outbreaks in healthcare settings in thirty-three countries across five continents. To control and potentially prevent its spread, there is an urgent need for po...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.826694/full |
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| author | Pei-Wei Lee Marissa Totten Liben Chen Fan-En Chen Alexander Y. Trick Kushagra Shah Hoan Thanh Ngo Mei Jin Kuangwen Hsieh Sean X. Zhang Tza-Huei Wang Tza-Huei Wang Tza-Huei Wang |
| author_facet | Pei-Wei Lee Marissa Totten Liben Chen Fan-En Chen Alexander Y. Trick Kushagra Shah Hoan Thanh Ngo Mei Jin Kuangwen Hsieh Sean X. Zhang Tza-Huei Wang Tza-Huei Wang Tza-Huei Wang |
| author_sort | Pei-Wei Lee |
| collection | DOAJ |
| description | Candida auris is an emerging multidrug-resistant fungal pathogen that can cause severe and deadly infections. To date, C. auris has spurred outbreaks in healthcare settings in thirty-three countries across five continents. To control and potentially prevent its spread, there is an urgent need for point-of-care (POC) diagnostics that can rapidly screen patients, close patient contacts, and surveil environmental sources. Droplet magnetofluidics (DM), which leverages nucleic acid-binding magnetic beads for realizing POC-amenable nucleic acid detection platforms, offers a promising solution. Herein, we report the first DM device—coined POC.auris—for POC detection of C. auris. As part of POC.auris, we have incorporated a handheld cell lysis module that lyses C. auris cells with 2 min hands-on time. Subsequently, within the palm-sized and automated DM device, C. auris and control DNA are magnetically extracted and purified by a motorized magnetic arm and finally amplified via a duplex real-time quantitative PCR assay by a miniaturized rapid PCR module and a miniaturized fluorescence detector—all in ≤30 min. For demonstration, we use POC.auris to detect C. auris isolates from 3 major clades, with no cross reactivity against other Candida species and a limit of detection of ∼300 colony forming units per mL. Taken together, POC.auris presents a potentially useful tool for combating C. auris. |
| first_indexed | 2024-12-22T16:38:08Z |
| format | Article |
| id | doaj.art-c34d63b1ffe0428b911cff30a510fef1 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T16:38:08Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-c34d63b1ffe0428b911cff30a510fef12022-12-21T18:19:55ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.826694826694A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida aurisPei-Wei Lee0Marissa Totten1Liben Chen2Fan-En Chen3Alexander Y. Trick4Kushagra Shah5Hoan Thanh Ngo6Mei Jin7Kuangwen Hsieh8Sean X. Zhang9Tza-Huei Wang10Tza-Huei Wang11Tza-Huei Wang12Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDivision of Microbiology, Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, United StatesDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDivision of Microbiology, Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, United StatesDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United StatesDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United StatesInstitute of NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United StatesCandida auris is an emerging multidrug-resistant fungal pathogen that can cause severe and deadly infections. To date, C. auris has spurred outbreaks in healthcare settings in thirty-three countries across five continents. To control and potentially prevent its spread, there is an urgent need for point-of-care (POC) diagnostics that can rapidly screen patients, close patient contacts, and surveil environmental sources. Droplet magnetofluidics (DM), which leverages nucleic acid-binding magnetic beads for realizing POC-amenable nucleic acid detection platforms, offers a promising solution. Herein, we report the first DM device—coined POC.auris—for POC detection of C. auris. As part of POC.auris, we have incorporated a handheld cell lysis module that lyses C. auris cells with 2 min hands-on time. Subsequently, within the palm-sized and automated DM device, C. auris and control DNA are magnetically extracted and purified by a motorized magnetic arm and finally amplified via a duplex real-time quantitative PCR assay by a miniaturized rapid PCR module and a miniaturized fluorescence detector—all in ≤30 min. For demonstration, we use POC.auris to detect C. auris isolates from 3 major clades, with no cross reactivity against other Candida species and a limit of detection of ∼300 colony forming units per mL. Taken together, POC.auris presents a potentially useful tool for combating C. auris.https://www.frontiersin.org/articles/10.3389/fbioe.2022.826694/fulldroplet magnetofluidicspoint-of-care diagnosticssample preparationlysisminiaturized rapid PCRCandida auris |
| spellingShingle | Pei-Wei Lee Marissa Totten Liben Chen Fan-En Chen Alexander Y. Trick Kushagra Shah Hoan Thanh Ngo Mei Jin Kuangwen Hsieh Sean X. Zhang Tza-Huei Wang Tza-Huei Wang Tza-Huei Wang A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris Frontiers in Bioengineering and Biotechnology droplet magnetofluidics point-of-care diagnostics sample preparation lysis miniaturized rapid PCR Candida auris |
| title | A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris |
| title_full | A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris |
| title_fullStr | A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris |
| title_full_unstemmed | A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris |
| title_short | A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris |
| title_sort | portable droplet magnetofluidic device for point of care detection of multidrug resistant candida auris |
| topic | droplet magnetofluidics point-of-care diagnostics sample preparation lysis miniaturized rapid PCR Candida auris |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.826694/full |
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