Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes
Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 pe...
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| Format: | Article |
| Language: | English |
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National Electronic Information Consortium (NEICON)
2021-12-01
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| Series: | Спортивная медицина: наука и практика |
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| Online Access: | https://www.smjournal.ru/jour/article/view/298 |
| _version_ | 1797372538061324288 |
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| author | A. V. Elikov |
| author_facet | A. V. Elikov |
| author_sort | A. V. Elikov |
| collection | DOAJ |
| description | Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent. |
| first_indexed | 2024-03-08T18:36:07Z |
| format | Article |
| id | doaj.art-c34f370362f94dd691f01a8db8313083 |
| institution | Directory Open Access Journal |
| issn | 2223-2524 2587-9014 |
| language | English |
| last_indexed | 2024-03-08T18:36:07Z |
| publishDate | 2021-12-01 |
| publisher | National Electronic Information Consortium (NEICON) |
| record_format | Article |
| series | Спортивная медицина: наука и практика |
| spelling | doaj.art-c34f370362f94dd691f01a8db83130832023-12-29T11:27:48ZengNational Electronic Information Consortium (NEICON)Спортивная медицина: наука и практика2223-25242587-90142021-12-01113121710.47529/2223-2524.2021.3.5255Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletesA. V. Elikov0Kirov State Medical UniversityObjective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.https://www.smjournal.ru/jour/article/view/298detrainingathleteserythrocytesfree radical oxidationantioxidant protection |
| spellingShingle | A. V. Elikov Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes Спортивная медицина: наука и практика detraining athletes erythrocytes free radical oxidation antioxidant protection |
| title | Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes |
| title_full | Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes |
| title_fullStr | Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes |
| title_full_unstemmed | Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes |
| title_short | Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes |
| title_sort | some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes |
| topic | detraining athletes erythrocytes free radical oxidation antioxidant protection |
| url | https://www.smjournal.ru/jour/article/view/298 |
| work_keys_str_mv | AT avelikov someindicatorsoffreeradicaloxidationandantioxidantprotectionintheerythrocytesofformerathletes |