A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves
Abstract Background Bamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems in bamboo are significantly limited by the dependence on bamboo regene...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13007-023-00993-4 |
| _version_ | 1797864586903289856 |
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| author | Huayu Sun Sining Wang Chenglei Zhu Kebin Yang Yan Liu Zhimin Gao |
| author_facet | Huayu Sun Sining Wang Chenglei Zhu Kebin Yang Yan Liu Zhimin Gao |
| author_sort | Huayu Sun |
| collection | DOAJ |
| description | Abstract Background Bamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems in bamboo are significantly limited by the dependence on bamboo regeneration capability. The way to improve the flavonoid content in bamboo leaves through biotechnology is still not feasible. Results Here, we developed an in-planta, Agrobacterium-mediated gene expression method for exogenous genes via wounding and vacuum in bamboo. We demonstrated that the RUBY served as a reporter efficiently expressed in bamboo leaves and shoots, albeit unable to integrate into the chromosome. We have also developed a gene editing system by creating an in situ mutant of the bamboo violaxanthin de-epoxidase (PeVDE) gene in bamboo leaves, with lower NPQ values under the fluorometer, which can serve as a native reporter for gene editing. Furthermore, the bamboo leaves with increased flavonoid content were achieved by knocking out the cinnamoyl-CoA reductase genes. Conclusions Our method can be applied for the functional characterization of novel genes in a short time and is helpful for bamboo leaf flavonoid biotechnology breeding in the future. |
| first_indexed | 2024-04-09T22:55:25Z |
| format | Article |
| id | doaj.art-c3533cf25af94f6f81ca112fff0b7a8a |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-04-09T22:55:25Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-c3533cf25af94f6f81ca112fff0b7a8a2023-03-22T11:20:04ZengBMCPlant Methods1746-48112023-03-0119111010.1186/s13007-023-00993-4A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leavesHuayu Sun0Sining Wang1Chenglei Zhu2Kebin Yang3Yan Liu4Zhimin Gao5Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and TechnologyKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and TechnologyKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and TechnologyKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and TechnologyKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and TechnologyKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and TechnologyAbstract Background Bamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems in bamboo are significantly limited by the dependence on bamboo regeneration capability. The way to improve the flavonoid content in bamboo leaves through biotechnology is still not feasible. Results Here, we developed an in-planta, Agrobacterium-mediated gene expression method for exogenous genes via wounding and vacuum in bamboo. We demonstrated that the RUBY served as a reporter efficiently expressed in bamboo leaves and shoots, albeit unable to integrate into the chromosome. We have also developed a gene editing system by creating an in situ mutant of the bamboo violaxanthin de-epoxidase (PeVDE) gene in bamboo leaves, with lower NPQ values under the fluorometer, which can serve as a native reporter for gene editing. Furthermore, the bamboo leaves with increased flavonoid content were achieved by knocking out the cinnamoyl-CoA reductase genes. Conclusions Our method can be applied for the functional characterization of novel genes in a short time and is helpful for bamboo leaf flavonoid biotechnology breeding in the future.https://doi.org/10.1186/s13007-023-00993-4BambooIn-planta transformationGene editingViolaxanthin de-epoxidaseBamboo leaf flavonoid |
| spellingShingle | Huayu Sun Sining Wang Chenglei Zhu Kebin Yang Yan Liu Zhimin Gao A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves Plant Methods Bamboo In-planta transformation Gene editing Violaxanthin de-epoxidase Bamboo leaf flavonoid |
| title | A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves |
| title_full | A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves |
| title_fullStr | A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves |
| title_full_unstemmed | A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves |
| title_short | A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves |
| title_sort | new biotechnology for in planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves |
| topic | Bamboo In-planta transformation Gene editing Violaxanthin de-epoxidase Bamboo leaf flavonoid |
| url | https://doi.org/10.1186/s13007-023-00993-4 |
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