Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study
Background Rise in the number of healthcare associated or hospital acquired infections is a major problem affecting the global healthcare sector. We evaluated superior antibacterial and antibiofilm photodynamic therapy (aPDT) using malachite green encapsulated mesoporous silica nanoparticles (MG-MSN...
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PeerJ Inc.
2019-09-01
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| author | Parasuraman Paramanantham Busi Siddhardha Sruthil Lal SB Alok Sharan Abdullah A. Alyousef Mohammed Saeed Al Dosary Mohammed Arshad Asad Syed |
| author_facet | Parasuraman Paramanantham Busi Siddhardha Sruthil Lal SB Alok Sharan Abdullah A. Alyousef Mohammed Saeed Al Dosary Mohammed Arshad Asad Syed |
| author_sort | Parasuraman Paramanantham |
| collection | DOAJ |
| description | Background Rise in the number of healthcare associated or hospital acquired infections is a major problem affecting the global healthcare sector. We evaluated superior antibacterial and antibiofilm photodynamic therapy (aPDT) using malachite green encapsulated mesoporous silica nanoparticles (MG-MSN) against Staphylococcus aureus and Escherichia coli, which are known to be major causative agents of nosocomial infections. Methods Malachite green (MG) was encapsulated on mesoporous silica nanoparticles (MSN). Fourier-transform infrared spectroscopy, Transmission electron microscopy, and spectroscopic analysis were performed to characterize the MG-MSN. The antimicrobial efficacies of MSN, MG, and MG-MSN were investigated and the results were recorded. Results MG-MSN was effective against both the tested bacteria. S. aureus was more phototoxic to MG-MSN compared to E. coli. The antibiofilm efficacy of MG-MSN on E. coli and S. aureus was also studied. Biofilm inhibition was 65.68 ± 2.62% in E. coli and 79.66 ± 3.82% in S. aureus. Cell viability assay, exopolysaccharides quantification, and confocal laser scanning microscopy studies also revealed the enhanced antibiofilm activity of MG-MSN when used as a potential photosensitizer for aPDT. This study can be extended to eradicate these strains from localized superficial infections and medical appliances, preventing nosocomial infections. |
| first_indexed | 2024-03-09T06:37:35Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:37:35Z |
| publishDate | 2019-09-01 |
| publisher | PeerJ Inc. |
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| series | PeerJ |
| spelling | doaj.art-c35690b331584cacaff3bf677ee800ec2023-12-03T10:55:49ZengPeerJ Inc.PeerJ2167-83592019-09-017e745410.7717/peerj.7454Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro studyParasuraman Paramanantham0Busi Siddhardha1Sruthil Lal SB2Alok Sharan3Abdullah A. Alyousef4Mohammed Saeed Al Dosary5Mohammed Arshad6Asad Syed7Department of Microbiology, School of Life Sciences, Pondicherry University, Pondicherry, IndiaDepartment of Microbiology, School of Life Sciences, Pondicherry University, Pondicherry, IndiaDepartment of Physics, School of Physical, Chemical and Applied Sciences, Pondicherry University, Pondicherry, IndiaDepartment of Physics, School of Physical, Chemical and Applied Sciences, Pondicherry University, Pondicherry, IndiaMicrobiology Research Group, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaMicrobiology Research Group, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaMicrobiology Research Group, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaBackground Rise in the number of healthcare associated or hospital acquired infections is a major problem affecting the global healthcare sector. We evaluated superior antibacterial and antibiofilm photodynamic therapy (aPDT) using malachite green encapsulated mesoporous silica nanoparticles (MG-MSN) against Staphylococcus aureus and Escherichia coli, which are known to be major causative agents of nosocomial infections. Methods Malachite green (MG) was encapsulated on mesoporous silica nanoparticles (MSN). Fourier-transform infrared spectroscopy, Transmission electron microscopy, and spectroscopic analysis were performed to characterize the MG-MSN. The antimicrobial efficacies of MSN, MG, and MG-MSN were investigated and the results were recorded. Results MG-MSN was effective against both the tested bacteria. S. aureus was more phototoxic to MG-MSN compared to E. coli. The antibiofilm efficacy of MG-MSN on E. coli and S. aureus was also studied. Biofilm inhibition was 65.68 ± 2.62% in E. coli and 79.66 ± 3.82% in S. aureus. Cell viability assay, exopolysaccharides quantification, and confocal laser scanning microscopy studies also revealed the enhanced antibiofilm activity of MG-MSN when used as a potential photosensitizer for aPDT. This study can be extended to eradicate these strains from localized superficial infections and medical appliances, preventing nosocomial infections.https://peerj.com/articles/7454.pdfaPDTBiofilmsCLSMMesoporous silica nanoparticleMG-MSN |
| spellingShingle | Parasuraman Paramanantham Busi Siddhardha Sruthil Lal SB Alok Sharan Abdullah A. Alyousef Mohammed Saeed Al Dosary Mohammed Arshad Asad Syed Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study PeerJ aPDT Biofilms CLSM Mesoporous silica nanoparticle MG-MSN |
| title | Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study |
| title_full | Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study |
| title_fullStr | Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study |
| title_full_unstemmed | Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study |
| title_short | Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study |
| title_sort | antimicrobial photodynamic therapy on staphylococcus aureus and escherichia coli using malachite green encapsulated mesoporous silica nanoparticles an in vitro study |
| topic | aPDT Biofilms CLSM Mesoporous silica nanoparticle MG-MSN |
| url | https://peerj.com/articles/7454.pdf |
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