Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems
For the first time AFM (atomic-force microscopy) was used to record significant changes in the geometric parameters of the image of erythrocytes in vitro under conditions of glycolytic starvation (ATP (Adenosine triphosphate) deficiency). The difference in the action of antioxidants, phenosan K, and...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Micro |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8023/3/2/26 |
| _version_ | 1797593540069425152 |
|---|---|
| author | Vladimir Binyukov Elena Mil Ludmila Matienko Anastasia Albantova Alexander Goloshchapov |
| author_facet | Vladimir Binyukov Elena Mil Ludmila Matienko Anastasia Albantova Alexander Goloshchapov |
| author_sort | Vladimir Binyukov |
| collection | DOAJ |
| description | For the first time AFM (atomic-force microscopy) was used to record significant changes in the geometric parameters of the image of erythrocytes in vitro under conditions of glycolytic starvation (ATP (Adenosine triphosphate) deficiency). The difference in the action of antioxidants, phenosan K, and Ihfan-10 on erythrocytes that we detected with AFM seems to be mainly due to their difference in hydrophobicity. We used the AFM method to research the self-organization of the components of the active center of P450 (Porphyrin-450) metalloenzymes that are part of a class of hemoproteins with functions of affinity to molecular oxygen O<sub>2</sub>. Stable supramolecular nanostructures in the form of triangular prisms based on the iron porphyrin complex with amino acids due to self-assembly involving intermolecular hydrogen bonds were received. A possible scheme for the formation of such structures is proposed. |
| first_indexed | 2024-03-11T02:09:40Z |
| format | Article |
| id | doaj.art-c35791b453554dca8fd5d5dc5d35cdae |
| institution | Directory Open Access Journal |
| issn | 2673-8023 |
| language | English |
| last_indexed | 2024-03-11T02:09:40Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micro |
| spelling | doaj.art-c35791b453554dca8fd5d5dc5d35cdae2023-11-18T11:37:45ZengMDPI AGMicro2673-80232023-03-013238239010.3390/micro3020026Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model SystemsVladimir Binyukov0Elena Mil1Ludmila Matienko2Anastasia Albantova3Alexander Goloshchapov4Emanuel Institute of Biochemical Physics Russian Academy of Science, 4 Kosygin Street, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics Russian Academy of Science, 4 Kosygin Street, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics Russian Academy of Science, 4 Kosygin Street, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics Russian Academy of Science, 4 Kosygin Street, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics Russian Academy of Science, 4 Kosygin Street, 119334 Moscow, RussiaFor the first time AFM (atomic-force microscopy) was used to record significant changes in the geometric parameters of the image of erythrocytes in vitro under conditions of glycolytic starvation (ATP (Adenosine triphosphate) deficiency). The difference in the action of antioxidants, phenosan K, and Ihfan-10 on erythrocytes that we detected with AFM seems to be mainly due to their difference in hydrophobicity. We used the AFM method to research the self-organization of the components of the active center of P450 (Porphyrin-450) metalloenzymes that are part of a class of hemoproteins with functions of affinity to molecular oxygen O<sub>2</sub>. Stable supramolecular nanostructures in the form of triangular prisms based on the iron porphyrin complex with amino acids due to self-assembly involving intermolecular hydrogen bonds were received. A possible scheme for the formation of such structures is proposed.https://www.mdpi.com/2673-8023/3/2/26AFMerythrocytesglucoseATP deficiencyphenolic antioxidantsnanostructures |
| spellingShingle | Vladimir Binyukov Elena Mil Ludmila Matienko Anastasia Albantova Alexander Goloshchapov Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems Micro AFM erythrocytes glucose ATP deficiency phenolic antioxidants nanostructures |
| title | Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems |
| title_full | Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems |
| title_fullStr | Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems |
| title_full_unstemmed | Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems |
| title_short | Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems |
| title_sort | methodic approach of atomic force microscopy afm to study morphological changes of cells and model systems |
| topic | AFM erythrocytes glucose ATP deficiency phenolic antioxidants nanostructures |
| url | https://www.mdpi.com/2673-8023/3/2/26 |
| work_keys_str_mv | AT vladimirbinyukov methodicapproachofatomicforcemicroscopyafmtostudymorphologicalchangesofcellsandmodelsystems AT elenamil methodicapproachofatomicforcemicroscopyafmtostudymorphologicalchangesofcellsandmodelsystems AT ludmilamatienko methodicapproachofatomicforcemicroscopyafmtostudymorphologicalchangesofcellsandmodelsystems AT anastasiaalbantova methodicapproachofatomicforcemicroscopyafmtostudymorphologicalchangesofcellsandmodelsystems AT alexandergoloshchapov methodicapproachofatomicforcemicroscopyafmtostudymorphologicalchangesofcellsandmodelsystems |