The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum
Fusarium oxysporum is one of the most important pathogenic fungi with a broad range of plant and animal hosts. The first key step of its infection cycle is conidial germination, but there is limited information available on the molecular events supporting this process. We show here that germination...
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| Format: | Article |
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eLife Sciences Publications Ltd
2021-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/75583 |
| _version_ | 1828382475057889280 |
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| author | Ning Zhang Limin Song Yang Xu Xueyuan Pei Ben F Luisi Wenxing Liang |
| author_facet | Ning Zhang Limin Song Yang Xu Xueyuan Pei Ben F Luisi Wenxing Liang |
| author_sort | Ning Zhang |
| collection | DOAJ |
| description | Fusarium oxysporum is one of the most important pathogenic fungi with a broad range of plant and animal hosts. The first key step of its infection cycle is conidial germination, but there is limited information available on the molecular events supporting this process. We show here that germination is accompanied by a sharp decrease in expression of FoSir5, an ortholog of the human lysine deacetylase SIRT5. We observe that FoSir5 decrotonylates a subunit of the fungal pyruvate dehydrogenase complex (FoDLAT) at K148, resulting in inhibition of the activity of the complex in mitochondria. Moreover, FoSir5 decrotonylates histone H3K18, leading to a downregulation of transcripts encoding enzymes of aerobic respiration pathways. Thus, the activity of FoSir5 coordinates regulation in different organelles to steer metabolic flux through respiration. As ATP content is positively related to fungal germination, we propose that FoSir5 negatively modulates conidial germination in F. oxysporum through its metabolic impact. These findings provide insights into the multifaceted roles of decrotonylation, catalyzed by FoSir5, that support conidial germination in F. oxysporum. |
| first_indexed | 2024-12-10T04:32:42Z |
| format | Article |
| id | doaj.art-c357a979c4bd47d88f820511c5ab91e1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T04:32:42Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-c357a979c4bd47d88f820511c5ab91e12022-12-22T02:02:06ZengeLife Sciences Publications LtdeLife2050-084X2021-12-011010.7554/eLife.75583The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporumNing Zhang0https://orcid.org/0000-0002-4824-3795Limin Song1Yang Xu2Xueyuan Pei3Ben F Luisi4https://orcid.org/0000-0003-1144-9877Wenxing Liang5https://orcid.org/0000-0002-3791-4901Shandong Province Key Laboratory of Applied Mycology, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, ChinaShandong Province Key Laboratory of Applied Mycology, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, ChinaShandong Province Key Laboratory of Applied Mycology, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, ChinaDepartment of Biochemistry, University of Cambridge, Cambridge, United KingdomDepartment of Biochemistry, University of Cambridge, Cambridge, United KingdomShandong Province Key Laboratory of Applied Mycology, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, ChinaFusarium oxysporum is one of the most important pathogenic fungi with a broad range of plant and animal hosts. The first key step of its infection cycle is conidial germination, but there is limited information available on the molecular events supporting this process. We show here that germination is accompanied by a sharp decrease in expression of FoSir5, an ortholog of the human lysine deacetylase SIRT5. We observe that FoSir5 decrotonylates a subunit of the fungal pyruvate dehydrogenase complex (FoDLAT) at K148, resulting in inhibition of the activity of the complex in mitochondria. Moreover, FoSir5 decrotonylates histone H3K18, leading to a downregulation of transcripts encoding enzymes of aerobic respiration pathways. Thus, the activity of FoSir5 coordinates regulation in different organelles to steer metabolic flux through respiration. As ATP content is positively related to fungal germination, we propose that FoSir5 negatively modulates conidial germination in F. oxysporum through its metabolic impact. These findings provide insights into the multifaceted roles of decrotonylation, catalyzed by FoSir5, that support conidial germination in F. oxysporum.https://elifesciences.org/articles/75583conidial germinaitonlysine crotonylationphytopathogenic fungiATP metabolism |
| spellingShingle | Ning Zhang Limin Song Yang Xu Xueyuan Pei Ben F Luisi Wenxing Liang The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum eLife conidial germinaiton lysine crotonylation phytopathogenic fungi ATP metabolism |
| title | The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum |
| title_full | The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum |
| title_fullStr | The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum |
| title_full_unstemmed | The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum |
| title_short | The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum |
| title_sort | decrotonylase fosir5 facilitates mitochondrial metabolic state switching in conidial germination of fusarium oxysporum |
| topic | conidial germinaiton lysine crotonylation phytopathogenic fungi ATP metabolism |
| url | https://elifesciences.org/articles/75583 |
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