Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil
Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (<i>Citrus sinensis</i> L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1371 |
| _version_ | 1797609454488780800 |
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| author | Camila Chabi-Jesus Pedro Luis Ramos-González Aline Daniele Tassi Laura Rossetto Pereira Marinês Bastianel Douglas Lau Maria Cristina Canale Ricardo Harakava Valdenice Moreira Novelli Elliot Watanabe Kitajima Juliana Freitas-Astúa |
| author_facet | Camila Chabi-Jesus Pedro Luis Ramos-González Aline Daniele Tassi Laura Rossetto Pereira Marinês Bastianel Douglas Lau Maria Cristina Canale Ricardo Harakava Valdenice Moreira Novelli Elliot Watanabe Kitajima Juliana Freitas-Astúa |
| author_sort | Camila Chabi-Jesus |
| collection | DOAJ |
| description | Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (<i>Citrus sinensis</i> L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(−)RNA viruses, with ORFs in a typical organization of members of the genus <i>Dichorhavirus</i>, were recovered. These genomes shared 98–99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by <i>Brevipalpus phoenicis</i> sensu stricto. In CiBSV-infected citrus plants, <i>B. papayensis</i> and <i>B. azores</i> were found, but the virus could only be transmitted to Arabidopsis plants by <i>B. azores.</i> The study provides the first evidence of the role of <i>B. azores</i> as a viral vector and supports the assignment of CiBSV to the tentative new species <i>Dichorhavirus australis</i>. |
| first_indexed | 2024-03-11T06:00:33Z |
| format | Article |
| id | doaj.art-c35c9a6a7b6a4f4498ff8f3fe445d600 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T06:00:33Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c35c9a6a7b6a4f4498ff8f3fe445d6002023-11-17T13:22:59ZengMDPI AGPlants2223-77472023-03-01126137110.3390/plants12061371Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in BrazilCamila Chabi-Jesus0Pedro Luis Ramos-González1Aline Daniele Tassi2Laura Rossetto Pereira3Marinês Bastianel4Douglas Lau5Maria Cristina Canale6Ricardo Harakava7Valdenice Moreira Novelli8Elliot Watanabe Kitajima9Juliana Freitas-Astúa10Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (Esalq/USP), Piracicaba 13418-900, São Paulo, BrazilInstituto Biológico/IB, São Paulo 04014-002, São Paulo, BrazilInstituto Biológico/IB, São Paulo 04014-002, São Paulo, BrazilInstituto Biológico/IB, São Paulo 04014-002, São Paulo, BrazilCentro de Citricultura Sylvio Moreira/IAC, Cordeirópolis 13490-970, São Paulo, BrazilEmbrapa Trigo, Passo Fundo 99050-970, Rio Grande do Sul, BrazilEmpresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina/Epagri, Paulo Lopes 88490-000, Santa Catarina, BrazilInstituto Biológico/IB, São Paulo 04014-002, São Paulo, BrazilCentro de Citricultura Sylvio Moreira/IAC, Cordeirópolis 13490-970, São Paulo, BrazilEscola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (Esalq/USP), Piracicaba 13418-900, São Paulo, BrazilInstituto Biológico/IB, São Paulo 04014-002, São Paulo, BrazilCitrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (<i>Citrus sinensis</i> L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(−)RNA viruses, with ORFs in a typical organization of members of the genus <i>Dichorhavirus</i>, were recovered. These genomes shared 98–99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by <i>Brevipalpus phoenicis</i> sensu stricto. In CiBSV-infected citrus plants, <i>B. papayensis</i> and <i>B. azores</i> were found, but the virus could only be transmitted to Arabidopsis plants by <i>B. azores.</i> The study provides the first evidence of the role of <i>B. azores</i> as a viral vector and supports the assignment of CiBSV to the tentative new species <i>Dichorhavirus australis</i>.https://www.mdpi.com/2223-7747/12/6/1371<i>Mononegavirales</i><i>Rhabdoviridae</i>vector mites |
| spellingShingle | Camila Chabi-Jesus Pedro Luis Ramos-González Aline Daniele Tassi Laura Rossetto Pereira Marinês Bastianel Douglas Lau Maria Cristina Canale Ricardo Harakava Valdenice Moreira Novelli Elliot Watanabe Kitajima Juliana Freitas-Astúa Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil Plants <i>Mononegavirales</i> <i>Rhabdoviridae</i> vector mites |
| title | Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil |
| title_full | Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil |
| title_fullStr | Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil |
| title_full_unstemmed | Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil |
| title_short | Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil |
| title_sort | citrus bright spot virus a new dichorhavirus transmitted by i brevipalpus azores i causing citrus leprosis disease in brazil |
| topic | <i>Mononegavirales</i> <i>Rhabdoviridae</i> vector mites |
| url | https://www.mdpi.com/2223-7747/12/6/1371 |
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