Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil
BACKGROUND The longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. Tocantins is the newest state of the Brazilian Federation. Despite a h...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2019-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100317&lng=en&tlng=en |
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| author | Eric Luiz Rodrigues de Sá Cynara de Melo Rodovalho Nilciane Pinto Ribeiro de Sousa Ivy Luizi Rodrigues de Sá Diogo Fernandes Bellinato Luciana dos Santos Dias Luana Carrara da Silva Ademir Jesus Martins José Bento Pereira Lima |
| author_facet | Eric Luiz Rodrigues de Sá Cynara de Melo Rodovalho Nilciane Pinto Ribeiro de Sousa Ivy Luizi Rodrigues de Sá Diogo Fernandes Bellinato Luciana dos Santos Dias Luana Carrara da Silva Ademir Jesus Martins José Bento Pereira Lima |
| author_sort | Eric Luiz Rodrigues de Sá |
| collection | DOAJ |
| description | BACKGROUND The longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. Tocantins is the newest state of the Brazilian Federation. Despite a historically high number of dengue cases, studies and monitoring data concerning insecticide resistance in the state are lacking. OBJECTIVES To verify the resistance status of Aedes aegypti from 10 populations distributed throughout the state connected by rivers and roads. METHODS Between 50 and 150 ovitraps were installed in house gardens within each municipality. Collection points were established based on the importance of the towns and on geographic aspects. Dose response bioassays were performed in accordance with World Health Organization guidelines. Molecular assays were conducted to detect kdr mutations, which are related to pyrethroid resistance. FINDINGS Of the 3,200 ovitrap paddles analysed, 25.8% contained eggs, with a total of 55,687 eggs collected. With the exception of Caseara, all evaluated populations were considered to be resistant to temephos. The data showed different levels of resistance to deltamethrin among the samples. Caseara and Guaraí showed the lowest RR95 values. On average, the NaVR1 kdr allele was most frequent (40.3%), followed by NaVS (38.1%), and NaVR2 (21.6%). Palmas, the capital of the state, had the highest frequency of kdr alleles (87.5%). MAIN CONCLUSIONS With the exception of Palmas, the towns with the highest indexes (ovitrap positivity, number and density of eggs), as well with high levels of resistance and kdr alleles were located along the BR-153 road, indicating that the flow of people and cargo can contribute to the dispersion of the vector and potentially resistance. This study contributes substantially to knowledge regarding the insecticide resistance profile of Tocantins mosquito populations; the data generated via the study could facilitate the judicious use of insecticides by vector control programs. |
| first_indexed | 2024-03-12T05:00:05Z |
| format | Article |
| id | doaj.art-c35e0550591841e6a6f30bc242ea65db |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:00:05Z |
| publishDate | 2019-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c35e0550591841e6a6f30bc242ea65db2023-09-03T09:14:08ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602019-03-01114010.1590/0074-02760180318S0074-02762019000100317Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in BrazilEric Luiz Rodrigues de SáCynara de Melo RodovalhoNilciane Pinto Ribeiro de SousaIvy Luizi Rodrigues de SáDiogo Fernandes BellinatoLuciana dos Santos DiasLuana Carrara da SilvaAdemir Jesus MartinsJosé Bento Pereira LimaBACKGROUND The longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. Tocantins is the newest state of the Brazilian Federation. Despite a historically high number of dengue cases, studies and monitoring data concerning insecticide resistance in the state are lacking. OBJECTIVES To verify the resistance status of Aedes aegypti from 10 populations distributed throughout the state connected by rivers and roads. METHODS Between 50 and 150 ovitraps were installed in house gardens within each municipality. Collection points were established based on the importance of the towns and on geographic aspects. Dose response bioassays were performed in accordance with World Health Organization guidelines. Molecular assays were conducted to detect kdr mutations, which are related to pyrethroid resistance. FINDINGS Of the 3,200 ovitrap paddles analysed, 25.8% contained eggs, with a total of 55,687 eggs collected. With the exception of Caseara, all evaluated populations were considered to be resistant to temephos. The data showed different levels of resistance to deltamethrin among the samples. Caseara and Guaraí showed the lowest RR95 values. On average, the NaVR1 kdr allele was most frequent (40.3%), followed by NaVS (38.1%), and NaVR2 (21.6%). Palmas, the capital of the state, had the highest frequency of kdr alleles (87.5%). MAIN CONCLUSIONS With the exception of Palmas, the towns with the highest indexes (ovitrap positivity, number and density of eggs), as well with high levels of resistance and kdr alleles were located along the BR-153 road, indicating that the flow of people and cargo can contribute to the dispersion of the vector and potentially resistance. This study contributes substantially to knowledge regarding the insecticide resistance profile of Tocantins mosquito populations; the data generated via the study could facilitate the judicious use of insecticides by vector control programs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100317&lng=en&tlng=enresistance monitoringarbovirusesAmazôniakdr mutation |
| spellingShingle | Eric Luiz Rodrigues de Sá Cynara de Melo Rodovalho Nilciane Pinto Ribeiro de Sousa Ivy Luizi Rodrigues de Sá Diogo Fernandes Bellinato Luciana dos Santos Dias Luana Carrara da Silva Ademir Jesus Martins José Bento Pereira Lima Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil Memorias do Instituto Oswaldo Cruz resistance monitoring arboviruses Amazônia kdr mutation |
| title | Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil |
| title_full | Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil |
| title_fullStr | Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil |
| title_full_unstemmed | Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil |
| title_short | Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil |
| title_sort | evaluation of insecticide resistance in aedes aegypti populations connected by roads and rivers the case of tocantins state in brazil |
| topic | resistance monitoring arboviruses Amazônia kdr mutation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100317&lng=en&tlng=en |
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