Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs
Genetic code expansion (GCE) can enable the site-selective incorporation of non-canonical amino acids (ncAAs) into proteins. GCE has advanced tremendously in the last decade and can be used to create biorthogonal handles, monitor and control proteins inside cells, study post-translational modificati...
| Main Authors: | , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2024-02-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.chimia.ch/chimia/article/view/7184 |
| _version_ | 1827339965891084288 |
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| author | Anton Natter Perdiguero Alexandria Deliz Liang |
| author_facet | Anton Natter Perdiguero Alexandria Deliz Liang |
| author_sort | Anton Natter Perdiguero |
| collection | DOAJ |
| description | Genetic code expansion (GCE) can enable the site-selective incorporation of non-canonical amino acids (ncAAs) into proteins. GCE has advanced tremendously in the last decade and can be used to create biorthogonal handles, monitor and control proteins inside cells, study post-translational modifications, and engineer new protein functions. Since establishing our laboratory, our research has focused on applications of GCE in protein and enzyme engineering using aminoacyl-tRNA synthetase/tRNA (aaRS/tRNA) pairs. This topic has been reviewed extensively, leaving little doubt that GCE is a powerful tool for engineering proteins and enzymes. Therefore, for this young faculty issue, we wanted to provide a more technical look into the methods we use and the challenges we think about in our laboratory. Since starting the laboratory, we have successfully engineered over a dozen novel aaRS/tRNA pairs tailored for various GCE applications. However, we acknowledge that the field can pose challenges even for experts. Thus, herein, we provide a review of methodologies in ncAA incorporation with some practical commentary and a focus on challenges, emerging solutions, and exciting developments.
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| first_indexed | 2024-03-07T20:09:16Z |
| format | Article |
| id | doaj.art-c35e5386d6fb4c75bcf4bf84d946c762 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-03-07T20:09:16Z |
| publishDate | 2024-02-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-c35e5386d6fb4c75bcf4bf84d946c7622024-02-28T02:07:50ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242024-02-01781/210.2533/chimia.2024.22Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA PairsAnton Natter Perdiguero0https://orcid.org/0009-0007-8775-9624Alexandria Deliz Liang1https://orcid.org/0000-0002-9897-2822Department of Chemistry, University of Zurich, Zurich, CH-8057 ZurichDepartment of Chemistry, University of Zurich, Zurich, CH-8057 ZurichGenetic code expansion (GCE) can enable the site-selective incorporation of non-canonical amino acids (ncAAs) into proteins. GCE has advanced tremendously in the last decade and can be used to create biorthogonal handles, monitor and control proteins inside cells, study post-translational modifications, and engineer new protein functions. Since establishing our laboratory, our research has focused on applications of GCE in protein and enzyme engineering using aminoacyl-tRNA synthetase/tRNA (aaRS/tRNA) pairs. This topic has been reviewed extensively, leaving little doubt that GCE is a powerful tool for engineering proteins and enzymes. Therefore, for this young faculty issue, we wanted to provide a more technical look into the methods we use and the challenges we think about in our laboratory. Since starting the laboratory, we have successfully engineered over a dozen novel aaRS/tRNA pairs tailored for various GCE applications. However, we acknowledge that the field can pose challenges even for experts. Thus, herein, we provide a review of methodologies in ncAA incorporation with some practical commentary and a focus on challenges, emerging solutions, and exciting developments. https://www.chimia.ch/chimia/article/view/7184Genetic code expansionNon-canonical amin acidsProtein engineeringUnnatural amino acids |
| spellingShingle | Anton Natter Perdiguero Alexandria Deliz Liang Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs CHIMIA Genetic code expansion Non-canonical amin acids Protein engineering Unnatural amino acids |
| title | Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs |
| title_full | Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs |
| title_fullStr | Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs |
| title_full_unstemmed | Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs |
| title_short | Practical Approaches to Genetic Code Expansion with Aminoacyl-tRNA Synthetase/tRNA Pairs |
| title_sort | practical approaches to genetic code expansion with aminoacyl trna synthetase trna pairs |
| topic | Genetic code expansion Non-canonical amin acids Protein engineering Unnatural amino acids |
| url | https://www.chimia.ch/chimia/article/view/7184 |
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