Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results
ObjectiveTo explore the effect of varying numbers of embryo washings prior to blastocyst formation in non-invasive preimplantation chromosome screening (NICS) on the accuracy of NICS results.MethodsIn this study, 68 blastocysts from preimplantation genetic testing (PGT)-assisted pregnancy were colle...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2024.1363851/full |
| _version_ | 1797245006958821376 |
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| author | Xiaomei Kang Meiting Wen Jie Zheng Fangxin Peng Ni Zeng Zhu Chen Yanting Wu Hong Sun |
| author_facet | Xiaomei Kang Meiting Wen Jie Zheng Fangxin Peng Ni Zeng Zhu Chen Yanting Wu Hong Sun |
| author_sort | Xiaomei Kang |
| collection | DOAJ |
| description | ObjectiveTo explore the effect of varying numbers of embryo washings prior to blastocyst formation in non-invasive preimplantation chromosome screening (NICS) on the accuracy of NICS results.MethodsIn this study, 68 blastocysts from preimplantation genetic testing (PGT)-assisted pregnancy were collected at our institution. On the fourth day of embryo culture, the embryos were transferred to a new medium for blastocyst culture and were washed either three times (NICS1 group) or ten times (NICS2 group). A trophectoderm (TE) biopsy was performed on the blastocysts, and the corresponding embryo culture media were collected for whole genome amplification (WGA) and high-throughput sequencing.ResultsThe success rate of WGA was 100% (TE biopsy), 76.7% (NICS1 group), and 89.5% (NICS2 group). The success rate of WGA in embryo medium on days 5 and 6 of culture was 75.0% (33/44) and 100% (24/24), respectively. Using TE as the gold standard, the karyotype concordance rate between the results of the NICS1 and NICS2 groups’ embryo culture medium samples and TE results was 43.5% (10/23) and 73.5% (25/34), respectively. The sensitivity and specificity of detecting chromosomal abnormalities were higher in the NICS2 group than in the NICS1 group when TE was used (83.3% vs 60.0%; 62.5% vs 30.8%, respectively). The false-positive rate and false-negative rate (i.e., misdiagnosis rate and missed diagnosis rate, respectively) were lower in the NICS2 group than in the NICS1 group (37.5% vs 69.2%; 16.7% vs 40.0%, respectively).ConclusionThe NICS yielded favorable results after ten washings of the embryos. These findings provide a novel method for lowering the amount of cell-free DNA contamination from non-embryonic sources in the medium used for embryo development, optimizing the sampling procedure and improving the accuracy of the NICS test. |
| first_indexed | 2024-04-24T19:20:03Z |
| format | Article |
| id | doaj.art-c35fa387ea124abfbd1fd8d117f64d20 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-24T19:20:03Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-c35fa387ea124abfbd1fd8d117f64d202024-03-26T04:24:59ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-03-011510.3389/fendo.2024.13638511363851Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening resultsXiaomei Kang0Meiting Wen1Jie Zheng2Fangxin Peng3Ni Zeng4Zhu Chen5Yanting Wu6Hong Sun7Reproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Zigong, Zigong, ChinaReproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaReproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaReproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaReproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaReproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaReproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, ChinaObjectiveTo explore the effect of varying numbers of embryo washings prior to blastocyst formation in non-invasive preimplantation chromosome screening (NICS) on the accuracy of NICS results.MethodsIn this study, 68 blastocysts from preimplantation genetic testing (PGT)-assisted pregnancy were collected at our institution. On the fourth day of embryo culture, the embryos were transferred to a new medium for blastocyst culture and were washed either three times (NICS1 group) or ten times (NICS2 group). A trophectoderm (TE) biopsy was performed on the blastocysts, and the corresponding embryo culture media were collected for whole genome amplification (WGA) and high-throughput sequencing.ResultsThe success rate of WGA was 100% (TE biopsy), 76.7% (NICS1 group), and 89.5% (NICS2 group). The success rate of WGA in embryo medium on days 5 and 6 of culture was 75.0% (33/44) and 100% (24/24), respectively. Using TE as the gold standard, the karyotype concordance rate between the results of the NICS1 and NICS2 groups’ embryo culture medium samples and TE results was 43.5% (10/23) and 73.5% (25/34), respectively. The sensitivity and specificity of detecting chromosomal abnormalities were higher in the NICS2 group than in the NICS1 group when TE was used (83.3% vs 60.0%; 62.5% vs 30.8%, respectively). The false-positive rate and false-negative rate (i.e., misdiagnosis rate and missed diagnosis rate, respectively) were lower in the NICS2 group than in the NICS1 group (37.5% vs 69.2%; 16.7% vs 40.0%, respectively).ConclusionThe NICS yielded favorable results after ten washings of the embryos. These findings provide a novel method for lowering the amount of cell-free DNA contamination from non-embryonic sources in the medium used for embryo development, optimizing the sampling procedure and improving the accuracy of the NICS test.https://www.frontiersin.org/articles/10.3389/fendo.2024.1363851/fullnon-invasive chromosome screeningfree chromosomeembryoassisted reproductive technology (ART)embryo washing |
| spellingShingle | Xiaomei Kang Meiting Wen Jie Zheng Fangxin Peng Ni Zeng Zhu Chen Yanting Wu Hong Sun Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results Frontiers in Endocrinology non-invasive chromosome screening free chromosome embryo assisted reproductive technology (ART) embryo washing |
| title | Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results |
| title_full | Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results |
| title_fullStr | Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results |
| title_full_unstemmed | Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results |
| title_short | Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results |
| title_sort | influence of the number of washings for embryos on non invasive preimplantation chromosome screening results |
| topic | non-invasive chromosome screening free chromosome embryo assisted reproductive technology (ART) embryo washing |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2024.1363851/full |
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