Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections
Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/4/596 |
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| author | Karin Stiasny Stefan Malafa Stephan W. Aberle Iris Medits Georgios Tsouchnikas Judith H. Aberle Heidemarie Holzmann Franz X. Heinz |
| author_facet | Karin Stiasny Stefan Malafa Stephan W. Aberle Iris Medits Georgios Tsouchnikas Judith H. Aberle Heidemarie Holzmann Franz X. Heinz |
| author_sort | Karin Stiasny |
| collection | DOAJ |
| description | Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviviruses. Therefore, serological diagnosis of a recent infection can be insufficiently specific, especially in areas where flaviviruses co-circulate and/or vaccination coverage against certain flaviviruses is high. In this study, we developed a new IgM assay format, which is well suited for the specific diagnosis of TBE, Zika and dengue virus infections. In the case of TBE and Zika, the IgM response proved to be highly specific for the infecting virus. In contrast, primary dengue virus infections induced substantial amounts of cross-reactive IgM antibodies, which is most likely explained by structural peculiarities of dengue virus particles. Despite the presence of cross-reactive IgM, the standardized nature and the quantitative read-out of the assay even allowed the serotype-specific diagnosis of recent dengue virus infections in most instances. |
| first_indexed | 2024-03-10T12:43:50Z |
| format | Article |
| id | doaj.art-c35fc201aca4420f9c6409536d64866d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T12:43:50Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c35fc201aca4420f9c6409536d64866d2023-11-21T13:38:47ZengMDPI AGViruses1999-49152021-03-0113459610.3390/v13040596Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus InfectionsKarin Stiasny0Stefan Malafa1Stephan W. Aberle2Iris Medits3Georgios Tsouchnikas4Judith H. Aberle5Heidemarie Holzmann6Franz X. Heinz7Center for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaCenter for Virology, Medical University of Vienna, 1090 Vienna, AustriaFlaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviviruses. Therefore, serological diagnosis of a recent infection can be insufficiently specific, especially in areas where flaviviruses co-circulate and/or vaccination coverage against certain flaviviruses is high. In this study, we developed a new IgM assay format, which is well suited for the specific diagnosis of TBE, Zika and dengue virus infections. In the case of TBE and Zika, the IgM response proved to be highly specific for the infecting virus. In contrast, primary dengue virus infections induced substantial amounts of cross-reactive IgM antibodies, which is most likely explained by structural peculiarities of dengue virus particles. Despite the presence of cross-reactive IgM, the standardized nature and the quantitative read-out of the assay even allowed the serotype-specific diagnosis of recent dengue virus infections in most instances.https://www.mdpi.com/1999-4915/13/4/596flavivirus cross-reactivityflavivirus IgM responsedengueZikatick-borne encephalitis |
| spellingShingle | Karin Stiasny Stefan Malafa Stephan W. Aberle Iris Medits Georgios Tsouchnikas Judith H. Aberle Heidemarie Holzmann Franz X. Heinz Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections Viruses flavivirus cross-reactivity flavivirus IgM response dengue Zika tick-borne encephalitis |
| title | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
| title_full | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
| title_fullStr | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
| title_full_unstemmed | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
| title_short | Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections |
| title_sort | different cross reactivities of igm responses in dengue zika and tick borne encephalitis virus infections |
| topic | flavivirus cross-reactivity flavivirus IgM response dengue Zika tick-borne encephalitis |
| url | https://www.mdpi.com/1999-4915/13/4/596 |
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