A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell
In many cell types, lateral diffusion barriers compartmentalize the plasma membrane and, at least in budding yeast, the endoplasmic reticulum (ER). However, the molecular nature of these barriers, their mode of action and their cellular functions are unclear. Here, we show that misfolded proteins of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01883 |
| _version_ | 1811201209089916928 |
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| author | Lori Clay Fabrice Caudron Annina Denoth-Lippuner Barbara Boettcher Stéphanie Buvelot Frei Erik Lee Snapp Yves Barral |
| author_facet | Lori Clay Fabrice Caudron Annina Denoth-Lippuner Barbara Boettcher Stéphanie Buvelot Frei Erik Lee Snapp Yves Barral |
| author_sort | Lori Clay |
| collection | DOAJ |
| description | In many cell types, lateral diffusion barriers compartmentalize the plasma membrane and, at least in budding yeast, the endoplasmic reticulum (ER). However, the molecular nature of these barriers, their mode of action and their cellular functions are unclear. Here, we show that misfolded proteins of the ER remain confined into the mother compartment of budding yeast cells. Confinement required the formation of a lateral diffusion barrier in the form of a distinct domain of the ER-membrane at the bud neck, in a septin-, Bud1 GTPase- and sphingolipid-dependent manner. The sphingolipids, but not Bud1, also contributed to barrier formation in the outer membrane of the dividing nucleus. Barrier-dependent confinement of ER stress into the mother cell promoted aging. Together, our data clarify the physical nature of lateral diffusion barriers in the ER and establish the role of such barriers in the asymmetric segregation of proteotoxic misfolded proteins during cell division and aging. |
| first_indexed | 2024-04-12T02:18:21Z |
| format | Article |
| id | doaj.art-c36063efd9a043feb201d8d8de75869c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:18:21Z |
| publishDate | 2014-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c36063efd9a043feb201d8d8de75869c2022-12-22T03:52:12ZengeLife Sciences Publications LtdeLife2050-084X2014-05-01310.7554/eLife.01883A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cellLori Clay0Fabrice Caudron1Annina Denoth-Lippuner2Barbara Boettcher3Stéphanie Buvelot Frei4Erik Lee Snapp5Yves Barral6Institute of Biochemistry, Department of Biology, ETH Zürich, Zürich, SwitzerlandInstitute of Biochemistry, Department of Biology, ETH Zürich, Zürich, SwitzerlandInstitute of Biochemistry, Department of Biology, ETH Zürich, Zürich, SwitzerlandInstitute of Biochemistry, Department of Biology, ETH Zürich, Zürich, SwitzerlandInstitute of Biochemistry, Department of Biology, ETH Zürich, Zürich, SwitzerlandDepartment of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, New York, United StatesInstitute of Biochemistry, Department of Biology, ETH Zürich, Zürich, SwitzerlandIn many cell types, lateral diffusion barriers compartmentalize the plasma membrane and, at least in budding yeast, the endoplasmic reticulum (ER). However, the molecular nature of these barriers, their mode of action and their cellular functions are unclear. Here, we show that misfolded proteins of the ER remain confined into the mother compartment of budding yeast cells. Confinement required the formation of a lateral diffusion barrier in the form of a distinct domain of the ER-membrane at the bud neck, in a septin-, Bud1 GTPase- and sphingolipid-dependent manner. The sphingolipids, but not Bud1, also contributed to barrier formation in the outer membrane of the dividing nucleus. Barrier-dependent confinement of ER stress into the mother cell promoted aging. Together, our data clarify the physical nature of lateral diffusion barriers in the ER and establish the role of such barriers in the asymmetric segregation of proteotoxic misfolded proteins during cell division and aging.https://elifesciences.org/articles/01883diffusion barriersphingolipidER stressagingasymmetric cell division |
| spellingShingle | Lori Clay Fabrice Caudron Annina Denoth-Lippuner Barbara Boettcher Stéphanie Buvelot Frei Erik Lee Snapp Yves Barral A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell eLife diffusion barrier sphingolipid ER stress aging asymmetric cell division |
| title | A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell |
| title_full | A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell |
| title_fullStr | A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell |
| title_full_unstemmed | A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell |
| title_short | A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell |
| title_sort | sphingolipid dependent diffusion barrier confines er stress to the yeast mother cell |
| topic | diffusion barrier sphingolipid ER stress aging asymmetric cell division |
| url | https://elifesciences.org/articles/01883 |
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