Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers
Abstract Background Oleaginous yeasts are promising microbial platforms for sustainable, bio-based production of biofuels and oleochemical building blocks. Bio-based residues provide sustainable and cost-effective carbon sources for fermentative yeast oil production without land-use change. Consider...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-021-01710-3 |
| _version_ | 1818701315978559488 |
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| author | Pariya Shaigani Dania Awad Veronika Redai Monika Fuchs Martina Haack Norbert Mehlmer Thomas Brueck |
| author_facet | Pariya Shaigani Dania Awad Veronika Redai Monika Fuchs Martina Haack Norbert Mehlmer Thomas Brueck |
| author_sort | Pariya Shaigani |
| collection | DOAJ |
| description | Abstract Background Oleaginous yeasts are promising microbial platforms for sustainable, bio-based production of biofuels and oleochemical building blocks. Bio-based residues provide sustainable and cost-effective carbon sources for fermentative yeast oil production without land-use change. Considering the regional abundancy of different waste streams, we chose complex biomass residue streams of marine origin; macroalgae hydrolysate, and terrestrial origin; wheat straw hydrolysate in the presence, and absence of corn steep liquor as a complex nitrogen source. We investigated the biomass and lipid yields of an array of well-described oleaginous yeasts; R. glutinis, T. asahii, R. mucilaginosa, R. toruloides, C. oleaginosus growing on these hydrolysates. Furthermore, their sugar utilization, fatty acid profile, and inhibitory effect of the hydrolysates on yeast growth were compared. For correlative reference, we initially performed comparative growth experiments for the strains on individual monomeric sugars separately. Each of these monomeric sugars was a dominant carbon source in the complex biomass hydrolysates evaluated in this study. In addition, we evaluated N-acetylglucosamine, the monomeric building block of chitin, as a low-cost nitrogen and carbon source in yeast fermentation. Results C. oleaginosus provided the highest biomass and lipid yields. In the wheat straw and brown algae hydrolysates, this yeast strain gained 7.5 g/L and 3.8 g/L lipids, respectively. Cultivation in algae hydrolysate resulted in a higher level of unsaturated fatty acids in the lipids accumulated by all yeast strains. R. toruloides and C. oleaginosus were able to effectively co-utilize mannitol, glucose, and xylose. Growth rates on wheat straw hydrolysate were enhanced in presence of corn steep liquor. Conclusions Among the yeast strains investigated in this study, C. oleaginosus proved to be the most versatile strain in terms of substrate utilization, productivity, and tolerance in the complex media. Various fatty acid profiles obtained on each substrate encourage the manipulation of culture conditions to achieve the desired fatty acid composition for each application. This could be accomplished by combining the element of carbon source with other formerly studied factors such as temperature and oxygen. Moreover, corn steep liquor showed promise for enhancement of growth in the oleaginous strains provided that carbon substrate is available. |
| first_indexed | 2024-12-17T15:18:54Z |
| format | Article |
| id | doaj.art-c361a473d54842ebb0006b6dfbf7b83a |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-17T15:18:54Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-c361a473d54842ebb0006b6dfbf7b83a2022-12-21T21:43:27ZengBMCMicrobial Cell Factories1475-28592021-12-0120111810.1186/s12934-021-01710-3Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producersPariya Shaigani0Dania Awad1Veronika Redai2Monika Fuchs3Martina Haack4Norbert Mehlmer5Thomas Brueck6Werner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichWerner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichWerner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichWerner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichWerner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichWerner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichWerner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of MunichAbstract Background Oleaginous yeasts are promising microbial platforms for sustainable, bio-based production of biofuels and oleochemical building blocks. Bio-based residues provide sustainable and cost-effective carbon sources for fermentative yeast oil production without land-use change. Considering the regional abundancy of different waste streams, we chose complex biomass residue streams of marine origin; macroalgae hydrolysate, and terrestrial origin; wheat straw hydrolysate in the presence, and absence of corn steep liquor as a complex nitrogen source. We investigated the biomass and lipid yields of an array of well-described oleaginous yeasts; R. glutinis, T. asahii, R. mucilaginosa, R. toruloides, C. oleaginosus growing on these hydrolysates. Furthermore, their sugar utilization, fatty acid profile, and inhibitory effect of the hydrolysates on yeast growth were compared. For correlative reference, we initially performed comparative growth experiments for the strains on individual monomeric sugars separately. Each of these monomeric sugars was a dominant carbon source in the complex biomass hydrolysates evaluated in this study. In addition, we evaluated N-acetylglucosamine, the monomeric building block of chitin, as a low-cost nitrogen and carbon source in yeast fermentation. Results C. oleaginosus provided the highest biomass and lipid yields. In the wheat straw and brown algae hydrolysates, this yeast strain gained 7.5 g/L and 3.8 g/L lipids, respectively. Cultivation in algae hydrolysate resulted in a higher level of unsaturated fatty acids in the lipids accumulated by all yeast strains. R. toruloides and C. oleaginosus were able to effectively co-utilize mannitol, glucose, and xylose. Growth rates on wheat straw hydrolysate were enhanced in presence of corn steep liquor. Conclusions Among the yeast strains investigated in this study, C. oleaginosus proved to be the most versatile strain in terms of substrate utilization, productivity, and tolerance in the complex media. Various fatty acid profiles obtained on each substrate encourage the manipulation of culture conditions to achieve the desired fatty acid composition for each application. This could be accomplished by combining the element of carbon source with other formerly studied factors such as temperature and oxygen. Moreover, corn steep liquor showed promise for enhancement of growth in the oleaginous strains provided that carbon substrate is available.https://doi.org/10.1186/s12934-021-01710-3Oleaginous yeastsCarbon substrate preferenceFermentation inhibitor toleranceBiomass yieldLipid yieldComplex lignocellulosic/marine biomass hydrolysate |
| spellingShingle | Pariya Shaigani Dania Awad Veronika Redai Monika Fuchs Martina Haack Norbert Mehlmer Thomas Brueck Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers Microbial Cell Factories Oleaginous yeasts Carbon substrate preference Fermentation inhibitor tolerance Biomass yield Lipid yield Complex lignocellulosic/marine biomass hydrolysate |
| title | Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers |
| title_full | Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers |
| title_fullStr | Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers |
| title_full_unstemmed | Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers |
| title_short | Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers |
| title_sort | oleaginous yeasts substrate preference and lipid productivity a view on the performance of microbial lipid producers |
| topic | Oleaginous yeasts Carbon substrate preference Fermentation inhibitor tolerance Biomass yield Lipid yield Complex lignocellulosic/marine biomass hydrolysate |
| url | https://doi.org/10.1186/s12934-021-01710-3 |
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