ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR.
Approximately 3-7% of non-small cell lung cancers harbor an anaplastic lymphoma kinase (ALK) gene fusion, constituting a new molecular subtype of lung cancer that responds to crizotinib, an ALK inhibitor. Although previous studies have evaluated ALK-rearranged lung cancers, the comprehensive analysi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3724879?pdf=render |
| _version_ | 1818580447576195072 |
|---|---|
| author | Yuan Li Yunjian Pan Rui Wang Yihua Sun Haichuan Hu Xuxia Shen Yongming Lu Lei Shen Xiongzeng Zhu Haiquan Chen |
| author_facet | Yuan Li Yunjian Pan Rui Wang Yihua Sun Haichuan Hu Xuxia Shen Yongming Lu Lei Shen Xiongzeng Zhu Haiquan Chen |
| author_sort | Yuan Li |
| collection | DOAJ |
| description | Approximately 3-7% of non-small cell lung cancers harbor an anaplastic lymphoma kinase (ALK) gene fusion, constituting a new molecular subtype of lung cancer that responds to crizotinib, an ALK inhibitor. Although previous studies have evaluated ALK-rearranged lung cancers, the comprehensive analysis of lung cancer in Chinese has not well assessed. Herein, we identified 44 cases of ALK-rearranged samples by fluorescent in-situ hybridization (FISH), immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) in a large number of surgically resected lung cancers. All 44 ALK-rearranged lung cancers were adenocarcinomas, with 2 cases having additional focal squamous components. The goal was to analyse the clinicopathological features of ALK-rearranged lung adenocarcinomas. Our data showed that a cribriform structure, prominent extracellular mucus and any type of mucous cell pattern may be either sensitive or specific to predict an ALK rearrangement. We used FISH as the standard detection method. We compared the ALK rearrangement accuracy of FISH, RT-PCR and IHC. RT-PCR could define both the ALK fusion partner and the fusion variant, but seemed unable to detect all translocations involving the ALK gene. It is noteworthy that IHC using the D5F3 antibody (Cell Signaling Technology) showed higher sensitivity and specificity than the ALK1 antibody (Dako). Therefore, we conclude that IHC remains a cost-effective and efficient technique for diagnosing ALK rearrangements and that D5F3 can be the optimal screening antibody in clinical practice. |
| first_indexed | 2024-12-16T07:17:45Z |
| format | Article |
| id | doaj.art-c3662c4ab0d04a45bdbe260c0026ac9f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T07:17:45Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c3662c4ab0d04a45bdbe260c0026ac9f2022-12-21T22:39:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6901610.1371/journal.pone.0069016ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR.Yuan LiYunjian PanRui WangYihua SunHaichuan HuXuxia ShenYongming LuLei ShenXiongzeng ZhuHaiquan ChenApproximately 3-7% of non-small cell lung cancers harbor an anaplastic lymphoma kinase (ALK) gene fusion, constituting a new molecular subtype of lung cancer that responds to crizotinib, an ALK inhibitor. Although previous studies have evaluated ALK-rearranged lung cancers, the comprehensive analysis of lung cancer in Chinese has not well assessed. Herein, we identified 44 cases of ALK-rearranged samples by fluorescent in-situ hybridization (FISH), immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) in a large number of surgically resected lung cancers. All 44 ALK-rearranged lung cancers were adenocarcinomas, with 2 cases having additional focal squamous components. The goal was to analyse the clinicopathological features of ALK-rearranged lung adenocarcinomas. Our data showed that a cribriform structure, prominent extracellular mucus and any type of mucous cell pattern may be either sensitive or specific to predict an ALK rearrangement. We used FISH as the standard detection method. We compared the ALK rearrangement accuracy of FISH, RT-PCR and IHC. RT-PCR could define both the ALK fusion partner and the fusion variant, but seemed unable to detect all translocations involving the ALK gene. It is noteworthy that IHC using the D5F3 antibody (Cell Signaling Technology) showed higher sensitivity and specificity than the ALK1 antibody (Dako). Therefore, we conclude that IHC remains a cost-effective and efficient technique for diagnosing ALK rearrangements and that D5F3 can be the optimal screening antibody in clinical practice.http://europepmc.org/articles/PMC3724879?pdf=render |
| spellingShingle | Yuan Li Yunjian Pan Rui Wang Yihua Sun Haichuan Hu Xuxia Shen Yongming Lu Lei Shen Xiongzeng Zhu Haiquan Chen ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR. PLoS ONE |
| title | ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR. |
| title_full | ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR. |
| title_fullStr | ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR. |
| title_full_unstemmed | ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR. |
| title_short | ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR. |
| title_sort | alk rearranged lung cancer in chinese a comprehensive assessment of clinicopathology ihc fish and rt pcr |
| url | http://europepmc.org/articles/PMC3724879?pdf=render |
| work_keys_str_mv | AT yuanli alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT yunjianpan alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT ruiwang alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT yihuasun alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT haichuanhu alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT xuxiashen alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT yongminglu alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT leishen alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT xiongzengzhu alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr AT haiquanchen alkrearrangedlungcancerinchineseacomprehensiveassessmentofclinicopathologyihcfishandrtpcr |