COLD-PCR amplification of bisulfite-converted DNA allows the enrichment and sequencing of rare un-methylated genomic regions.

COLD-PCR amplification of bisulfite-converted DNA allows the enrichment and sequencing of rare un-methylated genomic regions.

Aberrant hypo-methylation of DNA is evident in a range of human diseases including cancer and diabetes. Development of sensitive assays capable of detecting traces of un-methylated DNA within methylated samples can be useful in several situations. Here we describe a new approach, fast-COLD-MS-PCR, w...

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Bibliographic Details
Main Authors: Elena Castellanos-Rizaldos, Coren A Milbury, Elli Karatza, Clark C Chen, G Mike Makrigiorgos, Anne Merewood
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094103&type=printable

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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094103&type=printable

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