Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE
A new method is described for the quantitative detection of IgE antibodies, based on IgE capture with a specific antibody, reaction with liquid-biotinylated allergens and biotinylated anti-IgE and immunoenzymatic development of the reaction (reverse enzyme allergosorbent test). Using a reference sys...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1999-01-01
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| Series: | Allergology International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015314854 |
| _version_ | 1818179490461777920 |
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| author | Paolo Falagiani Gianni Mistrello Giuseppina Rapisarda Graziella Riva Daniela Roncarolo Dario Zanoni Susanna Voltolini Emanuele Crimi |
| author_facet | Paolo Falagiani Gianni Mistrello Giuseppina Rapisarda Graziella Riva Daniela Roncarolo Dario Zanoni Susanna Voltolini Emanuele Crimi |
| author_sort | Paolo Falagiani |
| collection | DOAJ |
| description | A new method is described for the quantitative detection of IgE antibodies, based on IgE capture with a specific antibody, reaction with liquid-biotinylated allergens and biotinylated anti-IgE and immunoenzymatic development of the reaction (reverse enzyme allergosorbent test). Using a reference system based on the World Health Organization IgE International standard, this method determines total IgE in the range 2-100kU/L and specific IgE in the range 0.2-100 kU/L, from which the specific/total ratio, called 'specific IgE density', can be calculated. This procedure has been applied to the study of specific IgE in 23 sera from patients polysensitized to pollen and mite allergens: 11 with asthma and 12 with rhinitis. The sensitivity and reproducibility of the method were evaluted. Sera from asthmatic patients showed higher cumulative levels of specific IgE (mean density 57.7%) than sera from rhinitic patients (mean density 32.6%). The clinical significance of specific IgE density in patients with multiple sensitizations is discussed. |
| first_indexed | 2024-12-11T21:04:42Z |
| format | Article |
| id | doaj.art-c36ab4bbbc784f01bd5d54bf7438b241 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-11T21:04:42Z |
| publishDate | 1999-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-c36ab4bbbc784f01bd5d54bf7438b2412022-12-22T00:50:53ZengElsevierAllergology International1323-89301999-01-0148319920710.1046/j.1440-1592.1999.00135.xSpecific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgEPaolo Falagiani0Gianni Mistrello1Giuseppina Rapisarda2Graziella Riva3Daniela Roncarolo4Dario Zanoni5Susanna Voltolini6Emanuele Crimi7Research Department, Lofarma SpA, MilanResearch Department, Lofarma SpA, MilanResearch Department, Lofarma SpA, MilanResearch Department, Lofarma SpA, MilanResearch Department, Lofarma SpA, MilanResearch Department, Lofarma SpA, MilanServizio di Allergologia, Ospedale S. Martino, GenovaCattedra di Fisiopatologia Respiratoria, Dipartimento di Scienze Motorie e Riabilitative, Università di Genova, Genova, ItalyA new method is described for the quantitative detection of IgE antibodies, based on IgE capture with a specific antibody, reaction with liquid-biotinylated allergens and biotinylated anti-IgE and immunoenzymatic development of the reaction (reverse enzyme allergosorbent test). Using a reference system based on the World Health Organization IgE International standard, this method determines total IgE in the range 2-100kU/L and specific IgE in the range 0.2-100 kU/L, from which the specific/total ratio, called 'specific IgE density', can be calculated. This procedure has been applied to the study of specific IgE in 23 sera from patients polysensitized to pollen and mite allergens: 11 with asthma and 12 with rhinitis. The sensitivity and reproducibility of the method were evaluted. Sera from asthmatic patients showed higher cumulative levels of specific IgE (mean density 57.7%) than sera from rhinitic patients (mean density 32.6%). The clinical significance of specific IgE density in patients with multiple sensitizations is discussed.http://www.sciencedirect.com/science/article/pii/S1323893015314854allergensIgE densityradioallergosorbent testreverse enzyme allergosorbent testspecific IgEtotal IgE |
| spellingShingle | Paolo Falagiani Gianni Mistrello Giuseppina Rapisarda Graziella Riva Daniela Roncarolo Dario Zanoni Susanna Voltolini Emanuele Crimi Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE Allergology International allergens IgE density radioallergosorbent test reverse enzyme allergosorbent test specific IgE total IgE |
| title | Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE |
| title_full | Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE |
| title_fullStr | Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE |
| title_full_unstemmed | Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE |
| title_short | Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE |
| title_sort | specific ige density assay a new reverse enzyme allergosorbent test based procedure for the quantitative detection of allergen specific ige |
| topic | allergens IgE density radioallergosorbent test reverse enzyme allergosorbent test specific IgE total IgE |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015314854 |
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