Viroids: sequence variability and evolution of pathogenic RNA
Viroids as the smallest pathogenic circular single-stranded pathogenic RNAs form populations of quasi-species, whichhas been recently identified by thermodynamic methods like TGGE pre-selection and heteroduplex analysis. It was foundthat replication under thermal stress led to enormously high level...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-06-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200210-0041_viroids-sequence-variability-and-evolution-of-pathogenic-rna.php |
| _version_ | 1828007895751458816 |
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| author | J. Matoušek |
| author_facet | J. Matoušek |
| author_sort | J. Matoušek |
| collection | DOAJ |
| description | Viroids as the smallest pathogenic circular single-stranded pathogenic RNAs form populations of quasi-species, whichhas been recently identified by thermodynamic methods like TGGE pre-selection and heteroduplex analysis. It was foundthat replication under thermal stress led to enormously high level of viroid mutagenesis. Mostly multiple mutants havingnon-random distribution of base changes were found. A specific "hot spots" were identified in the regions, wherea characteristic "pathogenicity domains" are localised in different viroids of the pospiviroidae family. Specific viroidmicroevolution was observed upon artificial inoculation of non-host plant species. Our results suggest that viroid propagationunder physiological stress can be assumed as important factor, which is among others, responsible for an appearanceof viroid quasi-species in the nature. Evolution and new viroid patotypes could accumulate due to environmental stressincluding various pollutants may be a potential danger for cultured plants. |
| first_indexed | 2024-04-10T08:16:37Z |
| format | Article |
| id | doaj.art-c36c63bc278a41bf838381bc4ce24409 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:16:37Z |
| publishDate | 2002-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-c36c63bc278a41bf838381bc4ce244092023-02-23T03:43:40ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412002-06-0138SI 1 - 6th Conf EFPP17317610.17221/10348-PPSpps-200210-0041Viroids: sequence variability and evolution of pathogenic RNAJ. Matoušek0Institute of Plant Molecular Biology Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czech RepublicViroids as the smallest pathogenic circular single-stranded pathogenic RNAs form populations of quasi-species, whichhas been recently identified by thermodynamic methods like TGGE pre-selection and heteroduplex analysis. It was foundthat replication under thermal stress led to enormously high level of viroid mutagenesis. Mostly multiple mutants havingnon-random distribution of base changes were found. A specific "hot spots" were identified in the regions, wherea characteristic "pathogenicity domains" are localised in different viroids of the pospiviroidae family. Specific viroidmicroevolution was observed upon artificial inoculation of non-host plant species. Our results suggest that viroid propagationunder physiological stress can be assumed as important factor, which is among others, responsible for an appearanceof viroid quasi-species in the nature. Evolution and new viroid patotypes could accumulate due to environmental stressincluding various pollutants may be a potential danger for cultured plants.https://pps.agriculturejournals.cz/artkey/pps-200210-0041_viroids-sequence-variability-and-evolution-of-pathogenic-rna.phpviroid mutagenesisquasi-speciesdna heteroduplexesstress factorsviroid pathotypes |
| spellingShingle | J. Matoušek Viroids: sequence variability and evolution of pathogenic RNA Plant Protection Science viroid mutagenesis quasi-species dna heteroduplexes stress factors viroid pathotypes |
| title | Viroids: sequence variability and evolution of pathogenic RNA |
| title_full | Viroids: sequence variability and evolution of pathogenic RNA |
| title_fullStr | Viroids: sequence variability and evolution of pathogenic RNA |
| title_full_unstemmed | Viroids: sequence variability and evolution of pathogenic RNA |
| title_short | Viroids: sequence variability and evolution of pathogenic RNA |
| title_sort | viroids sequence variability and evolution of pathogenic rna |
| topic | viroid mutagenesis quasi-species dna heteroduplexes stress factors viroid pathotypes |
| url | https://pps.agriculturejournals.cz/artkey/pps-200210-0041_viroids-sequence-variability-and-evolution-of-pathogenic-rna.php |
| work_keys_str_mv | AT jmatousek viroidssequencevariabilityandevolutionofpathogenicrna |