Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities
The proteinaceous compounds produced by lactic acid bacteria are called bacteriocins and have a wide variety of bioactive properties. However, bacteriocin’s commercial availability is limited due to short stability periods and low yields. Therefore, the objective of this study was to synthesize bact...
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MDPI AG
2023-01-01
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| author | Arif Jamal Siddiqui Mitesh Patel Mohd Adnan Sadaf Jahan Juhi Saxena Mohammed Merae Alshahrani Abdelmushin Abdelgadir Fevzi Bardakci Manojkumar Sachidanandan Riadh Badraoui Mejdi Snoussi Allal Ouhtit |
| author_facet | Arif Jamal Siddiqui Mitesh Patel Mohd Adnan Sadaf Jahan Juhi Saxena Mohammed Merae Alshahrani Abdelmushin Abdelgadir Fevzi Bardakci Manojkumar Sachidanandan Riadh Badraoui Mejdi Snoussi Allal Ouhtit |
| author_sort | Arif Jamal Siddiqui |
| collection | DOAJ |
| description | The proteinaceous compounds produced by lactic acid bacteria are called bacteriocins and have a wide variety of bioactive properties. However, bacteriocin’s commercial availability is limited due to short stability periods and low yields. Therefore, the objective of this study was to synthesize bacteriocin-derived silver nanoparticles (Bac10307-AgNPs) extracted from <i>Lactobacillus acidophilus</i> (<i>L. acidophilus</i>), which may have the potential to increase the bioactivity of bacteriocins and overcome the hurdles. It was found that extracted and purified Bac10307 had a broad range of stability for both temperature (20–100 °C) and pH (3–12). Further, based on Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS–PAGE) analysis, its molecular weight was estimated to be 4.2 kDa. The synthesized Bac10307-AgNPs showed a peak of surface plasmon resonance at 430 nm λmax. Fourier transform infrared (FTIR) confirmed the presence of biological moieties, and transmission electron microscopy (TEM) coupled with Energy dispersive X-Ray (EDX) confirmed that AgNPs were spherical and irregularly shaped, with a size range of 9–20 nm. As a result, the Bac10307-AgNPs displayed very strong antibacterial activity with MIC values as low as 8 μg/mL for <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), when compared to Bac10307 alone. In addition, Bac10307-AgNPs demonstrated promising in vitro antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC<sub>50</sub> = 116.04 μg/mL) and in vitro cytotoxicity against human liver cancer cells (HepG2) (IC<sub>50</sub> = 135.63 μg/mL), more than Bac10307 alone (IC<sub>50</sub> = 139.82 μg/mL against DPPH and 158.20 μg/mL against HepG2). Furthermore, a protein–protein molecular docking simulation study of bacteriocins with target proteins of different biological functions was also carried out in order to ascertain the interactions between bacteriocins and target proteins. |
| first_indexed | 2024-03-11T08:17:17Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T08:17:17Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-c36d7acbf278484d8631d8d7bf56982f2023-11-16T22:39:28ZengMDPI AGPharmaceutics1999-49232023-01-0115240310.3390/pharmaceutics15020403Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological ActivitiesArif Jamal Siddiqui0Mitesh Patel1Mohd Adnan2Sadaf Jahan3Juhi Saxena4Mohammed Merae Alshahrani5Abdelmushin Abdelgadir6Fevzi Bardakci7Manojkumar Sachidanandan8Riadh Badraoui9Mejdi Snoussi10Allal Ouhtit11Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, IndiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Biotechnology, University Institute of Biotechnology, Chandigarh University, Gharuan, NH-95, Ludhiana-Chandigarh State Hwy, Punjab 140413, IndiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medial Sciences, Najran University, 1988, Najran 61441, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Oral Radiology, College of Dentistry, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, QatarThe proteinaceous compounds produced by lactic acid bacteria are called bacteriocins and have a wide variety of bioactive properties. However, bacteriocin’s commercial availability is limited due to short stability periods and low yields. Therefore, the objective of this study was to synthesize bacteriocin-derived silver nanoparticles (Bac10307-AgNPs) extracted from <i>Lactobacillus acidophilus</i> (<i>L. acidophilus</i>), which may have the potential to increase the bioactivity of bacteriocins and overcome the hurdles. It was found that extracted and purified Bac10307 had a broad range of stability for both temperature (20–100 °C) and pH (3–12). Further, based on Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS–PAGE) analysis, its molecular weight was estimated to be 4.2 kDa. The synthesized Bac10307-AgNPs showed a peak of surface plasmon resonance at 430 nm λmax. Fourier transform infrared (FTIR) confirmed the presence of biological moieties, and transmission electron microscopy (TEM) coupled with Energy dispersive X-Ray (EDX) confirmed that AgNPs were spherical and irregularly shaped, with a size range of 9–20 nm. As a result, the Bac10307-AgNPs displayed very strong antibacterial activity with MIC values as low as 8 μg/mL for <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), when compared to Bac10307 alone. In addition, Bac10307-AgNPs demonstrated promising in vitro antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC<sub>50</sub> = 116.04 μg/mL) and in vitro cytotoxicity against human liver cancer cells (HepG2) (IC<sub>50</sub> = 135.63 μg/mL), more than Bac10307 alone (IC<sub>50</sub> = 139.82 μg/mL against DPPH and 158.20 μg/mL against HepG2). Furthermore, a protein–protein molecular docking simulation study of bacteriocins with target proteins of different biological functions was also carried out in order to ascertain the interactions between bacteriocins and target proteins.https://www.mdpi.com/1999-4923/15/2/403bacteria<i>L. acidophilus</i>silver nanoparticlescancerbacteriocinsTEM |
| spellingShingle | Arif Jamal Siddiqui Mitesh Patel Mohd Adnan Sadaf Jahan Juhi Saxena Mohammed Merae Alshahrani Abdelmushin Abdelgadir Fevzi Bardakci Manojkumar Sachidanandan Riadh Badraoui Mejdi Snoussi Allal Ouhtit Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities Pharmaceutics bacteria <i>L. acidophilus</i> silver nanoparticles cancer bacteriocins TEM |
| title | Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities |
| title_full | Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities |
| title_fullStr | Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities |
| title_full_unstemmed | Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities |
| title_short | Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities |
| title_sort | bacteriocin nanoconjugates bac10307 agnps biosynthesized from i lactobacillus acidophilus i derived bacteriocins exhibit enhanced and promising biological activities |
| topic | bacteria <i>L. acidophilus</i> silver nanoparticles cancer bacteriocins TEM |
| url | https://www.mdpi.com/1999-4923/15/2/403 |
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