RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis
Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that induces diarrhea and death in neonatal piglets, resulting in substantial economic losses to the global swine industry. The mechanisms of PEDV infection and the roles of host factors are still under exploration. In this st...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/1999-4915/15/10/2080 |
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| author | Yingguang Li Yuwei Bao Yan Li Xiaoxiao Duan Shaoming Dong Jiaxu Lin Xiaoyun Chang Yue Tan Hongliang Zhang Hu Shan |
| author_facet | Yingguang Li Yuwei Bao Yan Li Xiaoxiao Duan Shaoming Dong Jiaxu Lin Xiaoyun Chang Yue Tan Hongliang Zhang Hu Shan |
| author_sort | Yingguang Li |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that induces diarrhea and death in neonatal piglets, resulting in substantial economic losses to the global swine industry. The mechanisms of PEDV infection and the roles of host factors are still under exploration. In this study, we used the ferroptosis pathway downstream target activator (1S,3R)-RSL3 compound as a starting point, combined with the interactions of N-acetylcysteine and deferoxamine, to elucidate the effects of a series of compounds on PEDV proliferation. We also established glutathione peroxidase 4 (GPX4) gene overexpression to further elucidate the relationship between the ferroptosis pathway and PEDV. (1S,3R)-RSL3 inhibited PEDV replication in Vero cells, while N-acetylcysteine and deferoxamine promoted its proliferation. In addition, (1S,3R)-RSL3 mainly affected the replication stage of PEDV. Overexpression of GPX4 promoted PEDV proliferation, indicating that the ferroptosis pathway could influence PEDV replication in Vero cells. This study focused on the mechanism of (1S,3R)-RSL3 inhibition on PEDV, laying the foundation for exploring the pathogenic mechanisms of PEDV and drug development. |
| first_indexed | 2024-03-10T20:50:13Z |
| format | Article |
| id | doaj.art-c36f08a938ae4994896d4c42006e85b6 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:50:13Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c36f08a938ae4994896d4c42006e85b62023-11-19T18:27:59ZengMDPI AGViruses1999-49152023-10-011510208010.3390/v15102080RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating FerroptosisYingguang Li0Yuwei Bao1Yan Li2Xiaoxiao Duan3Shaoming Dong4Jiaxu Lin5Xiaoyun Chang6Yue Tan7Hongliang Zhang8Hu Shan9College of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaQingdao Animal Disease Prevention and Control Center, Qingdao 266100, ChinaQingdao Animal Disease Prevention and Control Center, Qingdao 266100, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao 266109, ChinaPorcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that induces diarrhea and death in neonatal piglets, resulting in substantial economic losses to the global swine industry. The mechanisms of PEDV infection and the roles of host factors are still under exploration. In this study, we used the ferroptosis pathway downstream target activator (1S,3R)-RSL3 compound as a starting point, combined with the interactions of N-acetylcysteine and deferoxamine, to elucidate the effects of a series of compounds on PEDV proliferation. We also established glutathione peroxidase 4 (GPX4) gene overexpression to further elucidate the relationship between the ferroptosis pathway and PEDV. (1S,3R)-RSL3 inhibited PEDV replication in Vero cells, while N-acetylcysteine and deferoxamine promoted its proliferation. In addition, (1S,3R)-RSL3 mainly affected the replication stage of PEDV. Overexpression of GPX4 promoted PEDV proliferation, indicating that the ferroptosis pathway could influence PEDV replication in Vero cells. This study focused on the mechanism of (1S,3R)-RSL3 inhibition on PEDV, laying the foundation for exploring the pathogenic mechanisms of PEDV and drug development.https://www.mdpi.com/1999-4915/15/10/2080(1S,3R)-RSL3GPX4porcine epidemic diarrhea virusferroptosis |
| spellingShingle | Yingguang Li Yuwei Bao Yan Li Xiaoxiao Duan Shaoming Dong Jiaxu Lin Xiaoyun Chang Yue Tan Hongliang Zhang Hu Shan RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis Viruses (1S,3R)-RSL3 GPX4 porcine epidemic diarrhea virus ferroptosis |
| title | RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis |
| title_full | RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis |
| title_fullStr | RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis |
| title_full_unstemmed | RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis |
| title_short | RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis |
| title_sort | rsl3 inhibits porcine epidemic diarrhea virus replication by activating ferroptosis |
| topic | (1S,3R)-RSL3 GPX4 porcine epidemic diarrhea virus ferroptosis |
| url | https://www.mdpi.com/1999-4915/15/10/2080 |
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