Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production
Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; <i>L. plantarum</i>, <i>L. salivarius, L. reuteri, L. brevi,</i> and <i>S. bovis</i>)...
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MDPI AG
2021-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/6/100 |
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| author | Ehsan Oskoueian Mohammad Faseleh Jahromi Saeid Jafari Majid Shakeri Hieu Huu Le Mahdi Ebrahimi |
| author_facet | Ehsan Oskoueian Mohammad Faseleh Jahromi Saeid Jafari Majid Shakeri Hieu Huu Le Mahdi Ebrahimi |
| author_sort | Ehsan Oskoueian |
| collection | DOAJ |
| description | Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; <i>L. plantarum</i>, <i>L. salivarius, L. reuteri, L. brevi,</i> and <i>S. bovis</i>) inoculation (10<sup>6</sup> cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved <i>(p</i> < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility <i>(p</i> < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for <i>L. plantarum</i> and <i>S. bovis</i>) improved silage quality, rumen fermentation parameters and microbial populations in vitro. |
| first_indexed | 2024-03-10T10:43:15Z |
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| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T10:43:15Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-c36f4ac6d06c4146883d59da413b3b1c2023-11-21T22:50:29ZengMDPI AGVeterinary Sciences2306-73812021-06-018610010.3390/vetsci8060100Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane ProductionEhsan Oskoueian0Mohammad Faseleh Jahromi1Saeid Jafari2Majid Shakeri3Hieu Huu Le4Mahdi Ebrahimi5Department of Research and Development, Arka Biotechnology Corporation, Mashhad 1696700, IranDepartment of Research and Development, Arka Biotechnology Corporation, Mashhad 1696700, IranDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Medicine, The University of Washington, Seattle, WA 98109, USAFaculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, University Putra Malaysia, Serdang 43400, Selangor, MalaysiaBacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; <i>L. plantarum</i>, <i>L. salivarius, L. reuteri, L. brevi,</i> and <i>S. bovis</i>) inoculation (10<sup>6</sup> cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved <i>(p</i> < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility <i>(p</i> < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for <i>L. plantarum</i> and <i>S. bovis</i>) improved silage quality, rumen fermentation parameters and microbial populations in vitro.https://www.mdpi.com/2306-7381/8/6/100in vitrolactic acid bacteriamethanemicrobial populationrice straw silagerumen |
| spellingShingle | Ehsan Oskoueian Mohammad Faseleh Jahromi Saeid Jafari Majid Shakeri Hieu Huu Le Mahdi Ebrahimi Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production Veterinary Sciences in vitro lactic acid bacteria methane microbial population rice straw silage rumen |
| title | Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production |
| title_full | Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production |
| title_fullStr | Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production |
| title_full_unstemmed | Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production |
| title_short | Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production |
| title_sort | manipulation of rice straw silage fermentation with different types of lactic acid bacteria inoculant affects rumen microbial fermentation characteristics and methane production |
| topic | in vitro lactic acid bacteria methane microbial population rice straw silage rumen |
| url | https://www.mdpi.com/2306-7381/8/6/100 |
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