Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
Abstract Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, alm...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53980-7 |
| _version_ | 1797274890278010880 |
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| author | Carl Dinter Andreas Gumprecht Matthias Alexander Menze Amizon Azizan Paul-Joachim Niehoff Sven Hansen Jochen Büchs |
| author_facet | Carl Dinter Andreas Gumprecht Matthias Alexander Menze Amizon Azizan Paul-Joachim Niehoff Sven Hansen Jochen Büchs |
| author_sort | Carl Dinter |
| collection | DOAJ |
| description | Abstract Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclusively concentrated on fluids at waterlike viscosity. The importance of CFD model validation cannot be overstated. While techniques to elucidate the internal flow field are necessary for model validation in STRs, the liquid distribution, caused by the orbital shaking motion of shake flasks, can be exploited for model validation. An OpenFOAM CFD model for shake flasks has been established. Calculated liquid distributions were compared to suitable, previously published experimental data. Across a broad range of shaking conditions, at waterlike and moderate viscosity (16.7 mPa∙s), the CFD model's liquid distributions align excellently with the experimental data, in terms of overall shape and position of the liquid relative to the direction of the centrifugal force. Additionally, the CFD model was used to calculate the volumetric power input, based on the energy dissipation. Depending on the shaking conditions, the computed volumetric power inputs range from 0.1 to 7 kW/m3 and differed on average by 0.01 kW/m3 from measured literature data. |
| first_indexed | 2024-03-07T15:04:38Z |
| format | Article |
| id | doaj.art-c3725940f3a94fc2952a9f8d205dfd12 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:04:38Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c3725940f3a94fc2952a9f8d205dfd122024-03-05T18:57:35ZengNature PortfolioScientific Reports2045-23222024-02-0114111710.1038/s41598-024-53980-7Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputsCarl Dinter0Andreas Gumprecht1Matthias Alexander Menze2Amizon Azizan3Paul-Joachim Niehoff4Sven Hansen5Jochen Büchs6RWTH Aachen UniversityEvonik Operations GmbHRWTH Aachen UniversitySchool of Chemical Engineering, College of Engineering, Universiti Teknologi MARARWTH Aachen UniversityEvonik Operations GmbHRWTH Aachen UniversityAbstract Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclusively concentrated on fluids at waterlike viscosity. The importance of CFD model validation cannot be overstated. While techniques to elucidate the internal flow field are necessary for model validation in STRs, the liquid distribution, caused by the orbital shaking motion of shake flasks, can be exploited for model validation. An OpenFOAM CFD model for shake flasks has been established. Calculated liquid distributions were compared to suitable, previously published experimental data. Across a broad range of shaking conditions, at waterlike and moderate viscosity (16.7 mPa∙s), the CFD model's liquid distributions align excellently with the experimental data, in terms of overall shape and position of the liquid relative to the direction of the centrifugal force. Additionally, the CFD model was used to calculate the volumetric power input, based on the energy dissipation. Depending on the shaking conditions, the computed volumetric power inputs range from 0.1 to 7 kW/m3 and differed on average by 0.01 kW/m3 from measured literature data.https://doi.org/10.1038/s41598-024-53980-7 |
| spellingShingle | Carl Dinter Andreas Gumprecht Matthias Alexander Menze Amizon Azizan Paul-Joachim Niehoff Sven Hansen Jochen Büchs Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs Scientific Reports |
| title | Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs |
| title_full | Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs |
| title_fullStr | Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs |
| title_full_unstemmed | Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs |
| title_short | Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs |
| title_sort | validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs |
| url | https://doi.org/10.1038/s41598-024-53980-7 |
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