Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice
To screen the in vitro uric acid-lowering activity of the neutral polysaccharides (NCSP) and acid polysaccharides (ACSP) isolated from the corn silk were evaluated. Furthermore, preliminary structure-activity relationships for them were investigated. The molecular weight (Mw) of NCSP and ACSP were 7...
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| Format: | Article |
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Elsevier
2021-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621003479 |
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| author | Xizhu Wang Liyan Yuan Zhijie Bao Baoshang Fu Pengfei Jiang Tiecheng Ma Songyi Lin |
| author_facet | Xizhu Wang Liyan Yuan Zhijie Bao Baoshang Fu Pengfei Jiang Tiecheng Ma Songyi Lin |
| author_sort | Xizhu Wang |
| collection | DOAJ |
| description | To screen the in vitro uric acid-lowering activity of the neutral polysaccharides (NCSP) and acid polysaccharides (ACSP) isolated from the corn silk were evaluated. Furthermore, preliminary structure-activity relationships for them were investigated. The molecular weight (Mw) of NCSP and ACSP were 74.1 and 109.4 kDa, respectively. NCSP consisted of mannose (Man), glucuronic acid (GlcA), glucose (Glc), galactose (Gal), and arabinose (Ara), in addition to these monosaccharides, the ACSP contains galactose acid (GalA). NMR spectroscopy showed that the NCSP main chain consisted of 1,4,6)-α-D-Glcp, 1,4)-α-D-Glcp, 1,3)-β-D-Galp, and 1,3,5)-β-D-Manp. NCSP exhibited higher activity in anti-hyperuricemia (HUA). The results indicated that serum uric acid (UA) and liver xanthine oxidase (XO) activities levels decreased by 45.71% and 22.4% in the NCSP groups, respectively. In addition, NCSP could significantly improve the kidney damage and promote uric acid excretion. These findings provide evidence in support of NCSP as a potential dietary supplement for improving HUA. |
| first_indexed | 2024-12-21T16:39:15Z |
| format | Article |
| id | doaj.art-c374a7833b834a99b6c6f7c463ba20eb |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T16:39:15Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-c374a7833b834a99b6c6f7c463ba20eb2022-12-21T18:57:08ZengElsevierJournal of Functional Foods1756-46462021-11-0186104698Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia miceXizhu Wang0Liyan Yuan1Zhijie Bao2Baoshang Fu3Pengfei Jiang4Tiecheng Ma5Songyi Lin6National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaCorresponding author at: No. 41 Yuqing Street, Ganjingzi District, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.; National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaTo screen the in vitro uric acid-lowering activity of the neutral polysaccharides (NCSP) and acid polysaccharides (ACSP) isolated from the corn silk were evaluated. Furthermore, preliminary structure-activity relationships for them were investigated. The molecular weight (Mw) of NCSP and ACSP were 74.1 and 109.4 kDa, respectively. NCSP consisted of mannose (Man), glucuronic acid (GlcA), glucose (Glc), galactose (Gal), and arabinose (Ara), in addition to these monosaccharides, the ACSP contains galactose acid (GalA). NMR spectroscopy showed that the NCSP main chain consisted of 1,4,6)-α-D-Glcp, 1,4)-α-D-Glcp, 1,3)-β-D-Galp, and 1,3,5)-β-D-Manp. NCSP exhibited higher activity in anti-hyperuricemia (HUA). The results indicated that serum uric acid (UA) and liver xanthine oxidase (XO) activities levels decreased by 45.71% and 22.4% in the NCSP groups, respectively. In addition, NCSP could significantly improve the kidney damage and promote uric acid excretion. These findings provide evidence in support of NCSP as a potential dietary supplement for improving HUA.http://www.sciencedirect.com/science/article/pii/S1756464621003479Neutral corn silk polysaccharidesAcid corn silk polysaccharidesStructure-activity relationshipsUric acid, hyperuricemiaXanthine oxidase |
| spellingShingle | Xizhu Wang Liyan Yuan Zhijie Bao Baoshang Fu Pengfei Jiang Tiecheng Ma Songyi Lin Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice Journal of Functional Foods Neutral corn silk polysaccharides Acid corn silk polysaccharides Structure-activity relationships Uric acid, hyperuricemia Xanthine oxidase |
| title | Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice |
| title_full | Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice |
| title_fullStr | Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice |
| title_full_unstemmed | Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice |
| title_short | Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice |
| title_sort | screening of uric acid lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice |
| topic | Neutral corn silk polysaccharides Acid corn silk polysaccharides Structure-activity relationships Uric acid, hyperuricemia Xanthine oxidase |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621003479 |
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