The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
ABSTRACT: Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal h...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123001128 |
| _version_ | 1797837190626017280 |
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| author | Shaoming Fang Jing Liu Suhong Wei Guofeng Yang Xinzhu Chen Yuxin Tong Pingting Guo |
| author_facet | Shaoming Fang Jing Liu Suhong Wei Guofeng Yang Xinzhu Chen Yuxin Tong Pingting Guo |
| author_sort | Shaoming Fang |
| collection | DOAJ |
| description | ABSTRACT: Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and production performance of goose. Hence, histomorphological assay was used for observing development status of proventriculus, jejunum, and cecum in 70-day-old Changle geese, whereas digesta from 6 alimentary canal locations (crop, proventriculus, gizzard, jejunum, cecum, and rectum) were collected for 16S rRNA gene sequencing and short chain fatty acids (SCFAs) quantitative analysis. The histomorphological observation indicated that the jejunum and cecum of Changle goose were well developed. The alpha diversity analysis revealed that, except rectum, microbiota in other noncecum sections were in high diversity as cecum. The Nonmetric MultiDimensional Scaling (NMDS) analysis showed that microbial community of proventriculus, gizzard, and jejunum formed a cluster, which distinctly discrete with the microbiota of the other gastrointestinal locations. Additionally, the proportions of Proteobacteria, Bacteroidota, and Campilobacterota at the phylum level and Lactobacillus, Streptococcus, Helicobacter, and Subdoligranulum at the genus level exhibited tremendous alternations among different gastrointestinal locations. The characteristic bacterial composition in each section was further disclosed by analyzing the core and feature Amplicon Sequence Variants (ASVs) and SCFAs pattern. Importantly, 7 body-weight-associated ASVs and 2 cecum-development-related ASVs were identified via correlation analysis. In a whole, our findings provided the first insights into the specialized digestive physiology of Changle geese and distinctive regional distribution of gastrointestinal microbiota, which laid the important foundation for improving growth performance through microbiota manipulation in geese. |
| first_indexed | 2024-04-09T15:21:23Z |
| format | Article |
| id | doaj.art-c374c707ff1240aa9f4e7752c861c895 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-09T15:21:23Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c374c707ff1240aa9f4e7752c861c8952023-04-29T14:45:36ZengElsevierPoultry Science0032-57912023-05-011025102588The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle gooseShaoming Fang0Jing Liu1Suhong Wei2Guofeng Yang3Xinzhu Chen4Yuxin Tong5Pingting Guo6Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCollege of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCollege of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding author:ABSTRACT: Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and production performance of goose. Hence, histomorphological assay was used for observing development status of proventriculus, jejunum, and cecum in 70-day-old Changle geese, whereas digesta from 6 alimentary canal locations (crop, proventriculus, gizzard, jejunum, cecum, and rectum) were collected for 16S rRNA gene sequencing and short chain fatty acids (SCFAs) quantitative analysis. The histomorphological observation indicated that the jejunum and cecum of Changle goose were well developed. The alpha diversity analysis revealed that, except rectum, microbiota in other noncecum sections were in high diversity as cecum. The Nonmetric MultiDimensional Scaling (NMDS) analysis showed that microbial community of proventriculus, gizzard, and jejunum formed a cluster, which distinctly discrete with the microbiota of the other gastrointestinal locations. Additionally, the proportions of Proteobacteria, Bacteroidota, and Campilobacterota at the phylum level and Lactobacillus, Streptococcus, Helicobacter, and Subdoligranulum at the genus level exhibited tremendous alternations among different gastrointestinal locations. The characteristic bacterial composition in each section was further disclosed by analyzing the core and feature Amplicon Sequence Variants (ASVs) and SCFAs pattern. Importantly, 7 body-weight-associated ASVs and 2 cecum-development-related ASVs were identified via correlation analysis. In a whole, our findings provided the first insights into the specialized digestive physiology of Changle geese and distinctive regional distribution of gastrointestinal microbiota, which laid the important foundation for improving growth performance through microbiota manipulation in geese.http://www.sciencedirect.com/science/article/pii/S0032579123001128Changle goosegastrointestinal microbiotaregional distributiondigestive physiology |
| spellingShingle | Shaoming Fang Jing Liu Suhong Wei Guofeng Yang Xinzhu Chen Yuxin Tong Pingting Guo The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose Poultry Science Changle goose gastrointestinal microbiota regional distribution digestive physiology |
| title | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
| title_full | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
| title_fullStr | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
| title_full_unstemmed | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
| title_short | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
| title_sort | integrated analysis of digestive physiology and gastrointestinal microbiota structure in changle goose |
| topic | Changle goose gastrointestinal microbiota regional distribution digestive physiology |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123001128 |
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