The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats
The cafeteria (CAF) diet, reflective of predominant Western dietary behaviors, is implicated in hastening weight gain, subsequently resulting in health complications such as obesity, diabetes, and cancer. To this end, it is vital to notice the deleterious consequences of the CAF regimen prior to the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/21/4557 |
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| author | Nurdan Aba Enver Fehim Koçpınar Taha Ceylani |
| author_facet | Nurdan Aba Enver Fehim Koçpınar Taha Ceylani |
| author_sort | Nurdan Aba |
| collection | DOAJ |
| description | The cafeteria (CAF) diet, reflective of predominant Western dietary behaviors, is implicated in hastening weight gain, subsequently resulting in health complications such as obesity, diabetes, and cancer. To this end, it is vital to notice the deleterious consequences of the CAF regimen prior to the onset of complications, which is fundamental for early intervention in the context of numerous diseases. Probiotic-derived postbiotic metabolites have gained attention for their antioxidative properties, offering a potential countermeasure against oxidative stress. This research sought to discern the protective efficacy of SCD Probiotics against liver glutathione system damage arising from the CAF diet during developmental phases. Male Wistar rats, from weaning on day 21 to day 56, were categorized into four groups: a control on a conventional diet; a group on a standard diet enriched with SCD Probiotics; a mixed-diet group comprising both CAF and standard feed; and a combination diet group supplemented with SCD Probiotics. Through the application of real-time PCR, enzyme activity assessments, and quantitative metabolite analyses, our findings highlight the CAF diet’s adverse influence on the liver’s antioxidant defenses via shifts in gene expression. Yet, the inclusion of SCD Probiotics mostly ameliorated these harmful effects. Remarkably, the positive regulatory influence of SCD Probiotics on the liver’s antioxidant system was consistently observed, independent of the CAF diet’s presence. |
| first_indexed | 2024-03-11T11:24:42Z |
| format | Article |
| id | doaj.art-c3759e9b802f4dbbbb7e0084b7176efd |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T11:24:42Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c3759e9b802f4dbbbb7e0084b7176efd2023-11-10T15:09:36ZengMDPI AGNutrients2072-66432023-10-011521455710.3390/nu15214557The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar RatsNurdan Aba0Enver Fehim Koçpınar1Taha Ceylani2Department of Biology, Science Faculty, Muş Alparslan University, 49250 Mus, TurkeyDepartment of Medical Laboratory Techniques, Vocational School of Health Services, Muş Alparslan University, 49250 Mus, TurkeyDepartment of Food Quality Control and Analysis, Muş Alparslan University, 49250 Mus, TurkeyThe cafeteria (CAF) diet, reflective of predominant Western dietary behaviors, is implicated in hastening weight gain, subsequently resulting in health complications such as obesity, diabetes, and cancer. To this end, it is vital to notice the deleterious consequences of the CAF regimen prior to the onset of complications, which is fundamental for early intervention in the context of numerous diseases. Probiotic-derived postbiotic metabolites have gained attention for their antioxidative properties, offering a potential countermeasure against oxidative stress. This research sought to discern the protective efficacy of SCD Probiotics against liver glutathione system damage arising from the CAF diet during developmental phases. Male Wistar rats, from weaning on day 21 to day 56, were categorized into four groups: a control on a conventional diet; a group on a standard diet enriched with SCD Probiotics; a mixed-diet group comprising both CAF and standard feed; and a combination diet group supplemented with SCD Probiotics. Through the application of real-time PCR, enzyme activity assessments, and quantitative metabolite analyses, our findings highlight the CAF diet’s adverse influence on the liver’s antioxidant defenses via shifts in gene expression. Yet, the inclusion of SCD Probiotics mostly ameliorated these harmful effects. Remarkably, the positive regulatory influence of SCD Probiotics on the liver’s antioxidant system was consistently observed, independent of the CAF diet’s presence.https://www.mdpi.com/2072-6643/15/21/4557antioxidant systemcafeteria dietSCD probioticsgene expressionenzyme activity |
| spellingShingle | Nurdan Aba Enver Fehim Koçpınar Taha Ceylani The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats Nutrients antioxidant system cafeteria diet SCD probiotics gene expression enzyme activity |
| title | The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats |
| title_full | The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats |
| title_fullStr | The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats |
| title_full_unstemmed | The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats |
| title_short | The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats |
| title_sort | hepatic antioxidant system damage induced with the cafeteria caf diet is largely counteracted using scd probiotics during development of male wistar rats |
| topic | antioxidant system cafeteria diet SCD probiotics gene expression enzyme activity |
| url | https://www.mdpi.com/2072-6643/15/21/4557 |
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