Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis
Atopic dermatitis is an inflammatory skin disease characterized by significant permeability barrier damage. Regulation and maintenance of permeability and antimicrobial skin barriers are strongly connected. There is a lack of comprehensive studies of the expression of all 5 major antimicrobial pepti...
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| Format: | Article |
| Language: | English |
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Medical Journals Sweden
2023-06-01
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| Series: | Acta Dermato-Venereologica |
| Subjects: | |
| Online Access: | https://medicaljournalssweden.se/actadv/article/view/9413 |
| _version_ | 1797791335120371712 |
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| author | Lilla Szabó Anikó Kapitány Orsolya Somogyi Iman Alhafez Krisztián Gáspár Réka Palatka Lilla Soltész Dániel Törőcsik Zoltán Hendrik Zsolt Dajnoki Andrea Szegedi |
| author_facet | Lilla Szabó Anikó Kapitány Orsolya Somogyi Iman Alhafez Krisztián Gáspár Réka Palatka Lilla Soltész Dániel Törőcsik Zoltán Hendrik Zsolt Dajnoki Andrea Szegedi |
| author_sort | Lilla Szabó |
| collection | DOAJ |
| description | Atopic dermatitis is an inflammatory skin disease characterized by significant permeability barrier damage. Regulation and maintenance of permeability and antimicrobial skin barriers are strongly connected. There is a lack of comprehensive studies of the expression of all 5 major antimicrobial peptide functional groups in atopic dermatitis. The aim of this study was to investigate the major antimicrobial peptide functional groups in lesional atopic dermatitis, non-lesional atopic dermatitis, and healthy control samples, using real-time quantitative PCR and immunohistochemistry. Lesional psoriatic skin was also examined as a diseased control. No differences in mRNA levels were detected between non-lesional atopic dermatitis and healthy control skin, and, at the protein level, the only change was the significantly decreased LL-37 in non-lesional atopic dermatitis. In lesional atopic dermatitis, several antimicrobial peptides were significantly altered at the mRNA level, while, at the protein level, all antimicrobial peptides were significantly upregulated or unchanged, except for LL-37, which decreased, compared with healthy controls. Antimicrobial peptides were similarly elevated in lesional atopic dermatitis and lesional psoriatic skin, with somewhat higher expression in lesional psoriatic skin, except for LL-37. In conclusion, LL-37 was the only antimicrobial peptide that was impaired in both non-lesional and lesional atopic dermatitis, highlighting its potential pathogenetic or exacerbating role in the initial stages of the disease.
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| first_indexed | 2024-03-13T02:18:19Z |
| format | Article |
| id | doaj.art-c375c83ebcc54ca79f065ad0ef42e037 |
| institution | Directory Open Access Journal |
| issn | 0001-5555 1651-2057 |
| language | English |
| last_indexed | 2024-03-13T02:18:19Z |
| publishDate | 2023-06-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Acta Dermato-Venereologica |
| spelling | doaj.art-c375c83ebcc54ca79f065ad0ef42e0372023-06-30T11:29:07ZengMedical Journals SwedenActa Dermato-Venereologica0001-55551651-20572023-06-0110310.2340/actadv.v103.9413Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic DermatitisLilla Szabó0Anikó Kapitány1Orsolya Somogyi2Iman Alhafez3Krisztián Gáspár4Réka Palatka5Lilla Soltész6Dániel Törőcsik7Zoltán Hendrik8Zsolt Dajnoki9Andrea Szegedi10Department of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, University of Debrecen, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; ELKH-DE Allergology Research Group, Debrecen, Hungary Department of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, University of Debrecen, Debrecen, Hungary; ELKH-DE Allergology Research Group, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, University of Debrecen, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, University of Debrecen, Debrecen, HungaryDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; ELKH-DE Allergology Research Group, Debrecen, Hungary Department of Forensic Medicine, Faculty of Medicine, University of Debrecen, Debrecen, HungaryDepertment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, DebrecenDepartment of Dermatology, Centre of Excellence, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; ELKH-DE Allergology Research Group, Debrecen, Hungary Atopic dermatitis is an inflammatory skin disease characterized by significant permeability barrier damage. Regulation and maintenance of permeability and antimicrobial skin barriers are strongly connected. There is a lack of comprehensive studies of the expression of all 5 major antimicrobial peptide functional groups in atopic dermatitis. The aim of this study was to investigate the major antimicrobial peptide functional groups in lesional atopic dermatitis, non-lesional atopic dermatitis, and healthy control samples, using real-time quantitative PCR and immunohistochemistry. Lesional psoriatic skin was also examined as a diseased control. No differences in mRNA levels were detected between non-lesional atopic dermatitis and healthy control skin, and, at the protein level, the only change was the significantly decreased LL-37 in non-lesional atopic dermatitis. In lesional atopic dermatitis, several antimicrobial peptides were significantly altered at the mRNA level, while, at the protein level, all antimicrobial peptides were significantly upregulated or unchanged, except for LL-37, which decreased, compared with healthy controls. Antimicrobial peptides were similarly elevated in lesional atopic dermatitis and lesional psoriatic skin, with somewhat higher expression in lesional psoriatic skin, except for LL-37. In conclusion, LL-37 was the only antimicrobial peptide that was impaired in both non-lesional and lesional atopic dermatitis, highlighting its potential pathogenetic or exacerbating role in the initial stages of the disease. https://medicaljournalssweden.se/actadv/article/view/9413Antimicrobial peptideAtopic dermatitisSkin barrierPsoriasis |
| spellingShingle | Lilla Szabó Anikó Kapitány Orsolya Somogyi Iman Alhafez Krisztián Gáspár Réka Palatka Lilla Soltész Dániel Törőcsik Zoltán Hendrik Zsolt Dajnoki Andrea Szegedi Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis Acta Dermato-Venereologica Antimicrobial peptide Atopic dermatitis Skin barrier Psoriasis |
| title | Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis |
| title_full | Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis |
| title_fullStr | Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis |
| title_full_unstemmed | Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis |
| title_short | Antimicrobial Peptide Loss, Except for LL-37, is not Characteristic of Atopic Dermatitis |
| title_sort | antimicrobial peptide loss except for ll 37 is not characteristic of atopic dermatitis |
| topic | Antimicrobial peptide Atopic dermatitis Skin barrier Psoriasis |
| url | https://medicaljournalssweden.se/actadv/article/view/9413 |
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