Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers
The genetic diversity and population structure studies on 32 wild Malus species were conducted using the nine inter simple sequence repeat (ISSR) markers. The average value of diversity indices, viz. resolving power (Rp), polymorphic information content (PIC), effective multiplex ratio (EMR) and mar...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-07-01
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| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/91650 |
| _version_ | 1797896975876620288 |
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| author | CHAVLESH KUMAR S K SINGH RAKESH SINGH M K VERMA K K PRAMANICK SRIVASTAV SRIVASTAV RAMESH KUMAR J K VERMA NARENDER NEGI |
| author_facet | CHAVLESH KUMAR S K SINGH RAKESH SINGH M K VERMA K K PRAMANICK SRIVASTAV SRIVASTAV RAMESH KUMAR J K VERMA NARENDER NEGI |
| author_sort | CHAVLESH KUMAR |
| collection | DOAJ |
| description | The genetic diversity and population structure studies on 32 wild Malus species were conducted using the nine inter simple sequence repeat (ISSR) markers. The average value of diversity indices, viz. resolving power (Rp), polymorphic information content (PIC), effective multiplex ratio (EMR) and marker index (MI) of ISSR markers were 2.389, 0.388, 16.429 and 6.228, respectively, while the Jaccard’s similarity coefficient ranged from 0.46 to 0.97. The cluster analysis divided the selected Malus species into two major clusters and principal coordinate analysis (PCoA) further reconfirmed the result of the cluster analysis. The first three axes of PCoA explained 43.49% variation and analysis of molecular variance (AMOVA) explained 16% variation between the indigenous and exotic Malus populations. The studied Malus species were genetically differentiated into four distinct populations which were revealed through the model based population structure analysis. Thus, the present investigation revealed substantial genetic diversity among the studied wild Malus species and existing genetic diversity could be valuable genetic resources for future apple improvement programme. |
| first_indexed | 2024-04-10T07:51:22Z |
| format | Article |
| id | doaj.art-c3764708609d42fabe5993e9bc311ff9 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T07:51:22Z |
| publishDate | 2019-07-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-c3764708609d42fabe5993e9bc311ff92023-02-23T10:14:14ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192019-07-0189710.56093/ijas.v89i7.91650Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markersCHAVLESH KUMAR0S K SINGH1RAKESH SINGH2M K VERMA3K K PRAMANICK4SRIVASTAV SRIVASTAV5RAMESH KUMAR6J K VERMA7NARENDER NEGI8Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaDivision of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaDivision of Genomic Resources, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaDivision of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaICAR-IARI Regional Station, Amartara Cottage, ShimlaICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaDivision of Genomic Resources, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaICAR-IARI Regional Station, Amartara Cottage, ShimlaICAR-NBPGR Regional Station, Phagali, ShimlaThe genetic diversity and population structure studies on 32 wild Malus species were conducted using the nine inter simple sequence repeat (ISSR) markers. The average value of diversity indices, viz. resolving power (Rp), polymorphic information content (PIC), effective multiplex ratio (EMR) and marker index (MI) of ISSR markers were 2.389, 0.388, 16.429 and 6.228, respectively, while the Jaccard’s similarity coefficient ranged from 0.46 to 0.97. The cluster analysis divided the selected Malus species into two major clusters and principal coordinate analysis (PCoA) further reconfirmed the result of the cluster analysis. The first three axes of PCoA explained 43.49% variation and analysis of molecular variance (AMOVA) explained 16% variation between the indigenous and exotic Malus populations. The studied Malus species were genetically differentiated into four distinct populations which were revealed through the model based population structure analysis. Thus, the present investigation revealed substantial genetic diversity among the studied wild Malus species and existing genetic diversity could be valuable genetic resources for future apple improvement programme.https://epubs.icar.org.in/index.php/IJAgS/article/view/91650CrabapplesGenetic diversityIndigenousISSR markersWild Malus species |
| spellingShingle | CHAVLESH KUMAR S K SINGH RAKESH SINGH M K VERMA K K PRAMANICK SRIVASTAV SRIVASTAV RAMESH KUMAR J K VERMA NARENDER NEGI Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers The Indian Journal of Agricultural Sciences Crabapples Genetic diversity Indigenous ISSR markers Wild Malus species |
| title | Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers |
| title_full | Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers |
| title_fullStr | Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers |
| title_full_unstemmed | Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers |
| title_short | Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers |
| title_sort | analysis of genetic diversity and population structure of the indigenous and exotic wild malus species using issr markers |
| topic | Crabapples Genetic diversity Indigenous ISSR markers Wild Malus species |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/91650 |
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