Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality
The contribution of the apple microbiome to the production chain of apple was so far largely unknown. Here, we describe the apple fruit microbiome and influences on its composition by parameters such as storage season, storage duration, storage technology, apple variety, and plant protection schemes...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/2/272 |
| _version_ | 1797406260023263232 |
|---|---|
| author | Yvonne Bösch Elisabeth Britt Sarah Perren Andreas Naef Jürg E. Frey Andreas Bühlmann |
| author_facet | Yvonne Bösch Elisabeth Britt Sarah Perren Andreas Naef Jürg E. Frey Andreas Bühlmann |
| author_sort | Yvonne Bösch |
| collection | DOAJ |
| description | The contribution of the apple microbiome to the production chain of apple was so far largely unknown. Here, we describe the apple fruit microbiome and influences on its composition by parameters such as storage season, storage duration, storage technology, apple variety, and plant protection schemes. A combined culturing and metabarcoding approach revealed significant differences in the abundance, composition, and diversity of the apple fruit microbiome. We showed that relatively few genera contribute a large portion of the microbiome on fruit and that the fruit microbiome changes during the storage season depending on the storage conditions. In addition, we show that the plant protection regime has an influence on the diversity of the fruit microbiome and on the dynamics of pathogenic fungal genera during the storage season. For the genus <i>Neofabraea,</i> the quantitative results from the metabarcoding approach were validated with real-time PCR. In conclusion, we identified key parameters determining the composition and temporal changes of the apple fruit microbiome, and the main abiotic driving factors of microbiome diversity on apple fruit were characterized. |
| first_indexed | 2024-03-09T03:23:51Z |
| format | Article |
| id | doaj.art-c377cd110e4347fc9b5d48e0e6436a1b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:23:51Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c377cd110e4347fc9b5d48e0e6436a1b2023-12-03T15:05:39ZengMDPI AGMicroorganisms2076-26072021-01-019227210.3390/microorganisms9020272Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit QualityYvonne Bösch0Elisabeth Britt1Sarah Perren2Andreas Naef3Jürg E. Frey4Andreas Bühlmann5Competence Division Plants and Plant Products, Agroscope, Müller-Thurgaustr 29, 8820 Wädenswil, SwitzerlandCompetence Division Plants and Plant Products, Agroscope, Müller-Thurgaustr 29, 8820 Wädenswil, SwitzerlandCompetence Division Plants and Plant Products, Agroscope, Müller-Thurgaustr 29, 8820 Wädenswil, SwitzerlandCompetence Division Plants and Plant Products, Agroscope, Müller-Thurgaustr 29, 8820 Wädenswil, SwitzerlandCompetence Division Method Development and Analytics, Agroscope, Müller-Thurgaustr 29, 8820 Wädenswil, SwitzerlandCompetence Division Plants and Plant Products, Agroscope, Müller-Thurgaustr 29, 8820 Wädenswil, SwitzerlandThe contribution of the apple microbiome to the production chain of apple was so far largely unknown. Here, we describe the apple fruit microbiome and influences on its composition by parameters such as storage season, storage duration, storage technology, apple variety, and plant protection schemes. A combined culturing and metabarcoding approach revealed significant differences in the abundance, composition, and diversity of the apple fruit microbiome. We showed that relatively few genera contribute a large portion of the microbiome on fruit and that the fruit microbiome changes during the storage season depending on the storage conditions. In addition, we show that the plant protection regime has an influence on the diversity of the fruit microbiome and on the dynamics of pathogenic fungal genera during the storage season. For the genus <i>Neofabraea,</i> the quantitative results from the metabarcoding approach were validated with real-time PCR. In conclusion, we identified key parameters determining the composition and temporal changes of the apple fruit microbiome, and the main abiotic driving factors of microbiome diversity on apple fruit were characterized.https://www.mdpi.com/2076-2607/9/2/272applefruit microbiomefruit quality<i>Neofabraea</i> spp. |
| spellingShingle | Yvonne Bösch Elisabeth Britt Sarah Perren Andreas Naef Jürg E. Frey Andreas Bühlmann Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality Microorganisms apple fruit microbiome fruit quality <i>Neofabraea</i> spp. |
| title | Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality |
| title_full | Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality |
| title_fullStr | Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality |
| title_full_unstemmed | Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality |
| title_short | Dynamics of the Apple Fruit Microbiome after Harvest and Implications for Fruit Quality |
| title_sort | dynamics of the apple fruit microbiome after harvest and implications for fruit quality |
| topic | apple fruit microbiome fruit quality <i>Neofabraea</i> spp. |
| url | https://www.mdpi.com/2076-2607/9/2/272 |
| work_keys_str_mv | AT yvonnebosch dynamicsoftheapplefruitmicrobiomeafterharvestandimplicationsforfruitquality AT elisabethbritt dynamicsoftheapplefruitmicrobiomeafterharvestandimplicationsforfruitquality AT sarahperren dynamicsoftheapplefruitmicrobiomeafterharvestandimplicationsforfruitquality AT andreasnaef dynamicsoftheapplefruitmicrobiomeafterharvestandimplicationsforfruitquality AT jurgefrey dynamicsoftheapplefruitmicrobiomeafterharvestandimplicationsforfruitquality AT andreasbuhlmann dynamicsoftheapplefruitmicrobiomeafterharvestandimplicationsforfruitquality |