Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency
Background: Derivation of induced pluripotent stem cells (iPSCs) from various adult somatic cells through over-expression of pluripotent genes could allow for the unlimited autologous supply in regenerative medicine. On the other hand the generation of various progenitors from bone-marrow mesenchyma...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2016-04-01
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| Series: | International Journal of Organ Transplantation Medicine |
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| Online Access: | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/296 |
| _version_ | 1818235556047355904 |
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| author | T Foroutan |
| author_facet | T Foroutan |
| author_sort | T Foroutan |
| collection | DOAJ |
| description | Background: Derivation of induced pluripotent stem cells (iPSCs) from various adult somatic cells through over-expression of pluripotent genes could allow for the unlimited autologous supply in regenerative medicine. On the other hand the generation of various progenitors from bone-marrow mesenchymal stem cells (MSCs) is justly well established.
Objective: In this study we compared the expression level of pluripotent genes oct4, c-myc, sox-2, nanog, klf4 and lin28 in iPSCs and MSCs derived from bone marrow. Also the potential of osteogenesis of iPSCs and bone-marrow MSCs were compared.
Methods: We analyzed the expression level of oct4, sox-2, c-myc, klf4, nanog and lin28 genes in human MSCs derived from iPSCs and MSCs by cell culture and real-time PCR. Also the expression level of osteocalcin and osteopontin in both groups were evaluated.
Results: We found that the expression of osteogenic markers in differentiated iPSCs to osteoblast were higher than bone-marrow MSCs. While the levels of pluripotency genes oct4, c-myc and klf4 in iPSCs were significantly (p<0.05) higher than bone-marrow MSCs, MSCs showed higher expression of sox-2, nanog and lin28 compared with iPSCs (p=NS).
Conclusion: It seems that the higher expression of osteopontin and osteocalcin in MSCs compared to iPSCs may be due to other factors (besides pluripotency) required for differentiation of stem cells to osteoblast. |
| first_indexed | 2024-12-12T11:55:50Z |
| format | Article |
| id | doaj.art-c377dc01eb624f65bf3bd99edeebb5df |
| institution | Directory Open Access Journal |
| issn | 2008-6482 2008-6490 |
| language | English |
| last_indexed | 2024-12-12T11:55:50Z |
| publishDate | 2016-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | International Journal of Organ Transplantation Medicine |
| spelling | doaj.art-c377dc01eb624f65bf3bd99edeebb5df2022-12-22T00:25:12ZengShiraz University of Medical SciencesInternational Journal of Organ Transplantation Medicine2008-64822008-64902016-04-0172210Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> PluripotencyT Foroutan0Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranBackground: Derivation of induced pluripotent stem cells (iPSCs) from various adult somatic cells through over-expression of pluripotent genes could allow for the unlimited autologous supply in regenerative medicine. On the other hand the generation of various progenitors from bone-marrow mesenchymal stem cells (MSCs) is justly well established. Objective: In this study we compared the expression level of pluripotent genes oct4, c-myc, sox-2, nanog, klf4 and lin28 in iPSCs and MSCs derived from bone marrow. Also the potential of osteogenesis of iPSCs and bone-marrow MSCs were compared. Methods: We analyzed the expression level of oct4, sox-2, c-myc, klf4, nanog and lin28 genes in human MSCs derived from iPSCs and MSCs by cell culture and real-time PCR. Also the expression level of osteocalcin and osteopontin in both groups were evaluated. Results: We found that the expression of osteogenic markers in differentiated iPSCs to osteoblast were higher than bone-marrow MSCs. While the levels of pluripotency genes oct4, c-myc and klf4 in iPSCs were significantly (p<0.05) higher than bone-marrow MSCs, MSCs showed higher expression of sox-2, nanog and lin28 compared with iPSCs (p=NS). Conclusion: It seems that the higher expression of osteopontin and osteocalcin in MSCs compared to iPSCs may be due to other factors (besides pluripotency) required for differentiation of stem cells to osteoblast.http://www.ijotm.com/ojs/index.php/IJOTM/article/view/296Induced pluripotent stem cellsMesenchymal stromal cellsGene expression profilingOsteogenesisXite transcript, mouse [Supplementary Concept]Genes, mycsox2 protein, xenopus [Supplementary Concept] |
| spellingShingle | T Foroutan Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency International Journal of Organ Transplantation Medicine Induced pluripotent stem cells Mesenchymal stromal cells Gene expression profiling Osteogenesis Xite transcript, mouse [Supplementary Concept] Genes, myc sox2 protein, xenopus [Supplementary Concept] |
| title | Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency |
| title_full | Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency |
| title_fullStr | Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency |
| title_full_unstemmed | Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency |
| title_short | Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis <em>versus</em> Pluripotency |
| title_sort | comparison of differentiation of induced pluripotent stem cells and bone marrow mesenchymal stem cells to osteoblast osteogenesis em versus em pluripotency |
| topic | Induced pluripotent stem cells Mesenchymal stromal cells Gene expression profiling Osteogenesis Xite transcript, mouse [Supplementary Concept] Genes, myc sox2 protein, xenopus [Supplementary Concept] |
| url | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/296 |
| work_keys_str_mv | AT tforoutan comparisonofdifferentiationofinducedpluripotentstemcellsandbonemarrowmesenchymalstemcellstoosteoblastosteogenesisemversusempluripotency |