Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro
Plant regeneration via somatic embryogenesis or organogenesis is a key for manipulation of somatic cells in vitro. Organogenesis from leaf inoculum, petiole and stem segments of in vitro black potato (Solenostemon rotundifolius (Poir) JK Morton) is influenced by the balance of the concentration of...
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| Format: | Article |
| Language: | English |
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Indonesian Biological Society
2012-10-01
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| Series: | Journal of Biological Researches |
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| Online Access: | http://berkalahayati.org/journal/cc138f8d |
| _version_ | 1818513574834733056 |
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| author | Tatin Suherlina Aryani Leksonowati Witjaksono Witjaksono |
| author_facet | Tatin Suherlina Aryani Leksonowati Witjaksono Witjaksono |
| author_sort | Tatin Suherlina |
| collection | DOAJ |
| description | Plant regeneration via somatic embryogenesis or organogenesis is a key for manipulation of somatic cells in vitro. Organogenesis from leaf inoculum, petiole and stem segments of in vitro black potato (Solenostemon rotundifolius (Poir) JK Morton) is influenced by the balance of the concentration of BA and NAA in the culture medium. However, organogenesis could also be affected by factors inherent to the inoculums. This paper reports the influence of leaf position on the in vitro shoots and culture age on the formation and production of shoot adventives from leaf inoculum. Leaves excised from shoots of 3-8 week-old culture and leaf position 1-5 from apical to basal was used as inocula. The highest percentage (83.3%) of adventitious bud develops at cultures age of 5 weeks with the average number of shoots 6.1. The highest percentage of bud development occurs at leaf position number 2-4, with the percentage of buds of 83.3, 76.2 and 76.2, respectively with the average number of shoots of 3.19, 2.10 dan 2.38 respectively. Organogenesis that produces the highest shoot production of 18.6 per Petri dish was obtained from leaves derived from the 2ndleaf from apical of 5 week-old culture inoculated on MS medium enriched with 5 mg/l BA and 0.1 mg/l NAA. |
| first_indexed | 2024-12-11T00:03:19Z |
| format | Article |
| id | doaj.art-c3782f89b0954d84a1e658f029559415 |
| institution | Directory Open Access Journal |
| issn | 0852-6834 2337-389X |
| language | English |
| last_indexed | 2024-12-11T00:03:19Z |
| publishDate | 2012-10-01 |
| publisher | Indonesian Biological Society |
| record_format | Article |
| series | Journal of Biological Researches |
| spelling | doaj.art-c3782f89b0954d84a1e658f0295594152022-12-22T01:28:24ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2012-10-011719710110.23869/bphjbr.17.1.201119Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In VitroTatin Suherlina0Aryani Leksonowati1Witjaksono Witjaksono2Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: (021) 8765066/(021) 8765067Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: (021) 8765066/(021) 8765067Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: (021) 8765066/(021) 8765067Plant regeneration via somatic embryogenesis or organogenesis is a key for manipulation of somatic cells in vitro. Organogenesis from leaf inoculum, petiole and stem segments of in vitro black potato (Solenostemon rotundifolius (Poir) JK Morton) is influenced by the balance of the concentration of BA and NAA in the culture medium. However, organogenesis could also be affected by factors inherent to the inoculums. This paper reports the influence of leaf position on the in vitro shoots and culture age on the formation and production of shoot adventives from leaf inoculum. Leaves excised from shoots of 3-8 week-old culture and leaf position 1-5 from apical to basal was used as inocula. The highest percentage (83.3%) of adventitious bud develops at cultures age of 5 weeks with the average number of shoots 6.1. The highest percentage of bud development occurs at leaf position number 2-4, with the percentage of buds of 83.3, 76.2 and 76.2, respectively with the average number of shoots of 3.19, 2.10 dan 2.38 respectively. Organogenesis that produces the highest shoot production of 18.6 per Petri dish was obtained from leaves derived from the 2ndleaf from apical of 5 week-old culture inoculated on MS medium enriched with 5 mg/l BA and 0.1 mg/l NAA.http://berkalahayati.org/journal/cc138f8din vitro cultureleaf positionorganogenesisSolenostemon rotundifolius (Poir) JK MortonCulture ageIn vitro cultureLeaf positionOrganogenesis |
| spellingShingle | Tatin Suherlina Aryani Leksonowati Witjaksono Witjaksono Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro Journal of Biological Researches in vitro culture leaf position organogenesis Solenostemon rotundifolius (Poir) JK Morton Culture age In vitro culture Leaf position Organogenesis |
| title | Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro |
| title_full | Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro |
| title_fullStr | Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro |
| title_full_unstemmed | Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro |
| title_short | Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro |
| title_sort | pengaruh umur biak dan posisi daun terhadap morfogenesis dari daun kentang hitam solenostemon rotundifolius poir jk morton in vitro |
| topic | in vitro culture leaf position organogenesis Solenostemon rotundifolius (Poir) JK Morton Culture age In vitro culture Leaf position Organogenesis |
| url | http://berkalahayati.org/journal/cc138f8d |
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